Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis

Hwang et al. demonstrate that a high axial resolution can be achieved even with low numerical aperture (NA) objectives. They show the nano-profile of a basal cell membrane using metal-induced energy transfer and biexponential fluorescence lifetime analysis. The low-NA objective provides a larger fie...

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Auteurs principaux:	Wonsang Hwang, Jinwon Seo, DongEun Kim, Chang Jun Lee, In-Hong Choi, Kyung-Hwa Yoo, Dug Young Kim
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/f6a9c48e3b55426f81cce5742a76bd43
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spelling	oai:doaj.org-article:f6a9c48e3b55426f81cce5742a76bd432021-12-02T13:51:05ZLarge field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis10.1038/s42003-020-01628-32399-3642https://doaj.org/article/f6a9c48e3b55426f81cce5742a76bd432021-01-01T00:00:00Zhttps://doi.org/10.1038/s42003-020-01628-3https://doaj.org/toc/2399-3642Hwang et al. demonstrate that a high axial resolution can be achieved even with low numerical aperture (NA) objectives. They show the nano-profile of a basal cell membrane using metal-induced energy transfer and biexponential fluorescence lifetime analysis. The low-NA objective provides a larger field-of-view (FOV), thereby overcoming the limitations of a small FOV of the usually used high-NA objectives.Wonsang HwangJinwon SeoDongEun KimChang Jun LeeIn-Hong ChoiKyung-Hwa YooDug Young KimNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Wonsang Hwang Jinwon Seo DongEun Kim Chang Jun Lee In-Hong Choi Kyung-Hwa Yoo Dug Young Kim Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis
description	Hwang et al. demonstrate that a high axial resolution can be achieved even with low numerical aperture (NA) objectives. They show the nano-profile of a basal cell membrane using metal-induced energy transfer and biexponential fluorescence lifetime analysis. The low-NA objective provides a larger field-of-view (FOV), thereby overcoming the limitations of a small FOV of the usually used high-NA objectives.
format	article
author	Wonsang Hwang Jinwon Seo DongEun Kim Chang Jun Lee In-Hong Choi Kyung-Hwa Yoo Dug Young Kim
author_facet	Wonsang Hwang Jinwon Seo DongEun Kim Chang Jun Lee In-Hong Choi Kyung-Hwa Yoo Dug Young Kim
author_sort	Wonsang Hwang
title	Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis
title_short	Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis
title_full	Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis
title_fullStr	Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis
title_full_unstemmed	Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis
title_sort	large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f6a9c48e3b55426f81cce5742a76bd43
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Large field-of-view nanometer-sectioning microscopy by using metal-induced energy transfer and biexponential lifetime analysis

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