Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.

Double minute chromosomes or double minutes (DMs) are cytogenetic hallmarks of extrachromosomal genomic amplification and play a critical role in tumorigenesis. Amplified copies of oncogenes in DMs have been associated with increased growth and survival of cancer cells but DNA sequences in DMs which...

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Autores principales:	Yan Jin, Zheng Liu, Wei Cao, Xinying Ma, Yihui Fan, Yang Yu, Jing Bai, Feng Chen, Jesusa Rosales, Ki-Young Lee, Songbin Fu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6fe723c14bf45098a2e9811c92dac7d
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spelling	oai:doaj.org-article:f6fe723c14bf45098a2e9811c92dac7d2021-11-18T07:28:57ZNovel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.1932-620310.1371/journal.pone.0030419https://doaj.org/article/f6fe723c14bf45098a2e9811c92dac7d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22319568/?tool=EBIhttps://doaj.org/toc/1932-6203Double minute chromosomes or double minutes (DMs) are cytogenetic hallmarks of extrachromosomal genomic amplification and play a critical role in tumorigenesis. Amplified copies of oncogenes in DMs have been associated with increased growth and survival of cancer cells but DNA sequences in DMs which are mostly non-coding remain to be characterized. Following sequencing and bioinformatics analyses, we have found 5 novel matrix attachment regions (MARs) in a 682 kb DM in the human ovarian cancer cell line, UACC-1598. By electrophoretic mobility shift assay (EMSA), we determined that all 5 MARs interact with the nuclear matrix in vitro. Furthermore, qPCR analysis revealed that these MARs associate with the nuclear matrix in vivo, indicating that they are functional. Transfection of MARs constructs into human embryonic kidney 293T cells showed significant enhancement of gene expression as measured by luciferase assay, suggesting that the identified MARS, particularly MARs 1 to 4, regulate their target genes in vivo and are potentially involved in DM-mediated oncogene activation.Yan JinZheng LiuWei CaoXinying MaYihui FanYang YuJing BaiFeng ChenJesusa RosalesKi-Young LeeSongbin FuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e30419 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Jin Zheng Liu Wei Cao Xinying Ma Yihui Fan Yang Yu Jing Bai Feng Chen Jesusa Rosales Ki-Young Lee Songbin Fu Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
description	Double minute chromosomes or double minutes (DMs) are cytogenetic hallmarks of extrachromosomal genomic amplification and play a critical role in tumorigenesis. Amplified copies of oncogenes in DMs have been associated with increased growth and survival of cancer cells but DNA sequences in DMs which are mostly non-coding remain to be characterized. Following sequencing and bioinformatics analyses, we have found 5 novel matrix attachment regions (MARs) in a 682 kb DM in the human ovarian cancer cell line, UACC-1598. By electrophoretic mobility shift assay (EMSA), we determined that all 5 MARs interact with the nuclear matrix in vitro. Furthermore, qPCR analysis revealed that these MARs associate with the nuclear matrix in vivo, indicating that they are functional. Transfection of MARs constructs into human embryonic kidney 293T cells showed significant enhancement of gene expression as measured by luciferase assay, suggesting that the identified MARS, particularly MARs 1 to 4, regulate their target genes in vivo and are potentially involved in DM-mediated oncogene activation.
format	article
author	Yan Jin Zheng Liu Wei Cao Xinying Ma Yihui Fan Yang Yu Jing Bai Feng Chen Jesusa Rosales Ki-Young Lee Songbin Fu
author_facet	Yan Jin Zheng Liu Wei Cao Xinying Ma Yihui Fan Yang Yu Jing Bai Feng Chen Jesusa Rosales Ki-Young Lee Songbin Fu
author_sort	Yan Jin
title	Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
title_short	Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
title_full	Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
title_fullStr	Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
title_full_unstemmed	Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
title_sort	novel functional mar elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f6fe723c14bf45098a2e9811c92dac7d
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Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression.

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