Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.

Commensal bacteria often have an especially rich source of glycan-degrading enzymes which allow them to utilize undigested carbohydrates from the food or the host. The species Ruminococcus gnavus is present in the digestive tract of ≥90% of humans and has been implicated in gut-related diseases such...

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Autores principales:	Emmanuelle H Crost, Louise E Tailford, Gwenaelle Le Gall, Michel Fons, Bernard Henrissat, Nathalie Juge
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6d894b98b704f8fbf268a3e5a2afe04
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spelling	oai:doaj.org-article:f6d894b98b704f8fbf268a3e5a2afe042021-11-18T08:49:29ZUtilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.1932-620310.1371/journal.pone.0076341https://doaj.org/article/f6d894b98b704f8fbf268a3e5a2afe042013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204617/?tool=EBIhttps://doaj.org/toc/1932-6203Commensal bacteria often have an especially rich source of glycan-degrading enzymes which allow them to utilize undigested carbohydrates from the food or the host. The species Ruminococcus gnavus is present in the digestive tract of ≥90% of humans and has been implicated in gut-related diseases such as inflammatory bowel diseases (IBD). Here we analysed the ability of two R. gnavus human strains, E1 and ATCC 29149, to utilize host glycans. We showed that although both strains could assimilate mucin monosaccharides, only R. gnavus ATCC 29149 was able to grow on mucin as a sole carbon source. Comparative genomic analysis of the two R. gnavus strains highlighted potential clusters and glycoside hydrolases (GHs) responsible for the breakdown and utilization of mucin-derived glycans. Transcriptomic and functional activity assays confirmed the importance of specific GH33 sialidase, and GH29 and GH95 fucosidases in the mucin utilisation pathway. Notably, we uncovered a novel pathway by which R. gnavus ATCC 29149 utilises sialic acid from sialylated substrates. Our results also demonstrated the ability of R. gnavus ATCC 29149 to produce propanol and propionate as the end products of metabolism when grown on mucin and fucosylated glycans. These new findings provide molecular insights into the strain-specificity of R. gnavus adaptation to the gut environment advancing our understanding of the role of gut commensals in health and disease.Emmanuelle H CrostLouise E TailfordGwenaelle Le GallMichel FonsBernard HenrissatNathalie JugePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e76341 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emmanuelle H Crost Louise E Tailford Gwenaelle Le Gall Michel Fons Bernard Henrissat Nathalie Juge Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
description	Commensal bacteria often have an especially rich source of glycan-degrading enzymes which allow them to utilize undigested carbohydrates from the food or the host. The species Ruminococcus gnavus is present in the digestive tract of ≥90% of humans and has been implicated in gut-related diseases such as inflammatory bowel diseases (IBD). Here we analysed the ability of two R. gnavus human strains, E1 and ATCC 29149, to utilize host glycans. We showed that although both strains could assimilate mucin monosaccharides, only R. gnavus ATCC 29149 was able to grow on mucin as a sole carbon source. Comparative genomic analysis of the two R. gnavus strains highlighted potential clusters and glycoside hydrolases (GHs) responsible for the breakdown and utilization of mucin-derived glycans. Transcriptomic and functional activity assays confirmed the importance of specific GH33 sialidase, and GH29 and GH95 fucosidases in the mucin utilisation pathway. Notably, we uncovered a novel pathway by which R. gnavus ATCC 29149 utilises sialic acid from sialylated substrates. Our results also demonstrated the ability of R. gnavus ATCC 29149 to produce propanol and propionate as the end products of metabolism when grown on mucin and fucosylated glycans. These new findings provide molecular insights into the strain-specificity of R. gnavus adaptation to the gut environment advancing our understanding of the role of gut commensals in health and disease.
format	article
author	Emmanuelle H Crost Louise E Tailford Gwenaelle Le Gall Michel Fons Bernard Henrissat Nathalie Juge
author_facet	Emmanuelle H Crost Louise E Tailford Gwenaelle Le Gall Michel Fons Bernard Henrissat Nathalie Juge
author_sort	Emmanuelle H Crost
title	Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
title_short	Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
title_full	Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
title_fullStr	Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
title_full_unstemmed	Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.
title_sort	utilisation of mucin glycans by the human gut symbiont ruminococcus gnavus is strain-dependent.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f6d894b98b704f8fbf268a3e5a2afe04
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Utilisation of mucin glycans by the human gut symbiont Ruminococcus gnavus is strain-dependent.

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