Distinct signatures of calcium activity in brain mural cells

Pericytes have been implicated in various neuropathologies, yet little is known about their function and signaling pathways in health. Here, we characterized calcium dynamics of cortical mural cells in anesthetized or awake Pdgfrb-CreERT2;Rosa26< LSL-GCaMP6s > mice and in acute brain s...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chaim Glück, Kim David Ferrari, Noemi Binini, Annika Keller, Aiman S Saab, Jillian L Stobart, Bruno Weber
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	Mural cells pericyte vasculature calcium signaling Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f6cf4993c732468fb2e6e17c8a3ee4bf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f6cf4993c732468fb2e6e17c8a3ee4bf
record_format	dspace
spelling	oai:doaj.org-article:f6cf4993c732468fb2e6e17c8a3ee4bf2021-12-01T11:00:11ZDistinct signatures of calcium activity in brain mural cells10.7554/eLife.705912050-084Xe70591https://doaj.org/article/f6cf4993c732468fb2e6e17c8a3ee4bf2021-07-01T00:00:00Zhttps://elifesciences.org/articles/70591https://doaj.org/toc/2050-084XPericytes have been implicated in various neuropathologies, yet little is known about their function and signaling pathways in health. Here, we characterized calcium dynamics of cortical mural cells in anesthetized or awake Pdgfrb-CreERT2;Rosa26< LSL-GCaMP6s > mice and in acute brain slices. Smooth muscle cells (SMCs) and ensheathing pericytes (EPs), also named as terminal vascular SMCs, revealed similar calcium dynamics in vivo. In contrast, calcium signals in capillary pericytes (CPs) were irregular, higher in frequency, and occurred in cellular microdomains. In the absence of the vessel constricting agent U46619 in acute slices, SMCs and EPs revealed only sparse calcium signals, whereas CPs retained their spontaneous calcium activity. Interestingly, chemogenetic activation of neurons in vivo and acute elevations of extracellular potassium in brain slices strongly decreased calcium activity in CPs. We propose that neuronal activation and an extracellular increase in potassium suppress calcium activity in CPs, likely mediated by Kir2.2 and KATP channels.Chaim GlückKim David FerrariNoemi BininiAnnika KellerAiman S SaabJillian L StobartBruno WebereLife Sciences Publications LtdarticleMural cellspericytevasculaturecalcium signalingMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mural cells pericyte vasculature calcium signaling Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	Mural cells pericyte vasculature calcium signaling Medicine R Science Q Biology (General) QH301-705.5 Chaim Glück Kim David Ferrari Noemi Binini Annika Keller Aiman S Saab Jillian L Stobart Bruno Weber Distinct signatures of calcium activity in brain mural cells
description	Pericytes have been implicated in various neuropathologies, yet little is known about their function and signaling pathways in health. Here, we characterized calcium dynamics of cortical mural cells in anesthetized or awake Pdgfrb-CreERT2;Rosa26< LSL-GCaMP6s > mice and in acute brain slices. Smooth muscle cells (SMCs) and ensheathing pericytes (EPs), also named as terminal vascular SMCs, revealed similar calcium dynamics in vivo. In contrast, calcium signals in capillary pericytes (CPs) were irregular, higher in frequency, and occurred in cellular microdomains. In the absence of the vessel constricting agent U46619 in acute slices, SMCs and EPs revealed only sparse calcium signals, whereas CPs retained their spontaneous calcium activity. Interestingly, chemogenetic activation of neurons in vivo and acute elevations of extracellular potassium in brain slices strongly decreased calcium activity in CPs. We propose that neuronal activation and an extracellular increase in potassium suppress calcium activity in CPs, likely mediated by Kir2.2 and KATP channels.
format	article
author	Chaim Glück Kim David Ferrari Noemi Binini Annika Keller Aiman S Saab Jillian L Stobart Bruno Weber
author_facet	Chaim Glück Kim David Ferrari Noemi Binini Annika Keller Aiman S Saab Jillian L Stobart Bruno Weber
author_sort	Chaim Glück
title	Distinct signatures of calcium activity in brain mural cells
title_short	Distinct signatures of calcium activity in brain mural cells
title_full	Distinct signatures of calcium activity in brain mural cells
title_fullStr	Distinct signatures of calcium activity in brain mural cells
title_full_unstemmed	Distinct signatures of calcium activity in brain mural cells
title_sort	distinct signatures of calcium activity in brain mural cells
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/f6cf4993c732468fb2e6e17c8a3ee4bf
work_keys_str_mv	AT chaimgluck distinctsignaturesofcalciumactivityinbrainmuralcells AT kimdavidferrari distinctsignaturesofcalciumactivityinbrainmuralcells AT noemibinini distinctsignaturesofcalciumactivityinbrainmuralcells AT annikakeller distinctsignaturesofcalciumactivityinbrainmuralcells AT aimanssaab distinctsignaturesofcalciumactivityinbrainmuralcells AT jillianlstobart distinctsignaturesofcalciumactivityinbrainmuralcells AT brunoweber distinctsignaturesofcalciumactivityinbrainmuralcells
_version_	1718405270389915648

Distinct signatures of calcium activity in brain mural cells

Ejemplares similares