Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta

Abstract The properties of incident light play a crucial role in the mating process of diatoms, a group of ecologically important microalgae. While species-specific requirements for light intensity and photoperiod have been observed in several diatom species, little is known about the light spectrum...

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Autores principales:	Gust Bilcke, Lore Van Craenenbroeck, Alexandre Castagna, Cristina Maria Osuna-Cruz, Klaas Vandepoele, Koen Sabbe, Lieven De Veylder, Wim Vyverman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6b2190e925c4079a89044326bf06094
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spelling	oai:doaj.org-article:f6b2190e925c4079a89044326bf060942021-12-02T17:51:21ZLight intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta10.1038/s41598-021-92838-02045-2322https://doaj.org/article/f6b2190e925c4079a89044326bf060942021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92838-0https://doaj.org/toc/2045-2322Abstract The properties of incident light play a crucial role in the mating process of diatoms, a group of ecologically important microalgae. While species-specific requirements for light intensity and photoperiod have been observed in several diatom species, little is known about the light spectrum that allows sexual reproduction. Here, we study the effects of spectral properties and light intensity on the initiation and progression of sexual reproduction in the model benthic diatom Seminavis robusta. We found that distinct stages of the mating process have different requirements for light. Vigorous mating pair formation occurred under a broad range of light intensities, ranging from 10 to 81 µE m−2 s−1, while gametogenesis and subsequent stages were strongly affected by moderate light intensities of 27 µE m−2 s−1 and up. In addition, light of blue or blue–green wavelengths was required for the formation of mating pairs. Combining flow cytometric analysis with expression profiling of the diatom-specific cyclin dsCyc2 suggests that progression through a blue light-dependent checkpoint in the G1 cell cycle phase is essential for induction of sexual reproduction. Taken together, we expand the current model of mating in benthic pennate diatoms, which relies on the interplay between light, cell cycle and sex pheromone signaling.Gust BilckeLore Van CraenenbroeckAlexandre CastagnaCristina Maria Osuna-CruzKlaas VandepoeleKoen SabbeLieven De VeylderWim VyvermanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gust Bilcke Lore Van Craenenbroeck Alexandre Castagna Cristina Maria Osuna-Cruz Klaas Vandepoele Koen Sabbe Lieven De Veylder Wim Vyverman Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta
description	Abstract The properties of incident light play a crucial role in the mating process of diatoms, a group of ecologically important microalgae. While species-specific requirements for light intensity and photoperiod have been observed in several diatom species, little is known about the light spectrum that allows sexual reproduction. Here, we study the effects of spectral properties and light intensity on the initiation and progression of sexual reproduction in the model benthic diatom Seminavis robusta. We found that distinct stages of the mating process have different requirements for light. Vigorous mating pair formation occurred under a broad range of light intensities, ranging from 10 to 81 µE m−2 s−1, while gametogenesis and subsequent stages were strongly affected by moderate light intensities of 27 µE m−2 s−1 and up. In addition, light of blue or blue–green wavelengths was required for the formation of mating pairs. Combining flow cytometric analysis with expression profiling of the diatom-specific cyclin dsCyc2 suggests that progression through a blue light-dependent checkpoint in the G1 cell cycle phase is essential for induction of sexual reproduction. Taken together, we expand the current model of mating in benthic pennate diatoms, which relies on the interplay between light, cell cycle and sex pheromone signaling.
format	article
author	Gust Bilcke Lore Van Craenenbroeck Alexandre Castagna Cristina Maria Osuna-Cruz Klaas Vandepoele Koen Sabbe Lieven De Veylder Wim Vyverman
author_facet	Gust Bilcke Lore Van Craenenbroeck Alexandre Castagna Cristina Maria Osuna-Cruz Klaas Vandepoele Koen Sabbe Lieven De Veylder Wim Vyverman
author_sort	Gust Bilcke
title	Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta
title_short	Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta
title_full	Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta
title_fullStr	Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta
title_full_unstemmed	Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta
title_sort	light intensity and spectral composition drive reproductive success in the marine benthic diatom seminavis robusta
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f6b2190e925c4079a89044326bf06094
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Light intensity and spectral composition drive reproductive success in the marine benthic diatom Seminavis robusta

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