Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy

Abstract Interfaces provide the structural basis of essential bone functions. In the hierarchical structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales ranging from nanometers to millimeters, all of which contributing to macroscopic properties. To date, however...

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Autores principales:	Rachel Genthial, Emmanuel Beaurepaire, Marie-Claire Schanne-Klein, Françoise Peyrin, Delphine Farlay, Cécile Olivier, Yohann Bala, Georges Boivin, Jean-Claude Vial, Delphine Débarre, Aurélien Gourrier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6f8a5ad851441909b8bbcb470cd05be
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spelling	oai:doaj.org-article:f6f8a5ad851441909b8bbcb470cd05be2021-12-02T15:06:07ZLabel-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy10.1038/s41598-017-03548-52045-2322https://doaj.org/article/f6f8a5ad851441909b8bbcb470cd05be2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03548-5https://doaj.org/toc/2045-2322Abstract Interfaces provide the structural basis of essential bone functions. In the hierarchical structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales ranging from nanometers to millimeters, all of which contributing to macroscopic properties. To date, however, the complexity or limitations of currently used imaging methods restrict our understanding of this functional integration. Here we address this issue using label-free third-harmonic generation (THG) microscopy. We find that the porous lacuno-canalicular network (LCN), revealing the geometry of osteocytes in the bone matrix, can be directly visualized in 3D with submicron precision over millimetric fields of view compatible with histology. THG also reveals interfaces delineating volumes formed at successive remodeling stages. Finally, we show that the structure of the LCN can be analyzed in relation with that of the extracellular matrix and larger-scale structures by simultaneously recording THG and second-harmonic generation (SHG) signals relating to the collagen organization.Rachel GenthialEmmanuel BeaurepaireMarie-Claire Schanne-KleinFrançoise PeyrinDelphine FarlayCécile OlivierYohann BalaGeorges BoivinJean-Claude VialDelphine DébarreAurélien GourrierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rachel Genthial Emmanuel Beaurepaire Marie-Claire Schanne-Klein Françoise Peyrin Delphine Farlay Cécile Olivier Yohann Bala Georges Boivin Jean-Claude Vial Delphine Débarre Aurélien Gourrier Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
description	Abstract Interfaces provide the structural basis of essential bone functions. In the hierarchical structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales ranging from nanometers to millimeters, all of which contributing to macroscopic properties. To date, however, the complexity or limitations of currently used imaging methods restrict our understanding of this functional integration. Here we address this issue using label-free third-harmonic generation (THG) microscopy. We find that the porous lacuno-canalicular network (LCN), revealing the geometry of osteocytes in the bone matrix, can be directly visualized in 3D with submicron precision over millimetric fields of view compatible with histology. THG also reveals interfaces delineating volumes formed at successive remodeling stages. Finally, we show that the structure of the LCN can be analyzed in relation with that of the extracellular matrix and larger-scale structures by simultaneously recording THG and second-harmonic generation (SHG) signals relating to the collagen organization.
format	article
author	Rachel Genthial Emmanuel Beaurepaire Marie-Claire Schanne-Klein Françoise Peyrin Delphine Farlay Cécile Olivier Yohann Bala Georges Boivin Jean-Claude Vial Delphine Débarre Aurélien Gourrier
author_facet	Rachel Genthial Emmanuel Beaurepaire Marie-Claire Schanne-Klein Françoise Peyrin Delphine Farlay Cécile Olivier Yohann Bala Georges Boivin Jean-Claude Vial Delphine Débarre Aurélien Gourrier
author_sort	Rachel Genthial
title	Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
title_short	Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
title_full	Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
title_fullStr	Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
title_full_unstemmed	Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
title_sort	label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f6f8a5ad851441909b8bbcb470cd05be
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Label-free imaging of bone multiscale porosity and interfaces using third-harmonic generation microscopy

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