A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory

A multi-week laboratory project has been developed to incorporate elements of student investigation, chemotactic behavior of protists, and genetic effects on chemotactic activity of Tetrahymena. The chemotaxis assay is based on spectrophotometric detection of protist-induced light scattering as cel...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rachel Hongo, Robert T. Grammer, Christopher E. Barton
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f6e1fe7a3ca0452c9867c43b46e17a7a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f6e1fe7a3ca0452c9867c43b46e17a7a
record_format	dspace
spelling	oai:doaj.org-article:f6e1fe7a3ca0452c9867c43b46e17a7a2021-11-15T15:04:42ZA Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory10.1128/jmbe.v21i1.18051935-78851935-7877https://doaj.org/article/f6e1fe7a3ca0452c9867c43b46e17a7a2020-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v21i1.1805https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885A multi-week laboratory project has been developed to incorporate elements of student investigation, chemotactic behavior of protists, and genetic effects on chemotactic activity of Tetrahymena. The chemotaxis assay is based on spectrophotometric detection of protist-induced light scattering as cells migrate into a density gradient containing a known attractant. The three-week project consists of an introductory chemotaxis assay, investigation of dose-response effects, and culminates with the exploration of a Tetrahymena genetic mutant with known defects in motility. Additionally, this project incorporates a microscopic investigation of cellular structure and swimming behavior of mutant and wild-type cells. Students have responded well to the nature of the project, displaying clear understanding of the mechanism of the assay as well as the response of the protists to environmental manipulation and the molecular defects in the mutant cells.Rachel HongoRobert T. GrammerChristopher E. BartonAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 21, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Rachel Hongo Robert T. Grammer Christopher E. Barton A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory
description	A multi-week laboratory project has been developed to incorporate elements of student investigation, chemotactic behavior of protists, and genetic effects on chemotactic activity of Tetrahymena. The chemotaxis assay is based on spectrophotometric detection of protist-induced light scattering as cells migrate into a density gradient containing a known attractant. The three-week project consists of an introductory chemotaxis assay, investigation of dose-response effects, and culminates with the exploration of a Tetrahymena genetic mutant with known defects in motility. Additionally, this project incorporates a microscopic investigation of cellular structure and swimming behavior of mutant and wild-type cells. Students have responded well to the nature of the project, displaying clear understanding of the mechanism of the assay as well as the response of the protists to environmental manipulation and the molecular defects in the mutant cells.
format	article
author	Rachel Hongo Robert T. Grammer Christopher E. Barton
author_facet	Rachel Hongo Robert T. Grammer Christopher E. Barton
author_sort	Rachel Hongo
title	A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory
title_short	A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory
title_full	A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory
title_fullStr	A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory
title_full_unstemmed	A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory
title_sort	multiweek project examining the chemotactic behavior of tetrahymena in an undergraduate biology laboratory
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/f6e1fe7a3ca0452c9867c43b46e17a7a
work_keys_str_mv	AT rachelhongo amultiweekprojectexaminingthechemotacticbehavioroftetrahymenainanundergraduatebiologylaboratory AT roberttgrammer amultiweekprojectexaminingthechemotacticbehavioroftetrahymenainanundergraduatebiologylaboratory AT christopherebarton amultiweekprojectexaminingthechemotacticbehavioroftetrahymenainanundergraduatebiologylaboratory AT rachelhongo multiweekprojectexaminingthechemotacticbehavioroftetrahymenainanundergraduatebiologylaboratory AT roberttgrammer multiweekprojectexaminingthechemotacticbehavioroftetrahymenainanundergraduatebiologylaboratory AT christopherebarton multiweekprojectexaminingthechemotacticbehavioroftetrahymenainanundergraduatebiologylaboratory
_version_	1718428228795760640

A Multiweek Project Examining the Chemotactic Behavior of Tetrahymena in an Undergraduate Biology Laboratory

Ejemplares similares