Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria

ABSTRACT Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in co...

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Autores principales:	Phenix-Lan Quan, Cadhla Firth, Craig Street, Jose A. Henriquez, Alexandra Petrosov, Alla Tashmukhamedova, Stephen K. Hutchison, Michael Egholm, Modupe O. V. Osinubi, Michael Niezgoda, Albert B. Ogunkoya, Thomas Briese, Charles E. Rupprecht, W. Ian Lipkin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f6cf8979b0fb4d5d8d39c3e0ff89e1e4
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spelling	oai:doaj.org-article:f6cf8979b0fb4d5d8d39c3e0ff89e1e42021-11-15T15:38:15ZIdentification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria10.1128/mBio.00208-102150-7511https://doaj.org/article/f6cf8979b0fb4d5d8d39c3e0ff89e1e42010-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00208-10https://doaj.org/toc/2150-7511ABSTRACT Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs. IMPORTANCE Bats (order Chiroptera, suborders Megachiroptera and Microchiroptera) are reservoirs for a wide range of viruses that cause diseases in humans and livestock, including the severe acute respiratory syndrome coronavirus (SARS-CoV), responsible for the global SARS outbreak in 2003. The diversity of viruses harbored by bats is only just beginning to be understood because of expanded wildlife surveillance and the development and application of new tools for pathogen discovery. This paper describes a new coronavirus, one with a distinctive genomic organization that may provide insights into coronavirus evolution and biology.Phenix-Lan QuanCadhla FirthCraig StreetJose A. HenriquezAlexandra PetrosovAlla TashmukhamedovaStephen K. HutchisonMichael EgholmModupe O. V. OsinubiMichael NiezgodaAlbert B. OgunkoyaThomas BrieseCharles E. RupprechtW. Ian LipkinAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 1, Iss 4 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Phenix-Lan Quan Cadhla Firth Craig Street Jose A. Henriquez Alexandra Petrosov Alla Tashmukhamedova Stephen K. Hutchison Michael Egholm Modupe O. V. Osinubi Michael Niezgoda Albert B. Ogunkoya Thomas Briese Charles E. Rupprecht W. Ian Lipkin Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
description	ABSTRACT Bats are reservoirs for emerging zoonotic viruses that can have a profound impact on human and animal health, including lyssaviruses, filoviruses, paramyxoviruses, and severe acute respiratory syndrome coronaviruses (SARS-CoVs). In the course of a project focused on pathogen discovery in contexts where human-bat contact might facilitate more efficient interspecies transmission of viruses, we surveyed gastrointestinal tissue obtained from bats collected in caves in Nigeria that are frequented by humans. Coronavirus consensus PCR and unbiased high-throughput pyrosequencing revealed the presence of coronavirus sequences related to those of SARS-CoV in a Commerson's leaf-nosed bat (Hipposideros commersoni). Additional genomic sequencing indicated that this virus, unlike subgroup 2b CoVs, which includes SARS-CoV, is unique, comprising three overlapping open reading frames between the M and N genes and two conserved stem-loop II motifs. Phylogenetic analyses in conjunction with these features suggest that this virus represents a new subgroup within group 2 CoVs. IMPORTANCE Bats (order Chiroptera, suborders Megachiroptera and Microchiroptera) are reservoirs for a wide range of viruses that cause diseases in humans and livestock, including the severe acute respiratory syndrome coronavirus (SARS-CoV), responsible for the global SARS outbreak in 2003. The diversity of viruses harbored by bats is only just beginning to be understood because of expanded wildlife surveillance and the development and application of new tools for pathogen discovery. This paper describes a new coronavirus, one with a distinctive genomic organization that may provide insights into coronavirus evolution and biology.
format	article
author	Phenix-Lan Quan Cadhla Firth Craig Street Jose A. Henriquez Alexandra Petrosov Alla Tashmukhamedova Stephen K. Hutchison Michael Egholm Modupe O. V. Osinubi Michael Niezgoda Albert B. Ogunkoya Thomas Briese Charles E. Rupprecht W. Ian Lipkin
author_facet	Phenix-Lan Quan Cadhla Firth Craig Street Jose A. Henriquez Alexandra Petrosov Alla Tashmukhamedova Stephen K. Hutchison Michael Egholm Modupe O. V. Osinubi Michael Niezgoda Albert B. Ogunkoya Thomas Briese Charles E. Rupprecht W. Ian Lipkin
author_sort	Phenix-Lan Quan
title	Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
title_short	Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
title_full	Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
title_fullStr	Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
title_full_unstemmed	Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria
title_sort	identification of a severe acute respiratory syndrome coronavirus-like virus in a leaf-nosed bat in nigeria
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/f6cf8979b0fb4d5d8d39c3e0ff89e1e4
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Identification of a Severe Acute Respiratory Syndrome Coronavirus-Like Virus in a Leaf-Nosed Bat in Nigeria

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