Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period

The blastogenic response of bovine peripheral blood mononuclear cells (PBMCs) to lipopolysaccharides (LPS) has been investigated for a long time in our laboratories. In particular, a possible correlation between the blastogenic response to LPS and the disease resistance of dairy cows has been sugges...

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Autores principales:	Joel Filipe, Alessia Inglesi, Massimo Amadori, Flavia Guarneri, Laura Menchetti, Giulio Curone, Gabriele Brecchia, Daniele Vigo, Federica Riva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cattle PBMC endotoxin tolerance transition period blastogenic response Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f6f2f0d0aa2c4f768e91d26c5c27e0a4
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spelling	oai:doaj.org-article:f6f2f0d0aa2c4f768e91d26c5c27e0a42021-11-25T17:42:10ZPreliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period10.3390/genes121118012073-4425https://doaj.org/article/f6f2f0d0aa2c4f768e91d26c5c27e0a42021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1801https://doaj.org/toc/2073-4425The blastogenic response of bovine peripheral blood mononuclear cells (PBMCs) to lipopolysaccharides (LPS) has been investigated for a long time in our laboratories. In particular, a possible correlation between the blastogenic response to LPS and the disease resistance of dairy cows has been suggested in previous studies. Isolated PBMCs from eight cows at three different time points during the transition period (T0 = 15 days before calving; T1 = 7 days post-calving; T2 = 21 days post-calving) were cultured in the presence or absence of LPS, and the blastogenic response was assayed 72 h after in vitro stimulation. Moreover, the gene expression of proinflammatory cytokines and kynurenine pathway molecules was investigated by real-time RT-PCR on both unstimulated and stimulated PBMCs. The cows were retrospectively divided into healthy and diseased, based on the development of peripartum diseases (subclinical ketosis and placenta retention). The comparison between healthy and diseased cows suggested that healthy animals seemed to better control the response to LPS. On the contrary, diseased animals showed a much higher inflammatory response to LPS. Moreover, cows were retrospectively classified as high and low responders based on the in vitro proliferative response of PBMCs to LPS, using the median value as a threshold. Unstimulated PBMCs of low responders showed higher expression of the proinflammatory cytokines Interleukin 1-β (IL-1β), Interleukin 6 (IL-6) and Tumor Necrosis Factor-α (TNF-α), compared to high responders. Our preliminary data suggest that, during the peripartum period, high responders seem to be more tolerant to endotoxins and develop a lower inflammatory response to different stressors. Instead, low responders could be more prone to the development of unwanted inflammatory conditions in response to mild/moderate stressors.Joel FilipeAlessia InglesiMassimo AmadoriFlavia GuarneriLaura MenchettiGiulio CuroneGabriele BrecchiaDaniele VigoFederica RivaMDPI AGarticlecattlePBMCendotoxin tolerancetransition periodblastogenic responseGeneticsQH426-470ENGenes, Vol 12, Iss 1801, p 1801 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cattle PBMC endotoxin tolerance transition period blastogenic response Genetics QH426-470
spellingShingle	cattle PBMC endotoxin tolerance transition period blastogenic response Genetics QH426-470 Joel Filipe Alessia Inglesi Massimo Amadori Flavia Guarneri Laura Menchetti Giulio Curone Gabriele Brecchia Daniele Vigo Federica Riva Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period
description	The blastogenic response of bovine peripheral blood mononuclear cells (PBMCs) to lipopolysaccharides (LPS) has been investigated for a long time in our laboratories. In particular, a possible correlation between the blastogenic response to LPS and the disease resistance of dairy cows has been suggested in previous studies. Isolated PBMCs from eight cows at three different time points during the transition period (T0 = 15 days before calving; T1 = 7 days post-calving; T2 = 21 days post-calving) were cultured in the presence or absence of LPS, and the blastogenic response was assayed 72 h after in vitro stimulation. Moreover, the gene expression of proinflammatory cytokines and kynurenine pathway molecules was investigated by real-time RT-PCR on both unstimulated and stimulated PBMCs. The cows were retrospectively divided into healthy and diseased, based on the development of peripartum diseases (subclinical ketosis and placenta retention). The comparison between healthy and diseased cows suggested that healthy animals seemed to better control the response to LPS. On the contrary, diseased animals showed a much higher inflammatory response to LPS. Moreover, cows were retrospectively classified as high and low responders based on the in vitro proliferative response of PBMCs to LPS, using the median value as a threshold. Unstimulated PBMCs of low responders showed higher expression of the proinflammatory cytokines Interleukin 1-β (IL-1β), Interleukin 6 (IL-6) and Tumor Necrosis Factor-α (TNF-α), compared to high responders. Our preliminary data suggest that, during the peripartum period, high responders seem to be more tolerant to endotoxins and develop a lower inflammatory response to different stressors. Instead, low responders could be more prone to the development of unwanted inflammatory conditions in response to mild/moderate stressors.
format	article
author	Joel Filipe Alessia Inglesi Massimo Amadori Flavia Guarneri Laura Menchetti Giulio Curone Gabriele Brecchia Daniele Vigo Federica Riva
author_facet	Joel Filipe Alessia Inglesi Massimo Amadori Flavia Guarneri Laura Menchetti Giulio Curone Gabriele Brecchia Daniele Vigo Federica Riva
author_sort	Joel Filipe
title	Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period
title_short	Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period
title_full	Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period
title_fullStr	Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period
title_full_unstemmed	Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period
title_sort	preliminary evidence of endotoxin tolerance in dairy cows during the transition period
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f6f2f0d0aa2c4f768e91d26c5c27e0a4
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Preliminary Evidence of Endotoxin Tolerance in Dairy Cows during the Transition Period

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