Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study

LF Amorosa,1 CH Lee,2 AB Aydemir,1 S Nizami,1 A Hsu,1 NR Patel,1 TR Gardner,1 A Navalgund,3 D-G Kim,3 SH Park,4 JJ Mao,2 FY Lee11Center for Orthopaedic Research, Columbia University Medical Center, New York, NY, 2College of Dental Medicine, Columbia University Medical Center, New York, NY, 3Orthodon...

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Autores principales:	Amorosa LF, Lee CH, Aydemir AB, Nizami S, Hsu A, Patel NR, Gardner TR, Navalgund A, Kim D-G, Park SH, Mao JJ, Lee FY
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f6c46e023e9c4aa78227925019ac8039
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spelling	oai:doaj.org-article:f6c46e023e9c4aa78227925019ac80392021-12-02T08:45:15ZPhysiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study1176-91141178-2013https://doaj.org/article/f6c46e023e9c4aa78227925019ac80392013-04-01T00:00:00Zhttp://www.dovepress.com/physiologic-load-bearing-characteristics-of-autografts-allografts-and--a12866https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013LF Amorosa,1 CH Lee,2 AB Aydemir,1 S Nizami,1 A Hsu,1 NR Patel,1 TR Gardner,1 A Navalgund,3 D-G Kim,3 SH Park,4 JJ Mao,2 FY Lee11Center for Orthopaedic Research, Columbia University Medical Center, New York, NY, 2College of Dental Medicine, Columbia University Medical Center, New York, NY, 3Orthodontics, College of Dentistry, Ohio State University, Columbus, OH, 4Department of Orthopaedic Surgery, University of California at Los Angeles Medical Center, Los Angeles, CA, USABackground: To address the challenge of treating critical sized intercalary defects, we hypothesized that under physiologic cyclic loading, autografts, allografts, and scaffolds loaded with and without human mesenchymal stem cells (hMSCs) would have different biomechanical characteristics.Methods: Using a rat femoral defect model, 46 rats were assigned to four groups, ie, autograft (n = 12), allograft (n = 10), scaffold (n = 13), and scaffold with hMSCs (n = 11). The scaffold groups used a 5 mm segment of scaffold composed of 80% poly-ε-caprolactone and 20% hydroxyapatite. Rats were sacrificed 4 months postoperatively, and the repairs were assessed radiographically and biomechanically.Results: Autograft and allograft groups exhibited the most bridging callus, while the scaffold/hMSCs group had more callus than the scaffold repairs. Although signs of radiographic healing did not accurately reflect restoration of mechanical properties, addition of hMSCs on the scaffold enhanced bone formation. The scaffold alone group had significantly lower elastic and viscous stiffness and higher phase angles than other repairs and the contralateral controls. Addition of hMSCs increased the elastic and viscous stiffness of the repair, while decreasing the phase angle.Conclusion: Further comparative analysis is needed to optimize clinical use of scaffolds and hMSCs for critical sized defect repairs. However, our results suggest that addition of hMSCs to scaffolds enhances mechanical simulation of native host bone.Keywords: fracture healing, scaffolds, human mesenchymal stem cells, tissue engineeringAmorosa LFLee CHAydemir ABNizami SHsu APatel NRGardner TRNavalgund AKim D-GPark SHMao JJLee FYDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 1637-1643 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Amorosa LF Lee CH Aydemir AB Nizami S Hsu A Patel NR Gardner TR Navalgund A Kim D-G Park SH Mao JJ Lee FY Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
description	LF Amorosa,1 CH Lee,2 AB Aydemir,1 S Nizami,1 A Hsu,1 NR Patel,1 TR Gardner,1 A Navalgund,3 D-G Kim,3 SH Park,4 JJ Mao,2 FY Lee11Center for Orthopaedic Research, Columbia University Medical Center, New York, NY, 2College of Dental Medicine, Columbia University Medical Center, New York, NY, 3Orthodontics, College of Dentistry, Ohio State University, Columbus, OH, 4Department of Orthopaedic Surgery, University of California at Los Angeles Medical Center, Los Angeles, CA, USABackground: To address the challenge of treating critical sized intercalary defects, we hypothesized that under physiologic cyclic loading, autografts, allografts, and scaffolds loaded with and without human mesenchymal stem cells (hMSCs) would have different biomechanical characteristics.Methods: Using a rat femoral defect model, 46 rats were assigned to four groups, ie, autograft (n = 12), allograft (n = 10), scaffold (n = 13), and scaffold with hMSCs (n = 11). The scaffold groups used a 5 mm segment of scaffold composed of 80% poly-ε-caprolactone and 20% hydroxyapatite. Rats were sacrificed 4 months postoperatively, and the repairs were assessed radiographically and biomechanically.Results: Autograft and allograft groups exhibited the most bridging callus, while the scaffold/hMSCs group had more callus than the scaffold repairs. Although signs of radiographic healing did not accurately reflect restoration of mechanical properties, addition of hMSCs on the scaffold enhanced bone formation. The scaffold alone group had significantly lower elastic and viscous stiffness and higher phase angles than other repairs and the contralateral controls. Addition of hMSCs increased the elastic and viscous stiffness of the repair, while decreasing the phase angle.Conclusion: Further comparative analysis is needed to optimize clinical use of scaffolds and hMSCs for critical sized defect repairs. However, our results suggest that addition of hMSCs to scaffolds enhances mechanical simulation of native host bone.Keywords: fracture healing, scaffolds, human mesenchymal stem cells, tissue engineering
format	article
author	Amorosa LF Lee CH Aydemir AB Nizami S Hsu A Patel NR Gardner TR Navalgund A Kim D-G Park SH Mao JJ Lee FY
author_facet	Amorosa LF Lee CH Aydemir AB Nizami S Hsu A Patel NR Gardner TR Navalgund A Kim D-G Park SH Mao JJ Lee FY
author_sort	Amorosa LF
title	Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
title_short	Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
title_full	Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
title_fullStr	Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
title_full_unstemmed	Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
title_sort	physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/f6c46e023e9c4aa78227925019ac8039
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Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: an experimental study

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