High temperature-induced production of unreduced pollen and its cytological effects in Populus

Abstract Temperature change is of potential to trigger the formation of unreduced gametes. In this study, we showed that short periods of high temperature treatment can induce the production of 2n pollen in Populus pseudo-simonii Kitag. The meiotic stage, duration of treatment, and temperature have...

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Autores principales:	Jun Wang, Daili Li, Fengnan Shang, Xiangyang Kang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6d38376fdce461fb4eb54f73604bd91
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spelling	oai:doaj.org-article:f6d38376fdce461fb4eb54f73604bd912021-12-02T16:08:12ZHigh temperature-induced production of unreduced pollen and its cytological effects in Populus10.1038/s41598-017-05661-x2045-2322https://doaj.org/article/f6d38376fdce461fb4eb54f73604bd912017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05661-xhttps://doaj.org/toc/2045-2322Abstract Temperature change is of potential to trigger the formation of unreduced gametes. In this study, we showed that short periods of high temperature treatment can induce the production of 2n pollen in Populus pseudo-simonii Kitag. The meiotic stage, duration of treatment, and temperature have significant effects on the induction of 2n pollen. Heat stress resulted in meiotic abnormalities, including failure of chromosome separation, chromosome stickiness, laggards and micronuclei. Spindle disorientations in the second meiotic division, such as parallel, fused, and tripolar spindles, either increased in frequency or were induced de novo by high temperature treatment. We found that the high temperature treatment induced depolymerisation of meiotic microtubular cytoskeleton, resulting in the failure of chromosome segregation. New microtubular cytoskeletons were able to repolymerise in some heat-treated cells after transferring them to normal conditions. However, aberrant cytokinesis occurred owing to defects of new radial microtubule systems, leading to production of monads, dyads, triads, and polyads. This suggested that depolymerisation and incomplete restoration of microtubules may be important for high temperature-induction of unreduced gametes. These findings might help us understand how polyploidisation is induced by temperature-related stress and support the potential effects of global climate change on reproductive development of plants.Jun WangDaili LiFengnan ShangXiangyang KangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Wang Daili Li Fengnan Shang Xiangyang Kang High temperature-induced production of unreduced pollen and its cytological effects in Populus
description	Abstract Temperature change is of potential to trigger the formation of unreduced gametes. In this study, we showed that short periods of high temperature treatment can induce the production of 2n pollen in Populus pseudo-simonii Kitag. The meiotic stage, duration of treatment, and temperature have significant effects on the induction of 2n pollen. Heat stress resulted in meiotic abnormalities, including failure of chromosome separation, chromosome stickiness, laggards and micronuclei. Spindle disorientations in the second meiotic division, such as parallel, fused, and tripolar spindles, either increased in frequency or were induced de novo by high temperature treatment. We found that the high temperature treatment induced depolymerisation of meiotic microtubular cytoskeleton, resulting in the failure of chromosome segregation. New microtubular cytoskeletons were able to repolymerise in some heat-treated cells after transferring them to normal conditions. However, aberrant cytokinesis occurred owing to defects of new radial microtubule systems, leading to production of monads, dyads, triads, and polyads. This suggested that depolymerisation and incomplete restoration of microtubules may be important for high temperature-induction of unreduced gametes. These findings might help us understand how polyploidisation is induced by temperature-related stress and support the potential effects of global climate change on reproductive development of plants.
format	article
author	Jun Wang Daili Li Fengnan Shang Xiangyang Kang
author_facet	Jun Wang Daili Li Fengnan Shang Xiangyang Kang
author_sort	Jun Wang
title	High temperature-induced production of unreduced pollen and its cytological effects in Populus
title_short	High temperature-induced production of unreduced pollen and its cytological effects in Populus
title_full	High temperature-induced production of unreduced pollen and its cytological effects in Populus
title_fullStr	High temperature-induced production of unreduced pollen and its cytological effects in Populus
title_full_unstemmed	High temperature-induced production of unreduced pollen and its cytological effects in Populus
title_sort	high temperature-induced production of unreduced pollen and its cytological effects in populus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f6d38376fdce461fb4eb54f73604bd91
work_keys_str_mv	AT junwang hightemperatureinducedproductionofunreducedpollenanditscytologicaleffectsinpopulus AT dailili hightemperatureinducedproductionofunreducedpollenanditscytologicaleffectsinpopulus AT fengnanshang hightemperatureinducedproductionofunreducedpollenanditscytologicaleffectsinpopulus AT xiangyangkang hightemperatureinducedproductionofunreducedpollenanditscytologicaleffectsinpopulus
_version_	1718384632367415296

High temperature-induced production of unreduced pollen and its cytological effects in Populus

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