Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model

Epithelial–mesenchymal transition (EMT) occurs in the early stages of embryonic development and plays a significant role in the migration and the differentiation of cells into various types of tissues of an organism. However, tumor cells, with altered form and function, use the EMT process to migrat...

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Autores principales:	Ellen Emi Kato, Sandra Coccuzzo Sampaio
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	crotoxin epithelial–mesenchymal transition spheroid model tumor stroma Medicine R
Acceso en línea:	https://doaj.org/article/f6b26b6954c3409d81dd60f24a5c69bd
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spelling	oai:doaj.org-article:f6b26b6954c3409d81dd60f24a5c69bd2021-11-25T19:09:12ZCrotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model10.3390/toxins131108302072-6651https://doaj.org/article/f6b26b6954c3409d81dd60f24a5c69bd2021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/830https://doaj.org/toc/2072-6651Epithelial–mesenchymal transition (EMT) occurs in the early stages of embryonic development and plays a significant role in the migration and the differentiation of cells into various types of tissues of an organism. However, tumor cells, with altered form and function, use the EMT process to migrate and invade other tissues in the body. Several experimental (in vivo and in vitro) and clinical trial studies have shown the antitumor activity of crotoxin (CTX), a heterodimeric phospholipase A2 present in the <i>Crotalus durissus terrificus</i> venom. In this study, we show that CTX modulates the microenvironment of tumor cells. We have also evaluated the effect of CTX on the EMT process in the spheroid model. The invasion of type I collagen gels by heterospheroids (mix of MRC-5 and A549 cells constitutively prepared with 12.5 nM CTX), expression of EMT markers, and secretion of MMPs were analyzed. Western blotting analysis shows that CTX inhibits the expression of the mesenchymal markers, N-cadherin, α-SMA, and αv. This study provides evidence of CTX as a key modulator of the EMT process, and its antitumor action can be explored further for novel drug designing against metastatic cancer.Ellen Emi KatoSandra Coccuzzo SampaioMDPI AGarticlecrotoxinepithelial–mesenchymal transitionspheroid modeltumor stromaMedicineRENToxins, Vol 13, Iss 830, p 830 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	crotoxin epithelial–mesenchymal transition spheroid model tumor stroma Medicine R
spellingShingle	crotoxin epithelial–mesenchymal transition spheroid model tumor stroma Medicine R Ellen Emi Kato Sandra Coccuzzo Sampaio Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model
description	Epithelial–mesenchymal transition (EMT) occurs in the early stages of embryonic development and plays a significant role in the migration and the differentiation of cells into various types of tissues of an organism. However, tumor cells, with altered form and function, use the EMT process to migrate and invade other tissues in the body. Several experimental (in vivo and in vitro) and clinical trial studies have shown the antitumor activity of crotoxin (CTX), a heterodimeric phospholipase A2 present in the <i>Crotalus durissus terrificus</i> venom. In this study, we show that CTX modulates the microenvironment of tumor cells. We have also evaluated the effect of CTX on the EMT process in the spheroid model. The invasion of type I collagen gels by heterospheroids (mix of MRC-5 and A549 cells constitutively prepared with 12.5 nM CTX), expression of EMT markers, and secretion of MMPs were analyzed. Western blotting analysis shows that CTX inhibits the expression of the mesenchymal markers, N-cadherin, α-SMA, and αv. This study provides evidence of CTX as a key modulator of the EMT process, and its antitumor action can be explored further for novel drug designing against metastatic cancer.
format	article
author	Ellen Emi Kato Sandra Coccuzzo Sampaio
author_facet	Ellen Emi Kato Sandra Coccuzzo Sampaio
author_sort	Ellen Emi Kato
title	Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model
title_short	Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model
title_full	Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model
title_fullStr	Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model
title_full_unstemmed	Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model
title_sort	crotoxin modulates events involved in epithelial–mesenchymal transition in 3d spheroid model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f6b26b6954c3409d81dd60f24a5c69bd
work_keys_str_mv	AT ellenemikato crotoxinmodulateseventsinvolvedinepithelialmesenchymaltransitionin3dspheroidmodel AT sandracoccuzzosampaio crotoxinmodulateseventsinvolvedinepithelialmesenchymaltransitionin3dspheroidmodel
_version_	1718410254839971840

Crotoxin Modulates Events Involved in Epithelial–Mesenchymal Transition in 3D Spheroid Model

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