Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.

<h4>Background</h4>Different clonal types of Toxoplasma gondii are thought to be associated with distinct clinical manifestations of infections. Serotyping is a novel technique which may allow to determine the clonal type of T. gondii humans are infected with and to extend typing studies...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pavlo Maksimov, Johannes Zerweck, Aline Maksimov, Andrea Hotop, Uwe Gross, Katrin Spekker, Walter Däubener, Sandra Werdermann, Olaf Niederstrasser, Eckhardt Petri, Marc Mertens, Rainer G Ulrich, Franz J Conraths, Gereon Schares
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6e65c258e754f399cffc18ee3ad12ae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f6e65c258e754f399cffc18ee3ad12ae
record_format	dspace
spelling	oai:doaj.org-article:f6e65c258e754f399cffc18ee3ad12ae2021-11-18T07:24:00ZAnalysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.1932-620310.1371/journal.pone.0034212https://doaj.org/article/f6e65c258e754f399cffc18ee3ad12ae2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22470537/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Different clonal types of Toxoplasma gondii are thought to be associated with distinct clinical manifestations of infections. Serotyping is a novel technique which may allow to determine the clonal type of T. gondii humans are infected with and to extend typing studies to larger populations which include infected but non-diseased individuals.<h4>Methodology</h4>A peptide-microarray test for T. gondii serotyping was established with 54 previously published synthetic peptides, which mimic clonal type-specific epitopes. The test was applied to human sera (n = 174) collected from individuals with an acute T. gondii infection (n = 21), a latent T. gondii infection (n = 53) and from T. gondii-seropositive forest workers (n = 100).<h4>Findings</h4>The majority (n = 124; 71%) of all T. gondii seropositive human sera showed reactions against synthetic peptides with sequences specific for clonal type II (type II peptides). Type I and type III peptides were recognized by 42% (n = 73) or 16% (n = 28) of the human sera, respectively, while type II-III, type I-III or type I-II peptides were recognized by 49% (n = 85), 36% (n = 62) or 14% (n = 25) of the sera, respectively. Highest reaction intensities were observed with synthetic peptides mimicking type II-specific epitopes. A proportion of the sera (n = 22; 13%) showed no reaction with type-specific peptides. Individuals with acute toxoplasmosis reacted with a statistically significantly higher number of peptides as compared to individuals with latent T. gondii infection or seropositive forest workers.<h4>Conclusions</h4>Type II-specific reactions were overrepresented and higher in intensity in the study population, which was in accord with genotyping studies on T. gondii oocysts previously conducted in the same area. There were also individuals with type I- or type III-specific reactions. Well-characterized reference sera and further specific peptide markers are needed to establish and to perform future serotyping approaches with higher resolution.Pavlo MaksimovJohannes ZerweckAline MaksimovAndrea HotopUwe GrossKatrin SpekkerWalter DäubenerSandra WerdermannOlaf NiederstrasserEckhardt PetriMarc MertensRainer G UlrichFranz J ConrathsGereon ScharesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e34212 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pavlo Maksimov Johannes Zerweck Aline Maksimov Andrea Hotop Uwe Gross Katrin Spekker Walter Däubener Sandra Werdermann Olaf Niederstrasser Eckhardt Petri Marc Mertens Rainer G Ulrich Franz J Conraths Gereon Schares Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.
description	<h4>Background</h4>Different clonal types of Toxoplasma gondii are thought to be associated with distinct clinical manifestations of infections. Serotyping is a novel technique which may allow to determine the clonal type of T. gondii humans are infected with and to extend typing studies to larger populations which include infected but non-diseased individuals.<h4>Methodology</h4>A peptide-microarray test for T. gondii serotyping was established with 54 previously published synthetic peptides, which mimic clonal type-specific epitopes. The test was applied to human sera (n = 174) collected from individuals with an acute T. gondii infection (n = 21), a latent T. gondii infection (n = 53) and from T. gondii-seropositive forest workers (n = 100).<h4>Findings</h4>The majority (n = 124; 71%) of all T. gondii seropositive human sera showed reactions against synthetic peptides with sequences specific for clonal type II (type II peptides). Type I and type III peptides were recognized by 42% (n = 73) or 16% (n = 28) of the human sera, respectively, while type II-III, type I-III or type I-II peptides were recognized by 49% (n = 85), 36% (n = 62) or 14% (n = 25) of the sera, respectively. Highest reaction intensities were observed with synthetic peptides mimicking type II-specific epitopes. A proportion of the sera (n = 22; 13%) showed no reaction with type-specific peptides. Individuals with acute toxoplasmosis reacted with a statistically significantly higher number of peptides as compared to individuals with latent T. gondii infection or seropositive forest workers.<h4>Conclusions</h4>Type II-specific reactions were overrepresented and higher in intensity in the study population, which was in accord with genotyping studies on T. gondii oocysts previously conducted in the same area. There were also individuals with type I- or type III-specific reactions. Well-characterized reference sera and further specific peptide markers are needed to establish and to perform future serotyping approaches with higher resolution.
format	article
author	Pavlo Maksimov Johannes Zerweck Aline Maksimov Andrea Hotop Uwe Gross Katrin Spekker Walter Däubener Sandra Werdermann Olaf Niederstrasser Eckhardt Petri Marc Mertens Rainer G Ulrich Franz J Conraths Gereon Schares
author_facet	Pavlo Maksimov Johannes Zerweck Aline Maksimov Andrea Hotop Uwe Gross Katrin Spekker Walter Däubener Sandra Werdermann Olaf Niederstrasser Eckhardt Petri Marc Mertens Rainer G Ulrich Franz J Conraths Gereon Schares
author_sort	Pavlo Maksimov
title	Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.
title_short	Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.
title_full	Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.
title_fullStr	Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.
title_full_unstemmed	Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.
title_sort	analysis of clonal type-specific antibody reactions in toxoplasma gondii seropositive humans from germany by peptide-microarray.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f6e65c258e754f399cffc18ee3ad12ae
work_keys_str_mv	AT pavlomaksimov analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT johanneszerweck analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT alinemaksimov analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT andreahotop analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT uwegross analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT katrinspekker analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT walterdaubener analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT sandrawerdermann analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT olafniederstrasser analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT eckhardtpetri analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT marcmertens analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT rainergulrich analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT franzjconraths analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray AT gereonschares analysisofclonaltypespecificantibodyreactionsintoxoplasmagondiiseropositivehumansfromgermanybypeptidemicroarray
_version_	1718423573138243584

Analysis of clonal type-specific antibody reactions in Toxoplasma gondii seropositive humans from Germany by peptide-microarray.

Ejemplares similares