The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.

An anaerobic three-stage continuous culture model of the human colon (gut model), which represent different anatomical areas of the large intestine, was used to study the effect of S. aureus infection of the gut on the resident faecal microbiota. Studies on the development of the microbiota in the t...

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Autores principales:	Thippeswamy H Sannasiddappa, Adele Costabile, Glenn R Gibson, Simon R Clarke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6e7025723f94dbc911f36dc93932375
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spelling	oai:doaj.org-article:f6e7025723f94dbc911f36dc939323752021-11-18T06:48:24ZThe influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.1932-620310.1371/journal.pone.0023227https://doaj.org/article/f6e7025723f94dbc911f36dc939323752011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21858036/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203An anaerobic three-stage continuous culture model of the human colon (gut model), which represent different anatomical areas of the large intestine, was used to study the effect of S. aureus infection of the gut on the resident faecal microbiota. Studies on the development of the microbiota in the three vessels were performed and bacteria identified by culture independent fluorescence in situ hybridization (FISH). Furthermore, short chain fatty acids (SCFA), as principal end products of gut bacterial metabolism, were measured along with a quantitative assessment of the predominant microbiota. During steady state conditions, numbers of S. aureus cells stabilised until they were washed out, but populations of indigenous bacteria were transiently altered; thus S. aureus was able to compromise colonisation resistance by the colonic microbiota. Furthermore, the concentration of butyric acid in the vessel representing the proximal colon was significantly decreased by infection. Thus infection by S. aureus appears to be able to alter the overall structure of the human colonic microbiota and the microbial metabolic profiles. This work provides an initial in vitro model to analyse interactions with pathogens.Thippeswamy H SannasiddappaAdele CostabileGlenn R GibsonSimon R ClarkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23227 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thippeswamy H Sannasiddappa Adele Costabile Glenn R Gibson Simon R Clarke The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.
description	An anaerobic three-stage continuous culture model of the human colon (gut model), which represent different anatomical areas of the large intestine, was used to study the effect of S. aureus infection of the gut on the resident faecal microbiota. Studies on the development of the microbiota in the three vessels were performed and bacteria identified by culture independent fluorescence in situ hybridization (FISH). Furthermore, short chain fatty acids (SCFA), as principal end products of gut bacterial metabolism, were measured along with a quantitative assessment of the predominant microbiota. During steady state conditions, numbers of S. aureus cells stabilised until they were washed out, but populations of indigenous bacteria were transiently altered; thus S. aureus was able to compromise colonisation resistance by the colonic microbiota. Furthermore, the concentration of butyric acid in the vessel representing the proximal colon was significantly decreased by infection. Thus infection by S. aureus appears to be able to alter the overall structure of the human colonic microbiota and the microbial metabolic profiles. This work provides an initial in vitro model to analyse interactions with pathogens.
format	article
author	Thippeswamy H Sannasiddappa Adele Costabile Glenn R Gibson Simon R Clarke
author_facet	Thippeswamy H Sannasiddappa Adele Costabile Glenn R Gibson Simon R Clarke
author_sort	Thippeswamy H Sannasiddappa
title	The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.
title_short	The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.
title_full	The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.
title_fullStr	The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.
title_full_unstemmed	The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.
title_sort	influence of staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f6e7025723f94dbc911f36dc93932375
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The influence of Staphylococcus aureus on gut microbial ecology in an in vitro continuous culture human colonic model system.

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