Capturing and concentrating adenovirus using magnetic anionic nanobeads

Akikazu Sakudo,1 Koichi Baba,2 Kazuyoshi Ikuta1,31Department of Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka, Japan; 2Baba Pediatric Clinic, Kadoma, Osaka, Japan; 3Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sakudo A, Baba K, Ikuta K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	anionic polymer virus concentration adenovirus capture magnetic beads poly(methyl vinyl ether-maleic anhydrate) Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f6aa26d142444053a15d77a16ffef40a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f6aa26d142444053a15d77a16ffef40a
record_format	dspace
spelling	oai:doaj.org-article:f6aa26d142444053a15d77a16ffef40a2021-12-02T05:14:28ZCapturing and concentrating adenovirus using magnetic anionic nanobeads1178-2013https://doaj.org/article/f6aa26d142444053a15d77a16ffef40a2016-05-01T00:00:00Zhttps://www.dovepress.com/capturing-and-concentrating-adenovirus-using-magnetic-anionic-nanobead-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Akikazu Sakudo,1 Koichi Baba,2 Kazuyoshi Ikuta1,31Department of Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka, Japan; 2Baba Pediatric Clinic, Kadoma, Osaka, Japan; 3Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa, JapanAbstract: We recently demonstrated how various enveloped viruses can be efficiently concentrated using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). However, the exact mechanism of interaction between the virus particles and anionic beads remains unclear. To further investigate whether these magnetic anionic beads specifically bind to the viral envelope, we examined their potential interaction with a nonenveloped virus (adenovirus). The beads were incubated with either adenovirus-infected cell culture medium or nasal aspirates from adenovirus-infected individuals and then separated from the supernatant by applying a magnetic field. After thoroughly washing the beads, adsorption of adenovirus was confirmed by a variety of techniques, including immunochromatography, polymerase chain reaction, Western blotting, and cell culture infection assays. These detection methods positively identified the hexon and penton capsid proteins of adenovirus along with the viral genome on the magnetic beads. Furthermore, various types of adenovirus including Types 5, 6, 11, 19, and 41 were captured using the magnetic bead procedure. Our bead capture method was also found to increase the sensitivity of viral detection. Adenovirus below the detectable limit for immunochromatography was efficiently concentrated using the magnetic bead procedure, allowing the virus to be successfully detected using this methodology. Moreover, these findings clearly demonstrate that a viral envelope is not required for binding to the anionic magnetic beads. Taken together, our results show that this capture procedure increases the sensitivity of detection of adenovirus and would, therefore, be a valuable tool for analyzing both clinical and experimental samples.Keywords: anionic polymer, virus concentration, adenovirus, capture, magnetic beads, poly(methyl vinyl ether-maleic anhydrate)Sakudo ABaba KIkuta KDove Medical Pressarticleanionic polymervirus concentrationadenoviruscapturemagnetic beadspoly(methyl vinyl ether-maleic anhydrate)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1847-1857 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	anionic polymer virus concentration adenovirus capture magnetic beads poly(methyl vinyl ether-maleic anhydrate) Medicine (General) R5-920
spellingShingle	anionic polymer virus concentration adenovirus capture magnetic beads poly(methyl vinyl ether-maleic anhydrate) Medicine (General) R5-920 Sakudo A Baba K Ikuta K Capturing and concentrating adenovirus using magnetic anionic nanobeads
description	Akikazu Sakudo,1 Koichi Baba,2 Kazuyoshi Ikuta1,31Department of Virology, Research Institute for Microbial Diseases, Osaka University, Yamadaoka, Suita, Osaka, Japan; 2Baba Pediatric Clinic, Kadoma, Osaka, Japan; 3Kanonji Institute, The Research Foundation for Microbial Diseases of Osaka University, Kanonji, Kagawa, JapanAbstract: We recently demonstrated how various enveloped viruses can be efficiently concentrated using magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic anhydrate). However, the exact mechanism of interaction between the virus particles and anionic beads remains unclear. To further investigate whether these magnetic anionic beads specifically bind to the viral envelope, we examined their potential interaction with a nonenveloped virus (adenovirus). The beads were incubated with either adenovirus-infected cell culture medium or nasal aspirates from adenovirus-infected individuals and then separated from the supernatant by applying a magnetic field. After thoroughly washing the beads, adsorption of adenovirus was confirmed by a variety of techniques, including immunochromatography, polymerase chain reaction, Western blotting, and cell culture infection assays. These detection methods positively identified the hexon and penton capsid proteins of adenovirus along with the viral genome on the magnetic beads. Furthermore, various types of adenovirus including Types 5, 6, 11, 19, and 41 were captured using the magnetic bead procedure. Our bead capture method was also found to increase the sensitivity of viral detection. Adenovirus below the detectable limit for immunochromatography was efficiently concentrated using the magnetic bead procedure, allowing the virus to be successfully detected using this methodology. Moreover, these findings clearly demonstrate that a viral envelope is not required for binding to the anionic magnetic beads. Taken together, our results show that this capture procedure increases the sensitivity of detection of adenovirus and would, therefore, be a valuable tool for analyzing both clinical and experimental samples.Keywords: anionic polymer, virus concentration, adenovirus, capture, magnetic beads, poly(methyl vinyl ether-maleic anhydrate)
format	article
author	Sakudo A Baba K Ikuta K
author_facet	Sakudo A Baba K Ikuta K
author_sort	Sakudo A
title	Capturing and concentrating adenovirus using magnetic anionic nanobeads
title_short	Capturing and concentrating adenovirus using magnetic anionic nanobeads
title_full	Capturing and concentrating adenovirus using magnetic anionic nanobeads
title_fullStr	Capturing and concentrating adenovirus using magnetic anionic nanobeads
title_full_unstemmed	Capturing and concentrating adenovirus using magnetic anionic nanobeads
title_sort	capturing and concentrating adenovirus using magnetic anionic nanobeads
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/f6aa26d142444053a15d77a16ffef40a
work_keys_str_mv	AT sakudoa capturingandconcentratingadenovirususingmagneticanionicnanobeads AT babak capturingandconcentratingadenovirususingmagneticanionicnanobeads AT ikutak capturingandconcentratingadenovirususingmagneticanionicnanobeads
_version_	1718400508801056768

Capturing and concentrating adenovirus using magnetic anionic nanobeads

Ejemplares similares