Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159

ABSTRACT Transposon mutagenesis coupled with next-generation DNA sequencing (Tn-seq) is a powerful tool for discovering regions of the genome that are required for the survival of bacteria in different environments. We adapted this technique to the dental caries pathogen Streptococcus mutans UA159 a...

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Autores principales:	Robert C. Shields, Lin Zeng, David J. Culp, Robert A. Burne
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Streptococcus antimicrobial targets dental caries essential genome transposon sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f6bb3f099fde41e29ed284e210c02913
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spelling	oai:doaj.org-article:f6bb3f099fde41e29ed284e210c029132021-11-15T15:22:02ZGenomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA15910.1128/mSphere.00031-182379-5042https://doaj.org/article/f6bb3f099fde41e29ed284e210c029132018-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00031-18https://doaj.org/toc/2379-5042ABSTRACT Transposon mutagenesis coupled with next-generation DNA sequencing (Tn-seq) is a powerful tool for discovering regions of the genome that are required for the survival of bacteria in different environments. We adapted this technique to the dental caries pathogen Streptococcus mutans UA159 and identified 11% of the genome as essential, with many genes encoding products required for replication, translation, lipid metabolism, and cell wall biogenesis. Comparison of the essential genome of S. mutans UA159 with those of selected other streptococci for which such information is available revealed several metabolic pathways and genes that are required in S. mutans, but not in some Streptococcus spp. We further identified genes that are essential for sustained growth in rich or defined medium, as well as for persistence in vivo in a rodent model of oral infection. Collectively, our results provide a novel and comprehensive view of the genes required for essential processes of S. mutans, many of which could represent potential targets for therapeutics. IMPORTANCE Tooth decay (dental caries) is a common cause of pain, impaired quality of life, and tooth loss in children and adults. It begins because of a compositional change in the microorganisms that colonize the tooth surface driven by repeated and sustained carbohydrate intake. Although several bacterial species are associated with tooth decay, Streptococcus mutans is the most common cause. Therefore, it is important to identify biological processes that contribute to the survival of S. mutans in the human mouth, with the aim of disrupting the processes with antimicrobial agents. We successfully applied Tn-seq to S. mutans, discovering genes that are required for survival, growth, and persistence, both in laboratory environments and in a mouse model of tooth decay. This work highlights new avenues for the control of an important human pathogen.Robert C. ShieldsLin ZengDavid J. CulpRobert A. BurneAmerican Society for MicrobiologyarticleStreptococcusantimicrobial targetsdental cariesessential genometransposon sequencingMicrobiologyQR1-502ENmSphere, Vol 3, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Streptococcus antimicrobial targets dental caries essential genome transposon sequencing Microbiology QR1-502
spellingShingle	Streptococcus antimicrobial targets dental caries essential genome transposon sequencing Microbiology QR1-502 Robert C. Shields Lin Zeng David J. Culp Robert A. Burne Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159
description	ABSTRACT Transposon mutagenesis coupled with next-generation DNA sequencing (Tn-seq) is a powerful tool for discovering regions of the genome that are required for the survival of bacteria in different environments. We adapted this technique to the dental caries pathogen Streptococcus mutans UA159 and identified 11% of the genome as essential, with many genes encoding products required for replication, translation, lipid metabolism, and cell wall biogenesis. Comparison of the essential genome of S. mutans UA159 with those of selected other streptococci for which such information is available revealed several metabolic pathways and genes that are required in S. mutans, but not in some Streptococcus spp. We further identified genes that are essential for sustained growth in rich or defined medium, as well as for persistence in vivo in a rodent model of oral infection. Collectively, our results provide a novel and comprehensive view of the genes required for essential processes of S. mutans, many of which could represent potential targets for therapeutics. IMPORTANCE Tooth decay (dental caries) is a common cause of pain, impaired quality of life, and tooth loss in children and adults. It begins because of a compositional change in the microorganisms that colonize the tooth surface driven by repeated and sustained carbohydrate intake. Although several bacterial species are associated with tooth decay, Streptococcus mutans is the most common cause. Therefore, it is important to identify biological processes that contribute to the survival of S. mutans in the human mouth, with the aim of disrupting the processes with antimicrobial agents. We successfully applied Tn-seq to S. mutans, discovering genes that are required for survival, growth, and persistence, both in laboratory environments and in a mouse model of tooth decay. This work highlights new avenues for the control of an important human pathogen.
format	article
author	Robert C. Shields Lin Zeng David J. Culp Robert A. Burne
author_facet	Robert C. Shields Lin Zeng David J. Culp Robert A. Burne
author_sort	Robert C. Shields
title	Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159
title_short	Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159
title_full	Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159
title_fullStr	Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159
title_full_unstemmed	Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159
title_sort	genomewide identification of essential genes and fitness determinants of <named-content content-type="genus-species">streptococcus mutans</named-content> ua159
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/f6bb3f099fde41e29ed284e210c02913
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Genomewide Identification of Essential Genes and Fitness Determinants of <named-content content-type="genus-species">Streptococcus mutans</named-content> UA159

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