Optimization of genome engineering approaches with the CRISPR/Cas9 system.
Designer nucleases such as TALENS and Cas9 have opened new opportunities to scarlessly edit the mammalian genome. Here we explored several parameters that influence Cas9-mediated scarless genome editing efficiency in murine embryonic stem cells. Optimization of transfection conditions and enriching...
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| Main Authors: | , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Public Library of Science (PLoS)
2014
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| Subjects: | |
| Online Access: | https://doaj.org/article/f6caf0c6e1134b9bb7c852fa3574dd9b |
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