Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro

Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporation and, consequently, for transport of molecules...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tina Batista Napotnik, Damijan Miklavčič
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	electroporation calcium uptake electric field direction nanosecond pulses Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/f6c1fe728a174d3db63218d5e21d4e33
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f6c1fe728a174d3db63218d5e21d4e33
record_format	dspace
spelling	oai:doaj.org-article:f6c1fe728a174d3db63218d5e21d4e332021-11-11T18:33:09ZPulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro10.3390/molecules262165711420-3049https://doaj.org/article/f6c1fe728a174d3db63218d5e21d4e332021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6571https://doaj.org/toc/1420-3049Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporation and, consequently, for transport of molecules through permeabilized plasma membrane. Uptake of molecules after electroporation are the greatest at poles of cells facing electrodes and is often asymmetrical. However, asymmetry reported was inconsistent and inconclusive—in different reports it was either preferentially anodal or cathodal. We investigated the asymmetry of polar uptake of calcium ions after electroporation with electric pulses of different durations, as the orientation of elongated cells affects electroporation to a different extent when using electric pulses of different durations in the range of 100 ns to 100 µs. The results show that with 1, 10, and 100 µs pulses, the uptake of calcium ions is greater at the pole closer to the cathode than at the pole closer to the anode. With shorter 100 ns pulses, the asymmetry is not observed. A different extent of electroporation at different parts of elongated cells, such as muscle or cardiac cells, may have an impact on electroporation-based treatments such as drug delivery, pulse-field ablation, and gene electrotransfection.Tina Batista NapotnikDamijan MiklavčičMDPI AGarticleelectroporationcalcium uptakeelectric field directionnanosecond pulsesOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6571, p 6571 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	electroporation calcium uptake electric field direction nanosecond pulses Organic chemistry QD241-441
spellingShingle	electroporation calcium uptake electric field direction nanosecond pulses Organic chemistry QD241-441 Tina Batista Napotnik Damijan Miklavčič Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
description	Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporation and, consequently, for transport of molecules through permeabilized plasma membrane. Uptake of molecules after electroporation are the greatest at poles of cells facing electrodes and is often asymmetrical. However, asymmetry reported was inconsistent and inconclusive—in different reports it was either preferentially anodal or cathodal. We investigated the asymmetry of polar uptake of calcium ions after electroporation with electric pulses of different durations, as the orientation of elongated cells affects electroporation to a different extent when using electric pulses of different durations in the range of 100 ns to 100 µs. The results show that with 1, 10, and 100 µs pulses, the uptake of calcium ions is greater at the pole closer to the cathode than at the pole closer to the anode. With shorter 100 ns pulses, the asymmetry is not observed. A different extent of electroporation at different parts of elongated cells, such as muscle or cardiac cells, may have an impact on electroporation-based treatments such as drug delivery, pulse-field ablation, and gene electrotransfection.
format	article
author	Tina Batista Napotnik Damijan Miklavčič
author_facet	Tina Batista Napotnik Damijan Miklavčič
author_sort	Tina Batista Napotnik
title	Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
title_short	Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
title_full	Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
title_fullStr	Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
title_full_unstemmed	Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro
title_sort	pulse duration dependent asymmetry in molecular transmembrane transport due to electroporation in h9c2 rat cardiac myoblast cells in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f6c1fe728a174d3db63218d5e21d4e33
work_keys_str_mv	AT tinabatistanapotnik pulsedurationdependentasymmetryinmoleculartransmembranetransportduetoelectroporationinh9c2ratcardiacmyoblastcellsinvitro AT damijanmiklavcic pulsedurationdependentasymmetryinmoleculartransmembranetransportduetoelectroporationinh9c2ratcardiacmyoblastcellsinvitro
_version_	1718431798904487936

Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro

Ejemplares similares