Using recombinant adhesive proteins as durable and green flame-retardant coatings

Current fire retardants are known to be toxic to humans and our environment. As environmental-friendly flame retardants (FRs), protein-based flame retardants have been studied extensively recently, even though they are not durable. In this study, we designed, synthesized and tested a durable protein...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Weng I. Leong, Owen Lok In Lo, Fong Tin Cheng, Wai Man Cheong, Leo Chi U. Seak
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2021
Materias:	Flame retardant protein Cellulose-binding domain (CBD) Mussel foot protein-5 (mfp-5) Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f6f4d7871f2346e09182b405a220b0ce
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f6f4d7871f2346e09182b405a220b0ce
record_format	dspace
spelling	oai:doaj.org-article:f6f4d7871f2346e09182b405a220b0ce2021-11-06T04:33:00ZUsing recombinant adhesive proteins as durable and green flame-retardant coatings2405-805X10.1016/j.synbio.2021.10.005https://doaj.org/article/f6f4d7871f2346e09182b405a220b0ce2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405805X21000685https://doaj.org/toc/2405-805XCurrent fire retardants are known to be toxic to humans and our environment. As environmental-friendly flame retardants (FRs), protein-based flame retardants have been studied extensively recently, even though they are not durable. In this study, we designed, synthesized and tested a durable protein-based FR through the fusion of the adhesion domain from either mussel foot protein-5 (mfp-5) or cellulose-binding domain (CBD) with flame retardant protein (SR protein and alpha casein). We first verified the expression of the recombinant proteins in Escherichia coli using Western blot. Then, we coated the fusion protein (carrying cell lysates) to cotton fabrics and wood and verified with Infrared (IR) spectroscopy. Using a vertical burning test and wood flammability test, we confirmed the flame retardancy of the materials after the protein coating. In the vertical burning test, the SR protein and alpha casein flame retardant proteins with the CBD adhesion domain showed a 50.0% and 43.3% increase in flame retardancy. The data is also consistent in the wood flame retardancy test. Confocal imaging experiments also suggested these new fire retardants can be preserved on the materials well even after washing. Overall, our results showed that flame-retardant proteins with adhesion domains are high potential candidates of green alternative flame retardants.Weng I. LeongOwen Lok In LoFong Tin ChengWai Man CheongLeo Chi U. SeakKeAi Communications Co., Ltd.articleFlame retardant proteinCellulose-binding domain (CBD)Mussel foot protein-5 (mfp-5)BiotechnologyTP248.13-248.65Biology (General)QH301-705.5ENSynthetic and Systems Biotechnology, Vol 6, Iss 4, Pp 369-376 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Flame retardant protein Cellulose-binding domain (CBD) Mussel foot protein-5 (mfp-5) Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
spellingShingle	Flame retardant protein Cellulose-binding domain (CBD) Mussel foot protein-5 (mfp-5) Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Weng I. Leong Owen Lok In Lo Fong Tin Cheng Wai Man Cheong Leo Chi U. Seak Using recombinant adhesive proteins as durable and green flame-retardant coatings
description	Current fire retardants are known to be toxic to humans and our environment. As environmental-friendly flame retardants (FRs), protein-based flame retardants have been studied extensively recently, even though they are not durable. In this study, we designed, synthesized and tested a durable protein-based FR through the fusion of the adhesion domain from either mussel foot protein-5 (mfp-5) or cellulose-binding domain (CBD) with flame retardant protein (SR protein and alpha casein). We first verified the expression of the recombinant proteins in Escherichia coli using Western blot. Then, we coated the fusion protein (carrying cell lysates) to cotton fabrics and wood and verified with Infrared (IR) spectroscopy. Using a vertical burning test and wood flammability test, we confirmed the flame retardancy of the materials after the protein coating. In the vertical burning test, the SR protein and alpha casein flame retardant proteins with the CBD adhesion domain showed a 50.0% and 43.3% increase in flame retardancy. The data is also consistent in the wood flame retardancy test. Confocal imaging experiments also suggested these new fire retardants can be preserved on the materials well even after washing. Overall, our results showed that flame-retardant proteins with adhesion domains are high potential candidates of green alternative flame retardants.
format	article
author	Weng I. Leong Owen Lok In Lo Fong Tin Cheng Wai Man Cheong Leo Chi U. Seak
author_facet	Weng I. Leong Owen Lok In Lo Fong Tin Cheng Wai Man Cheong Leo Chi U. Seak
author_sort	Weng I. Leong
title	Using recombinant adhesive proteins as durable and green flame-retardant coatings
title_short	Using recombinant adhesive proteins as durable and green flame-retardant coatings
title_full	Using recombinant adhesive proteins as durable and green flame-retardant coatings
title_fullStr	Using recombinant adhesive proteins as durable and green flame-retardant coatings
title_full_unstemmed	Using recombinant adhesive proteins as durable and green flame-retardant coatings
title_sort	using recombinant adhesive proteins as durable and green flame-retardant coatings
publisher	KeAi Communications Co., Ltd.
publishDate	2021
url	https://doaj.org/article/f6f4d7871f2346e09182b405a220b0ce
work_keys_str_mv	AT wengileong usingrecombinantadhesiveproteinsasdurableandgreenflameretardantcoatings AT owenlokinlo usingrecombinantadhesiveproteinsasdurableandgreenflameretardantcoatings AT fongtincheng usingrecombinantadhesiveproteinsasdurableandgreenflameretardantcoatings AT waimancheong usingrecombinantadhesiveproteinsasdurableandgreenflameretardantcoatings AT leochiuseak usingrecombinantadhesiveproteinsasdurableandgreenflameretardantcoatings
_version_	1718443895023468544

Using recombinant adhesive proteins as durable and green flame-retardant coatings

Ejemplares similares