Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2

Abstract PET imaging is a widely applicable but a very expensive technology. On-site synthesis is one important contributor to the high cost. In this report, we demonstrated the feasibility of a synthesis-free method for PET imaging of brown adipose tissue (BAT) and translocator protein 18 kDa (TSPO...

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Autores principales:	Jing Yang, Jian Yang, Lu Wang, Anna Moore, Steven H. Liang, Chongzhao Ran
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6ec5eb5636649328d92c44b810d4366
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spelling	oai:doaj.org-article:f6ec5eb5636649328d92c44b810d43662021-12-02T16:06:57ZSynthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl210.1038/s41598-017-09018-22045-2322https://doaj.org/article/f6ec5eb5636649328d92c44b810d43662017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09018-2https://doaj.org/toc/2045-2322Abstract PET imaging is a widely applicable but a very expensive technology. On-site synthesis is one important contributor to the high cost. In this report, we demonstrated the feasibility of a synthesis-free method for PET imaging of brown adipose tissue (BAT) and translocator protein 18 kDa (TSPO) via a combination of disulfiram, an FDA approved drug for alcoholism, and 64CuCl2 (termed 64Cu-Dis). In this method, a step-wise injection protocol of 64CuCl2 and disulfiram was used to accomplish the purpose of synthesis-free. Specifically, disulfiram, an inactive 64Cu ligand, was first injected to allow it to metabolize into diethyldithiocarbamate (DDC), a strong 64Cu ligand, which can chelate 64CuCl2 from the following injection to form the actual PET tracer in situ. Our blocking studies, western blot, and tissue histological imaging suggested that the observed BAT contrast was due to 64Cu-Dis binding to TSPO, which was further confirmed as a specific biomarker for BAT imaging using [18F]-F-DPA, a TSPO-specific PET tracer. Our studies, for the first time, demonstrated that TSPO could serve as a potential imaging biomarker for BAT. We believe that our strategy could be extended to other targets while significantly reducing the cost of PET imaging.Jing YangJian YangLu WangAnna MooreSteven H. LiangChongzhao RanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jing Yang Jian Yang Lu Wang Anna Moore Steven H. Liang Chongzhao Ran Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2
description	Abstract PET imaging is a widely applicable but a very expensive technology. On-site synthesis is one important contributor to the high cost. In this report, we demonstrated the feasibility of a synthesis-free method for PET imaging of brown adipose tissue (BAT) and translocator protein 18 kDa (TSPO) via a combination of disulfiram, an FDA approved drug for alcoholism, and 64CuCl2 (termed 64Cu-Dis). In this method, a step-wise injection protocol of 64CuCl2 and disulfiram was used to accomplish the purpose of synthesis-free. Specifically, disulfiram, an inactive 64Cu ligand, was first injected to allow it to metabolize into diethyldithiocarbamate (DDC), a strong 64Cu ligand, which can chelate 64CuCl2 from the following injection to form the actual PET tracer in situ. Our blocking studies, western blot, and tissue histological imaging suggested that the observed BAT contrast was due to 64Cu-Dis binding to TSPO, which was further confirmed as a specific biomarker for BAT imaging using [18F]-F-DPA, a TSPO-specific PET tracer. Our studies, for the first time, demonstrated that TSPO could serve as a potential imaging biomarker for BAT. We believe that our strategy could be extended to other targets while significantly reducing the cost of PET imaging.
format	article
author	Jing Yang Jian Yang Lu Wang Anna Moore Steven H. Liang Chongzhao Ran
author_facet	Jing Yang Jian Yang Lu Wang Anna Moore Steven H. Liang Chongzhao Ran
author_sort	Jing Yang
title	Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2
title_short	Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2
title_full	Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2
title_fullStr	Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2
title_full_unstemmed	Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2
title_sort	synthesis-free pet imaging of brown adipose tissue and tspo via combination of disulfiram and 64cucl2
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f6ec5eb5636649328d92c44b810d4366
work_keys_str_mv	AT jingyang synthesisfreepetimagingofbrownadiposetissueandtspoviacombinationofdisulfiramand64cucl2 AT jianyang synthesisfreepetimagingofbrownadiposetissueandtspoviacombinationofdisulfiramand64cucl2 AT luwang synthesisfreepetimagingofbrownadiposetissueandtspoviacombinationofdisulfiramand64cucl2 AT annamoore synthesisfreepetimagingofbrownadiposetissueandtspoviacombinationofdisulfiramand64cucl2 AT stevenhliang synthesisfreepetimagingofbrownadiposetissueandtspoviacombinationofdisulfiramand64cucl2 AT chongzhaoran synthesisfreepetimagingofbrownadiposetissueandtspoviacombinationofdisulfiramand64cucl2
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Synthesis-free PET imaging of brown adipose tissue and TSPO via combination of disulfiram and 64CuCl2

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