The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells

Abstract Glioblastoma multiforme (GBM) is an extremely aggressive brain tumor, characterized by its high genetic heterogeneity. In search of novel putative therapeutic RNA targets we investigated the role of the oncogenic long noncoding RNA LINC00152 (CYTOR, and STAiR18) in A172 glioblastoma cells....

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Autores principales:	Stefanie Binder, Ivonne Zipfel, Claudia Müller, Karolin Wiedemann, Carolin Schimmelpfennig, Gabriele Pfeifer, Kristin Reiche, Sunna Hauschildt, Jörg Lehmann, Ulrike Köhl, Friedemann Horn, Maik Friedrich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6aa5d7c6fd84bb49a2909a2f8daa5fa
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spelling	oai:doaj.org-article:f6aa5d7c6fd84bb49a2909a2f8daa5fa2021-12-02T18:02:15ZThe noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells10.1038/s41598-021-97533-82045-2322https://doaj.org/article/f6aa5d7c6fd84bb49a2909a2f8daa5fa2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97533-8https://doaj.org/toc/2045-2322Abstract Glioblastoma multiforme (GBM) is an extremely aggressive brain tumor, characterized by its high genetic heterogeneity. In search of novel putative therapeutic RNA targets we investigated the role of the oncogenic long noncoding RNA LINC00152 (CYTOR, and STAiR18) in A172 glioblastoma cells. Here, we are the first to describe, that LINC00152 unexpectedly acts in a tumor suppressive manner in this cell line. SiRNA-based knockdown of LINC00152 enhanced malignant tumor behaviors including proliferation, cell cycle entry, migration, and invasion, contradicting previous studies using U87-MG and LN229 glioblastoma cells. Furthermore, LINC00152 knockdown had no influence on survival of A172 glioblastoma cells. In a genome wide transcription analysis of A172 and U87-MG glioblastoma cells, we identified 70 LINC00152 target genes involved in locomotion, cell migration, and motility in A172 cells, whereas in U87-MG cells only 40 target genes were detected. The LINC00152-regulated genes found in A172 differed from those identified in U87-MG glioblastoma cells, none of them being regulated in both cell lines. These findings underline the strong genetic heterogeneity of glioblastoma and point to a potential, yet unknown risk addressing LINC00152 lncRNA as a prospective therapeutic target in GBM.Stefanie BinderIvonne ZipfelClaudia MüllerKarolin WiedemannCarolin SchimmelpfennigGabriele PfeiferKristin ReicheSunna HauschildtJörg LehmannUlrike KöhlFriedemann HornMaik FriedrichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefanie Binder Ivonne Zipfel Claudia Müller Karolin Wiedemann Carolin Schimmelpfennig Gabriele Pfeifer Kristin Reiche Sunna Hauschildt Jörg Lehmann Ulrike Köhl Friedemann Horn Maik Friedrich The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells
description	Abstract Glioblastoma multiforme (GBM) is an extremely aggressive brain tumor, characterized by its high genetic heterogeneity. In search of novel putative therapeutic RNA targets we investigated the role of the oncogenic long noncoding RNA LINC00152 (CYTOR, and STAiR18) in A172 glioblastoma cells. Here, we are the first to describe, that LINC00152 unexpectedly acts in a tumor suppressive manner in this cell line. SiRNA-based knockdown of LINC00152 enhanced malignant tumor behaviors including proliferation, cell cycle entry, migration, and invasion, contradicting previous studies using U87-MG and LN229 glioblastoma cells. Furthermore, LINC00152 knockdown had no influence on survival of A172 glioblastoma cells. In a genome wide transcription analysis of A172 and U87-MG glioblastoma cells, we identified 70 LINC00152 target genes involved in locomotion, cell migration, and motility in A172 cells, whereas in U87-MG cells only 40 target genes were detected. The LINC00152-regulated genes found in A172 differed from those identified in U87-MG glioblastoma cells, none of them being regulated in both cell lines. These findings underline the strong genetic heterogeneity of glioblastoma and point to a potential, yet unknown risk addressing LINC00152 lncRNA as a prospective therapeutic target in GBM.
format	article
author	Stefanie Binder Ivonne Zipfel Claudia Müller Karolin Wiedemann Carolin Schimmelpfennig Gabriele Pfeifer Kristin Reiche Sunna Hauschildt Jörg Lehmann Ulrike Köhl Friedemann Horn Maik Friedrich
author_facet	Stefanie Binder Ivonne Zipfel Claudia Müller Karolin Wiedemann Carolin Schimmelpfennig Gabriele Pfeifer Kristin Reiche Sunna Hauschildt Jörg Lehmann Ulrike Köhl Friedemann Horn Maik Friedrich
author_sort	Stefanie Binder
title	The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells
title_short	The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells
title_full	The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells
title_fullStr	The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells
title_full_unstemmed	The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells
title_sort	noncoding rna linc00152 conveys contradicting effects in different glioblastoma cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f6aa5d7c6fd84bb49a2909a2f8daa5fa
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The noncoding RNA LINC00152 conveys contradicting effects in different glioblastoma cells

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