Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages

Intestinal microbiota can affect the intake, storage, and absorption of nutrients in the body, thereby greatly impacting the growth and development of animals. In addition to diet, the breed and growth stages of pigs could also affect changes in the intestinal microbiota. However, research on the de...

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Autores principales:	Chang Lu, Yadan Liu, Yijia Ma, Shu Wang, Chunbo Cai, Yang Yang, Yan Zhao, Guoming Liang, Guoqing Cao, Bugao Li, Sung Woo Kim, Xiaohong Guo, Pengfei Gao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Jinfen White and Mashen piglets 16S rRNA ileum microbiota intestinal microorganism growth stage Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f6e424250af841f88da48d2cffbd8572
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spelling	oai:doaj.org-article:f6e424250af841f88da48d2cffbd85722021-12-02T01:03:31ZComparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages1664-302X10.3389/fmicb.2021.765691https://doaj.org/article/f6e424250af841f88da48d2cffbd85722021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.765691/fullhttps://doaj.org/toc/1664-302XIntestinal microbiota can affect the intake, storage, and absorption of nutrients in the body, thereby greatly impacting the growth and development of animals. In addition to diet, the breed and growth stages of pigs could also affect changes in the intestinal microbiota. However, research on the developmental changes in the ileum microbiota of piglets remains unclear. In this study, the ileum microbiota of Jinfen White and Mashen piglets at different developmental stages were investigated using 16S rRNA sequencing. Physiologically, the villus height of the ileum decreased, and the crypt depth increased during the development of the two pig breeds. Additionally, the serum antioxidant factors in the Jinfen White piglets were significantly higher than in the Mashen piglets at the end of the nursing stage. A total of 690 operational taxonomic units (OTUs) belonging to 21 phyla and 286 genera were identified, of which Firmicutes and Proteobacteria were the dominant phyla during the development of both the Jinfen White and Mashen piglets, accounting for ∼90% of all OTUs. Further research revealed differences in dominant bacteria between the two breeds. With increasing age, the ileum microbial diversity increased, and in both the pig breeds, the proportion of Firmicutes increased, whereas the proportion of Proteobacteria decreased. Additionally, different samples were characterized by specific genera, and different Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were predicted at certain developmental stages. Finally, the correlation between the ileum microbiota and physiological features was analyzed, and it was suggested that the host and environmental factors play important roles in the formation of the microbial community structure in piglets. In summary, we delineated the structure, function, and differences in ileum microbiota between Jinfen White and Mashen piglets during different growth stages. This study helps to understand the development of the intestinal microbiota in local and hybrid pig breeds.Chang LuYadan LiuYijia MaShu WangChunbo CaiYang YangYan ZhaoGuoming LiangGuoqing CaoBugao LiSung Woo KimXiaohong GuoPengfei GaoFrontiers Media S.A.articleJinfen White and Mashen piglets16S rRNAileum microbiotaintestinal microorganismgrowth stageMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Jinfen White and Mashen piglets 16S rRNA ileum microbiota intestinal microorganism growth stage Microbiology QR1-502
spellingShingle	Jinfen White and Mashen piglets 16S rRNA ileum microbiota intestinal microorganism growth stage Microbiology QR1-502 Chang Lu Yadan Liu Yijia Ma Shu Wang Chunbo Cai Yang Yang Yan Zhao Guoming Liang Guoqing Cao Bugao Li Sung Woo Kim Xiaohong Guo Pengfei Gao Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages
description	Intestinal microbiota can affect the intake, storage, and absorption of nutrients in the body, thereby greatly impacting the growth and development of animals. In addition to diet, the breed and growth stages of pigs could also affect changes in the intestinal microbiota. However, research on the developmental changes in the ileum microbiota of piglets remains unclear. In this study, the ileum microbiota of Jinfen White and Mashen piglets at different developmental stages were investigated using 16S rRNA sequencing. Physiologically, the villus height of the ileum decreased, and the crypt depth increased during the development of the two pig breeds. Additionally, the serum antioxidant factors in the Jinfen White piglets were significantly higher than in the Mashen piglets at the end of the nursing stage. A total of 690 operational taxonomic units (OTUs) belonging to 21 phyla and 286 genera were identified, of which Firmicutes and Proteobacteria were the dominant phyla during the development of both the Jinfen White and Mashen piglets, accounting for ∼90% of all OTUs. Further research revealed differences in dominant bacteria between the two breeds. With increasing age, the ileum microbial diversity increased, and in both the pig breeds, the proportion of Firmicutes increased, whereas the proportion of Proteobacteria decreased. Additionally, different samples were characterized by specific genera, and different Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were predicted at certain developmental stages. Finally, the correlation between the ileum microbiota and physiological features was analyzed, and it was suggested that the host and environmental factors play important roles in the formation of the microbial community structure in piglets. In summary, we delineated the structure, function, and differences in ileum microbiota between Jinfen White and Mashen piglets during different growth stages. This study helps to understand the development of the intestinal microbiota in local and hybrid pig breeds.
format	article
author	Chang Lu Yadan Liu Yijia Ma Shu Wang Chunbo Cai Yang Yang Yan Zhao Guoming Liang Guoqing Cao Bugao Li Sung Woo Kim Xiaohong Guo Pengfei Gao
author_facet	Chang Lu Yadan Liu Yijia Ma Shu Wang Chunbo Cai Yang Yang Yan Zhao Guoming Liang Guoqing Cao Bugao Li Sung Woo Kim Xiaohong Guo Pengfei Gao
author_sort	Chang Lu
title	Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages
title_short	Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages
title_full	Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages
title_fullStr	Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages
title_full_unstemmed	Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages
title_sort	comparative evaluation of the ileum microbiota composition in piglets at different growth stages
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f6e424250af841f88da48d2cffbd8572
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Comparative Evaluation of the Ileum Microbiota Composition in Piglets at Different Growth Stages

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