General cell-binding activity of intramolecular G-quadruplexes with parallel structure.

G-quadruplexes (G4s) are four-stranded nucleic acid structures adopted by some repetitive guanine-rich sequences. Putative G-quadruplex-forming sequences (PQSs) are highly prevalent in human genome. Recently some G4s have been reported to have cancer-selective antiproliferative activity. A G4 DNA, A...

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Autores principales:	Tianjun Chang, Cui Qi, Jie Meng, Nan Zhang, Tao Bing, Xianda Yang, Zehui Cao, Dihua Shangguan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f6ffdc195273491a86bdfe54e335cdbb
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spelling	oai:doaj.org-article:f6ffdc195273491a86bdfe54e335cdbb2021-11-18T07:47:44ZGeneral cell-binding activity of intramolecular G-quadruplexes with parallel structure.1932-620310.1371/journal.pone.0062348https://doaj.org/article/f6ffdc195273491a86bdfe54e335cdbb2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23638046/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203G-quadruplexes (G4s) are four-stranded nucleic acid structures adopted by some repetitive guanine-rich sequences. Putative G-quadruplex-forming sequences (PQSs) are highly prevalent in human genome. Recently some G4s have been reported to have cancer-selective antiproliferative activity. A G4 DNA, AS1411, is currently in phase II clinical trials as an anticancer agent, which is reported to bind tumor cells by targeting surface nucleolin. AS1411 also has been extensively investigated as a target-recognition element for cancer cell specific drug delivery or cancer cell imaging. Here we show that, in addition to AS1411, intramolecular G4s with parallel structure (including PQSs in genes) have general binding activity to many cell lines with different affinity. The binding of these G4s compete with each other, and their targets are certain cellular surface proteins. The tested G4s exhibit enhanced cellular uptake than non-G4 sequences. This uptake may be through the endosome/lysosome pathway, but it is independent of cellular binding of the G4s. The tested G4s also show selective antiproliferative activity that is independent of their cellular binding. Our findings provide new insight into the molecular recognition of G4s by cells; offer new clues for understanding the functions of G4s in vivo, and may extend the potential applications of G4s.Tianjun ChangCui QiJie MengNan ZhangTao BingXianda YangZehui CaoDihua ShangguanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62348 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tianjun Chang Cui Qi Jie Meng Nan Zhang Tao Bing Xianda Yang Zehui Cao Dihua Shangguan General cell-binding activity of intramolecular G-quadruplexes with parallel structure.
description	G-quadruplexes (G4s) are four-stranded nucleic acid structures adopted by some repetitive guanine-rich sequences. Putative G-quadruplex-forming sequences (PQSs) are highly prevalent in human genome. Recently some G4s have been reported to have cancer-selective antiproliferative activity. A G4 DNA, AS1411, is currently in phase II clinical trials as an anticancer agent, which is reported to bind tumor cells by targeting surface nucleolin. AS1411 also has been extensively investigated as a target-recognition element for cancer cell specific drug delivery or cancer cell imaging. Here we show that, in addition to AS1411, intramolecular G4s with parallel structure (including PQSs in genes) have general binding activity to many cell lines with different affinity. The binding of these G4s compete with each other, and their targets are certain cellular surface proteins. The tested G4s exhibit enhanced cellular uptake than non-G4 sequences. This uptake may be through the endosome/lysosome pathway, but it is independent of cellular binding of the G4s. The tested G4s also show selective antiproliferative activity that is independent of their cellular binding. Our findings provide new insight into the molecular recognition of G4s by cells; offer new clues for understanding the functions of G4s in vivo, and may extend the potential applications of G4s.
format	article
author	Tianjun Chang Cui Qi Jie Meng Nan Zhang Tao Bing Xianda Yang Zehui Cao Dihua Shangguan
author_facet	Tianjun Chang Cui Qi Jie Meng Nan Zhang Tao Bing Xianda Yang Zehui Cao Dihua Shangguan
author_sort	Tianjun Chang
title	General cell-binding activity of intramolecular G-quadruplexes with parallel structure.
title_short	General cell-binding activity of intramolecular G-quadruplexes with parallel structure.
title_full	General cell-binding activity of intramolecular G-quadruplexes with parallel structure.
title_fullStr	General cell-binding activity of intramolecular G-quadruplexes with parallel structure.
title_full_unstemmed	General cell-binding activity of intramolecular G-quadruplexes with parallel structure.
title_sort	general cell-binding activity of intramolecular g-quadruplexes with parallel structure.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f6ffdc195273491a86bdfe54e335cdbb
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General cell-binding activity of intramolecular G-quadruplexes with parallel structure.

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