The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro

Abstract Nerves are subjected to tensile forces in various paradigms such as injury and regeneration, joint movement, and rehabilitation treatments, as in the case of neurodynamic treatment (NDT). The NDT induces selective uniaxial repeated tension on the nerve and was described to be an effective t...

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Autores principales:	Giacomo Carta, Giovanna Gambarotta, Benedetta Elena Fornasari, Luisa Muratori, Marwa El Soury, Stefano Geuna, Stefania Raimondo, Federica Fregnan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6bb5a801724429b913eded8190a42f8
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spelling	oai:doaj.org-article:f6bb5a801724429b913eded8190a42f82021-12-02T17:45:18ZThe neurodynamic treatment induces biological changes in sensory and motor neurons in vitro10.1038/s41598-021-92682-22045-2322https://doaj.org/article/f6bb5a801724429b913eded8190a42f82021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92682-2https://doaj.org/toc/2045-2322Abstract Nerves are subjected to tensile forces in various paradigms such as injury and regeneration, joint movement, and rehabilitation treatments, as in the case of neurodynamic treatment (NDT). The NDT induces selective uniaxial repeated tension on the nerve and was described to be an effective treatment to reduce pain in patients. Nevertheless, the biological mechanisms activated by the NDT promoting the healing processes of the nerve are yet still unknown. Moreover, a dose–response analysis to define a standard protocol of treatment is unavailable. In this study, we aimed to define in vitro whether NDT protocols could induce selective biological effects on sensory and motor neurons, also investigating the possible involved molecular mechanisms taking a role behind this change. The obtained results demonstrate that NDT induced significant dose-dependent changes promoting cell differentiation, neurite outgrowth, and neuron survival, especially in nociceptive neurons. Notably, NDT significantly upregulated PIEZO1 gene expression. A gene that is coding for an ion channel that is expressed both in murine and human sensory neurons and is related to mechanical stimuli transduction and pain suppression. Other genes involved in mechanical allodynia related to neuroinflammation were not modified by NDT. The results of the present study contribute to increase the knowledge behind the biological mechanisms activated in response to NDT and to understand its efficacy in improving nerve regenerational physiological processes and pain reduction.Giacomo CartaGiovanna GambarottaBenedetta Elena FornasariLuisa MuratoriMarwa El SouryStefano GeunaStefania RaimondoFederica FregnanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giacomo Carta Giovanna Gambarotta Benedetta Elena Fornasari Luisa Muratori Marwa El Soury Stefano Geuna Stefania Raimondo Federica Fregnan The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro
description	Abstract Nerves are subjected to tensile forces in various paradigms such as injury and regeneration, joint movement, and rehabilitation treatments, as in the case of neurodynamic treatment (NDT). The NDT induces selective uniaxial repeated tension on the nerve and was described to be an effective treatment to reduce pain in patients. Nevertheless, the biological mechanisms activated by the NDT promoting the healing processes of the nerve are yet still unknown. Moreover, a dose–response analysis to define a standard protocol of treatment is unavailable. In this study, we aimed to define in vitro whether NDT protocols could induce selective biological effects on sensory and motor neurons, also investigating the possible involved molecular mechanisms taking a role behind this change. The obtained results demonstrate that NDT induced significant dose-dependent changes promoting cell differentiation, neurite outgrowth, and neuron survival, especially in nociceptive neurons. Notably, NDT significantly upregulated PIEZO1 gene expression. A gene that is coding for an ion channel that is expressed both in murine and human sensory neurons and is related to mechanical stimuli transduction and pain suppression. Other genes involved in mechanical allodynia related to neuroinflammation were not modified by NDT. The results of the present study contribute to increase the knowledge behind the biological mechanisms activated in response to NDT and to understand its efficacy in improving nerve regenerational physiological processes and pain reduction.
format	article
author	Giacomo Carta Giovanna Gambarotta Benedetta Elena Fornasari Luisa Muratori Marwa El Soury Stefano Geuna Stefania Raimondo Federica Fregnan
author_facet	Giacomo Carta Giovanna Gambarotta Benedetta Elena Fornasari Luisa Muratori Marwa El Soury Stefano Geuna Stefania Raimondo Federica Fregnan
author_sort	Giacomo Carta
title	The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro
title_short	The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro
title_full	The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro
title_fullStr	The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro
title_full_unstemmed	The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro
title_sort	neurodynamic treatment induces biological changes in sensory and motor neurons in vitro
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f6bb5a801724429b913eded8190a42f8
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The neurodynamic treatment induces biological changes in sensory and motor neurons in vitro

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