Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens

ABSTRACT The use of Tasco (air-dried Ascophyllum nodosum) as a feed supplement for ruminants has been reported to affect rumen fermentation and reduce Escherichia coli O157:H7 shedding in feces, but the mode of action behind this phenomenon is unclear. In this study, the effects of four Tasco levels...

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Autores principales:	Mi Zhou, Martin Hünerberg, Yanhong Chen, Tim Reuter, Tim A. McAllister, Franklin Evans, Alan T. Critchley, Le Luo Guan
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Ascophyllum nodosum Escherichia coli Tasco ram rumen microbiome seaweed Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f6ec53f3afed450b8053935ab3ef8543
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spelling	oai:doaj.org-article:f6ec53f3afed450b8053935ab3ef85432021-11-15T15:22:01ZAir-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens10.1128/mSphere.00017-182379-5042https://doaj.org/article/f6ec53f3afed450b8053935ab3ef85432018-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00017-18https://doaj.org/toc/2379-5042ABSTRACT The use of Tasco (air-dried Ascophyllum nodosum) as a feed supplement for ruminants has been reported to affect rumen fermentation and reduce Escherichia coli O157:H7 shedding in feces, but the mode of action behind this phenomenon is unclear. In this study, the effects of four Tasco levels (0, 1, 3, and 5%) on rumen microbiota and rumen/fecal E. coli O serogroups in rams were investigated. Rumen total bacteria and archaea were linearly reduced (P < 0.001) and protozoa were linearly increased (P < 0.001) by increasing levels of Tasco. The relative abundances of seven bacterial species and one protozoal species differed among Tasco levels. With Tasco, 14 predicted metabolic pathways were enriched while only 3 were suppressed. A lower ruminal butyrate concentration is possibly associated with enrichment of the “butanoate metabolism” pathway in Tasco-fed rams. The ruminal total E. coli population was linearly reduced (P < 0.001) by Tasco. Supplementation with Tasco only completely eliminated O121 in the rumen and feces, and higher levels of Tasco (3 and 5%) reduced fecal shedding of serogroups O45, O103, and O111 even though these serogroups were present in the rumen. Our results suggest that Tasco effectively reduced pathogenic E. coli but had only minimal impacts on rumen fermentation in rams. IMPORTANCE Maintaining product safety and reducing the carbon footprint of production are two sustainability goals of the livestock industry. The objective of this study was to study the impact of Tasco, a product derived from the brown macroalga Ascophyllum nodosum, on the rumen microbiome and its function. The inclusion of Tasco altered both rumen and fecal microbiota levels without affecting rumen fermentation. Tasco reduced fecal Escherichia coli populations and specifically reduced the prevalence of Shiga toxin-producing E. coli O45, O103, O111, and O121 in feces. The findings of this study highlight the application of Tasco as a potential feed additive to reduce pathogen shedding in rams without interfering with ruminal metabolism.Mi ZhouMartin HünerbergYanhong ChenTim ReuterTim A. McAllisterFranklin EvansAlan T. CritchleyLe Luo GuanAmerican Society for MicrobiologyarticleAscophyllum nodosumEscherichia coliTascoramrumen microbiomeseaweedMicrobiologyQR1-502ENmSphere, Vol 3, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ascophyllum nodosum Escherichia coli Tasco ram rumen microbiome seaweed Microbiology QR1-502
spellingShingle	Ascophyllum nodosum Escherichia coli Tasco ram rumen microbiome seaweed Microbiology QR1-502 Mi Zhou Martin Hünerberg Yanhong Chen Tim Reuter Tim A. McAllister Franklin Evans Alan T. Critchley Le Luo Guan Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens
description	ABSTRACT The use of Tasco (air-dried Ascophyllum nodosum) as a feed supplement for ruminants has been reported to affect rumen fermentation and reduce Escherichia coli O157:H7 shedding in feces, but the mode of action behind this phenomenon is unclear. In this study, the effects of four Tasco levels (0, 1, 3, and 5%) on rumen microbiota and rumen/fecal E. coli O serogroups in rams were investigated. Rumen total bacteria and archaea were linearly reduced (P < 0.001) and protozoa were linearly increased (P < 0.001) by increasing levels of Tasco. The relative abundances of seven bacterial species and one protozoal species differed among Tasco levels. With Tasco, 14 predicted metabolic pathways were enriched while only 3 were suppressed. A lower ruminal butyrate concentration is possibly associated with enrichment of the “butanoate metabolism” pathway in Tasco-fed rams. The ruminal total E. coli population was linearly reduced (P < 0.001) by Tasco. Supplementation with Tasco only completely eliminated O121 in the rumen and feces, and higher levels of Tasco (3 and 5%) reduced fecal shedding of serogroups O45, O103, and O111 even though these serogroups were present in the rumen. Our results suggest that Tasco effectively reduced pathogenic E. coli but had only minimal impacts on rumen fermentation in rams. IMPORTANCE Maintaining product safety and reducing the carbon footprint of production are two sustainability goals of the livestock industry. The objective of this study was to study the impact of Tasco, a product derived from the brown macroalga Ascophyllum nodosum, on the rumen microbiome and its function. The inclusion of Tasco altered both rumen and fecal microbiota levels without affecting rumen fermentation. Tasco reduced fecal Escherichia coli populations and specifically reduced the prevalence of Shiga toxin-producing E. coli O45, O103, O111, and O121 in feces. The findings of this study highlight the application of Tasco as a potential feed additive to reduce pathogen shedding in rams without interfering with ruminal metabolism.
format	article
author	Mi Zhou Martin Hünerberg Yanhong Chen Tim Reuter Tim A. McAllister Franklin Evans Alan T. Critchley Le Luo Guan
author_facet	Mi Zhou Martin Hünerberg Yanhong Chen Tim Reuter Tim A. McAllister Franklin Evans Alan T. Critchley Le Luo Guan
author_sort	Mi Zhou
title	Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens
title_short	Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens
title_full	Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens
title_fullStr	Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens
title_full_unstemmed	Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens
title_sort	air-dried brown seaweed, <italic toggle="yes">ascophyllum nodosum</italic>, alters the rumen microbiome in a manner that changes rumen fermentation profiles and lowers the prevalence of foodborne pathogens
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/f6ec53f3afed450b8053935ab3ef8543
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Air-Dried Brown Seaweed, <italic toggle="yes">Ascophyllum nodosum</italic>, Alters the Rumen Microbiome in a Manner That Changes Rumen Fermentation Profiles and Lowers the Prevalence of Foodborne Pathogens

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