Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery

Hong Yuan, Xin Bao, Yong-Zhong Du, Jian You, Fu-Qiang HuCollege of Pharmaceutical Sciences, Zhejiang University, Hangzhou, PR ChinaPurpose: Both polymer micelles and mesoporous silica nanoparticles have been widely researched as vectors for small molecular insoluble drugs. To combine the advantages...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuan H, Bao X, Du YZ, You J, Hu FQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f6d68789fa3341719a3692179ef4f2f6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f6d68789fa3341719a3692179ef4f2f6
record_format	dspace
spelling	oai:doaj.org-article:f6d68789fa3341719a3692179ef4f2f62021-12-02T04:25:22ZPreparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery1176-91141178-2013https://doaj.org/article/f6d68789fa3341719a3692179ef4f2f62012-09-01T00:00:00Zhttp://www.dovepress.com/preparation-and-evaluation-of-sio2-deposited-stearic-acid-g-chitosan-n-a11117https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hong Yuan, Xin Bao, Yong-Zhong Du, Jian You, Fu-Qiang HuCollege of Pharmaceutical Sciences, Zhejiang University, Hangzhou, PR ChinaPurpose: Both polymer micelles and mesoporous silica nanoparticles have been widely researched as vectors for small molecular insoluble drugs. To combine the advantages of copolymers and silica, studies on the preparation of copolymer-silica composites and cellular evaluation were carried out.Methods: First, a stearic acid-g-chitosan (CS-SA) copolymer was synthesized through a coupling reaction, and then silicone oxide (SiO2)-deposited doxorubicin (DOX)-loaded stearic acid-g-chitosan (CS-SA/SiO2/DOX) nanoparticles were prepared through the sol-gel reaction. Physical and chemical properties such as particle size, zeta potential, and morphologies were examined, and small-angle X-ray scattering (SAXS) analysis was employed to identify the mesoporous structures of the generated nanoparticles. Cellular uptake and cytotoxicity studies were also conducted.Results: CS-SA/SiO2/DOX nanoparticles with different amounts of SiO2 deposited were obtained, and SAXS studies showed that mesoporous structures existed in the CS-SA/SiO2/DOX nanoparticles. The mesoporous size of middle-ratio and high-ratio deposited CS-SA/SiO2/DOX nanoparticles were 4&ndash;5 nm and 8&ndash;10 nm, respectively. Based on transmission electron microscopy images of CS-SA/SiO2/DOX nanoparticles, dark rings around the nanoparticles could be observed in contrast with CS-SA/DOX micelles. Furthermore, CS-SA/SiO2/DOX nanoparticles exhibited faster release behavior in vitro than CS-SA/DOX micelles; cellular uptake research in A549 indicated that the CS-SA/SiO2/DOX nanoparticles were taken up by A549 cells more rapidly, and that CS-SA/SiO2/DOX nanoparticles entered the cell more easily when the amount of SiO2 was higher. IC50 values of CS-SA/DOX micelles, CS-SA/SiO2/DOX-4, CS-SA/SiO2/DOX-8, and CS-SA/SiO2/DOX-16 nanoparticles against A549 cells measured using the MTT assay were 1.69, 0.93, 0.32, and 0.12 &micro;g/mL, respectively.Conclusion: SiO2-deposited stearic acid-g-chitosan organic&ndash;inorganic composites show promise as nanocarriers for hydrophobic drugs such as DOX.Keywords: doxorubicin, nanoparticles, SiO2-deposited, stearic acid-g-chitosanYuan HBao XDu YZYou JHu FQDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5119-5128 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yuan H Bao X Du YZ You J Hu FQ Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
description	Hong Yuan, Xin Bao, Yong-Zhong Du, Jian You, Fu-Qiang HuCollege of Pharmaceutical Sciences, Zhejiang University, Hangzhou, PR ChinaPurpose: Both polymer micelles and mesoporous silica nanoparticles have been widely researched as vectors for small molecular insoluble drugs. To combine the advantages of copolymers and silica, studies on the preparation of copolymer-silica composites and cellular evaluation were carried out.Methods: First, a stearic acid-g-chitosan (CS-SA) copolymer was synthesized through a coupling reaction, and then silicone oxide (SiO2)-deposited doxorubicin (DOX)-loaded stearic acid-g-chitosan (CS-SA/SiO2/DOX) nanoparticles were prepared through the sol-gel reaction. Physical and chemical properties such as particle size, zeta potential, and morphologies were examined, and small-angle X-ray scattering (SAXS) analysis was employed to identify the mesoporous structures of the generated nanoparticles. Cellular uptake and cytotoxicity studies were also conducted.Results: CS-SA/SiO2/DOX nanoparticles with different amounts of SiO2 deposited were obtained, and SAXS studies showed that mesoporous structures existed in the CS-SA/SiO2/DOX nanoparticles. The mesoporous size of middle-ratio and high-ratio deposited CS-SA/SiO2/DOX nanoparticles were 4&ndash;5 nm and 8&ndash;10 nm, respectively. Based on transmission electron microscopy images of CS-SA/SiO2/DOX nanoparticles, dark rings around the nanoparticles could be observed in contrast with CS-SA/DOX micelles. Furthermore, CS-SA/SiO2/DOX nanoparticles exhibited faster release behavior in vitro than CS-SA/DOX micelles; cellular uptake research in A549 indicated that the CS-SA/SiO2/DOX nanoparticles were taken up by A549 cells more rapidly, and that CS-SA/SiO2/DOX nanoparticles entered the cell more easily when the amount of SiO2 was higher. IC50 values of CS-SA/DOX micelles, CS-SA/SiO2/DOX-4, CS-SA/SiO2/DOX-8, and CS-SA/SiO2/DOX-16 nanoparticles against A549 cells measured using the MTT assay were 1.69, 0.93, 0.32, and 0.12 &micro;g/mL, respectively.Conclusion: SiO2-deposited stearic acid-g-chitosan organic&ndash;inorganic composites show promise as nanocarriers for hydrophobic drugs such as DOX.Keywords: doxorubicin, nanoparticles, SiO2-deposited, stearic acid-g-chitosan
format	article
author	Yuan H Bao X Du YZ You J Hu FQ
author_facet	Yuan H Bao X Du YZ You J Hu FQ
author_sort	Yuan H
title	Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_short	Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_full	Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_fullStr	Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_full_unstemmed	Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_sort	preparation and evaluation of sio2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/f6d68789fa3341719a3692179ef4f2f6
work_keys_str_mv	AT yuanh preparationandevaluationofsio2depositedstearicacidgchitosannanoparticlesfordoxorubicindelivery AT baox preparationandevaluationofsio2depositedstearicacidgchitosannanoparticlesfordoxorubicindelivery AT duyz preparationandevaluationofsio2depositedstearicacidgchitosannanoparticlesfordoxorubicindelivery AT youj preparationandevaluationofsio2depositedstearicacidgchitosannanoparticlesfordoxorubicindelivery AT hufq preparationandevaluationofsio2depositedstearicacidgchitosannanoparticlesfordoxorubicindelivery
_version_	1718401286392512512

Preparation and evaluation of SiO2-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery

Ejemplares similares