Optimization of genome engineering approaches with the CRISPR/Cas9 system.
Designer nucleases such as TALENS and Cas9 have opened new opportunities to scarlessly edit the mammalian genome. Here we explored several parameters that influence Cas9-mediated scarless genome editing efficiency in murine embryonic stem cells. Optimization of transfection conditions and enriching...
Enregistré dans:
| Auteurs principaux: | , , , , |
|---|---|
| Format: | article |
| Langue: | EN |
| Publié: |
Public Library of Science (PLoS)
2014
|
| Sujets: | |
| Accès en ligne: | https://doaj.org/article/f6caf0c6e1134b9bb7c852fa3574dd9b |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|