Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.

Triatomine assassin bugs comprise hematophagous insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Although the microbiome of these species has been investigated to some extent, only one virus infecting Triatoma infestans has been identified to date. Here, we describe for th...

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Autores principales:	Tarcísio Fontenele de Brito, Vitor Lima Coelho, Maira Arruda Cardoso, Ingrid Alexandre de Abreu Brito, Mateus Antonio Berni, Fides Lea Zenk, Nicola Iovino, Attilio Pane
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f6fae4060ee54798a3fca520f7a16a9a
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spelling	oai:doaj.org-article:f6fae4060ee54798a3fca520f7a16a9a2021-12-02T20:00:23ZTransovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.1553-73661553-737410.1371/journal.ppat.1009780https://doaj.org/article/f6fae4060ee54798a3fca520f7a16a9a2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009780https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Triatomine assassin bugs comprise hematophagous insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Although the microbiome of these species has been investigated to some extent, only one virus infecting Triatoma infestans has been identified to date. Here, we describe for the first time seven (+) single-strand RNA viruses (RpV1-7) infecting Rhodnius prolixus, a primary vector of Chagas disease in Central and South America. We show that the RpVs belong to the Iflaviridae, Permutotetraviridae and Solemoviridae and are vertically transmitted from the mothers to the progeny via transovarial transmission. Consistent with this, all the RpVs, except RpV2 that is related to the entomopathogenic Slow bee paralysis virus, established persistent infections in our R. prolixus colony. Furthermore, we show that R. prolixus ovaries express 22-nucleotide viral siRNAs (vsiRNAs), but not viral piRNAs, that originate from the processing of dsRNA intermediates during viral replication of the RpVs. Interestingly, the permutotetraviruses and sobemoviruses display shared pools of vsiRNAs that might provide the basis for a cross-immunity system. The vsiRNAs are maternally deposited in the eggs, where they likely contribute to reduce the viral load and protect the developing embryos. Our results unveil for the first time a complex core virome in R. prolixus and begin to shed light on the RNAi-based antiviral defenses in triatomines.Tarcísio Fontenele de BritoVitor Lima CoelhoMaira Arruda CardosoIngrid Alexandre de Abreu BritoMateus Antonio BerniFides Lea ZenkNicola IovinoAttilio PanePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 8, p e1009780 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Tarcísio Fontenele de Brito Vitor Lima Coelho Maira Arruda Cardoso Ingrid Alexandre de Abreu Brito Mateus Antonio Berni Fides Lea Zenk Nicola Iovino Attilio Pane Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.
description	Triatomine assassin bugs comprise hematophagous insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Although the microbiome of these species has been investigated to some extent, only one virus infecting Triatoma infestans has been identified to date. Here, we describe for the first time seven (+) single-strand RNA viruses (RpV1-7) infecting Rhodnius prolixus, a primary vector of Chagas disease in Central and South America. We show that the RpVs belong to the Iflaviridae, Permutotetraviridae and Solemoviridae and are vertically transmitted from the mothers to the progeny via transovarial transmission. Consistent with this, all the RpVs, except RpV2 that is related to the entomopathogenic Slow bee paralysis virus, established persistent infections in our R. prolixus colony. Furthermore, we show that R. prolixus ovaries express 22-nucleotide viral siRNAs (vsiRNAs), but not viral piRNAs, that originate from the processing of dsRNA intermediates during viral replication of the RpVs. Interestingly, the permutotetraviruses and sobemoviruses display shared pools of vsiRNAs that might provide the basis for a cross-immunity system. The vsiRNAs are maternally deposited in the eggs, where they likely contribute to reduce the viral load and protect the developing embryos. Our results unveil for the first time a complex core virome in R. prolixus and begin to shed light on the RNAi-based antiviral defenses in triatomines.
format	article
author	Tarcísio Fontenele de Brito Vitor Lima Coelho Maira Arruda Cardoso Ingrid Alexandre de Abreu Brito Mateus Antonio Berni Fides Lea Zenk Nicola Iovino Attilio Pane
author_facet	Tarcísio Fontenele de Brito Vitor Lima Coelho Maira Arruda Cardoso Ingrid Alexandre de Abreu Brito Mateus Antonio Berni Fides Lea Zenk Nicola Iovino Attilio Pane
author_sort	Tarcísio Fontenele de Brito
title	Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.
title_short	Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.
title_full	Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.
title_fullStr	Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.
title_full_unstemmed	Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.
title_sort	transovarial transmission of a core virome in the chagas disease vector rhodnius prolixus.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f6fae4060ee54798a3fca520f7a16a9a
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Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.

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