Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models

Summary: Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) with resistance to neutralizing antibodies are threatening to undermine vaccine efficacy. Vaccination and infection have led to widespread humoral immunity against the pandemic founder (Wu-Hu-1). Against...

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Autores principales:	Daniel J. Sheward, Marco Mandolesi, Egon Urgard, Changil Kim, Leo Hanke, Laura Perez Vidakovics, Alec Pankow, Natalie L. Smith, Xaquin Castro Dopico, Gerald M. McInerney, Jonathan M. Coquet, Gunilla B. Karlsson Hedestam, Ben Murrell
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	SARS-CoV-2 variants of concern vaccines original antigenic sin heterotypic boost passive immunization Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f6fbb94e1aa445b28a40632a0756d791
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spelling	oai:doaj.org-article:f6fbb94e1aa445b28a40632a0756d7912021-11-18T04:52:17ZBeta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models2666-379110.1016/j.xcrm.2021.100450https://doaj.org/article/f6fbb94e1aa445b28a40632a0756d7912021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666379121003189https://doaj.org/toc/2666-3791Summary: Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) with resistance to neutralizing antibodies are threatening to undermine vaccine efficacy. Vaccination and infection have led to widespread humoral immunity against the pandemic founder (Wu-Hu-1). Against this background, it is critical to assess the outcomes of subsequent immunization with variant antigens. It is not yet clear whether heterotypic boosts would be compromised by original antigenic sin, where pre-existing responses to a prior variant dampen responses to a new one, or whether the memory B cell repertoire would bridge the gap between Wu-Hu-1 and VOCs. We show, in macaques immunized with Wu-Hu-1 spike, that a single dose of adjuvanted beta variant receptor binding domain (RBD) protein broadens neutralizing antibody responses to heterologous VOCs. Passive transfer of plasma sampled after Wu-Hu-1 spike immunization only partially protects K18-hACE2 mice from lethal challenge with a beta variant isolate, whereas plasma sampled following heterotypic RBD boost protects completely against disease.Daniel J. ShewardMarco MandolesiEgon UrgardChangil KimLeo HankeLaura Perez VidakovicsAlec PankowNatalie L. SmithXaquin Castro DopicoGerald M. McInerneyJonathan M. CoquetGunilla B. Karlsson HedestamBen MurrellElsevierarticleSARS-CoV-2variants of concernvaccinesoriginal antigenic sinheterotypic boostpassive immunizationMedicine (General)R5-920ENCell Reports Medicine, Vol 2, Iss 11, Pp 100450- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 variants of concern vaccines original antigenic sin heterotypic boost passive immunization Medicine (General) R5-920
spellingShingle	SARS-CoV-2 variants of concern vaccines original antigenic sin heterotypic boost passive immunization Medicine (General) R5-920 Daniel J. Sheward Marco Mandolesi Egon Urgard Changil Kim Leo Hanke Laura Perez Vidakovics Alec Pankow Natalie L. Smith Xaquin Castro Dopico Gerald M. McInerney Jonathan M. Coquet Gunilla B. Karlsson Hedestam Ben Murrell Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models
description	Summary: Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) with resistance to neutralizing antibodies are threatening to undermine vaccine efficacy. Vaccination and infection have led to widespread humoral immunity against the pandemic founder (Wu-Hu-1). Against this background, it is critical to assess the outcomes of subsequent immunization with variant antigens. It is not yet clear whether heterotypic boosts would be compromised by original antigenic sin, where pre-existing responses to a prior variant dampen responses to a new one, or whether the memory B cell repertoire would bridge the gap between Wu-Hu-1 and VOCs. We show, in macaques immunized with Wu-Hu-1 spike, that a single dose of adjuvanted beta variant receptor binding domain (RBD) protein broadens neutralizing antibody responses to heterologous VOCs. Passive transfer of plasma sampled after Wu-Hu-1 spike immunization only partially protects K18-hACE2 mice from lethal challenge with a beta variant isolate, whereas plasma sampled following heterotypic RBD boost protects completely against disease.
format	article
author	Daniel J. Sheward Marco Mandolesi Egon Urgard Changil Kim Leo Hanke Laura Perez Vidakovics Alec Pankow Natalie L. Smith Xaquin Castro Dopico Gerald M. McInerney Jonathan M. Coquet Gunilla B. Karlsson Hedestam Ben Murrell
author_facet	Daniel J. Sheward Marco Mandolesi Egon Urgard Changil Kim Leo Hanke Laura Perez Vidakovics Alec Pankow Natalie L. Smith Xaquin Castro Dopico Gerald M. McInerney Jonathan M. Coquet Gunilla B. Karlsson Hedestam Ben Murrell
author_sort	Daniel J. Sheward
title	Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models
title_short	Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models
title_full	Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models
title_fullStr	Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models
title_full_unstemmed	Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models
title_sort	beta rbd boost broadens antibody-mediated protection against sars-cov-2 variants in animal models
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f6fbb94e1aa445b28a40632a0756d791
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Beta RBD boost broadens antibody-mediated protection against SARS-CoV-2 variants in animal models

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