An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.

Mammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had been made in understanding the physiology and biochemistry of capacitation, little is known about the potential roles of epididymal proteins during this process. Here we report that HongrES1, a new member...

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Autores principales:	Yuchuan Zhou, Min Zheng, Qixian Shi, Li Zhang, Wei Zhen, Wenying Chen, Yonglian Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f701d6804e6e4e42ac8e594cee9683b5
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spelling	oai:doaj.org-article:f701d6804e6e4e42ac8e594cee9683b52021-11-25T06:17:55ZAn epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.1932-620310.1371/journal.pone.0004106https://doaj.org/article/f701d6804e6e4e42ac8e594cee9683b52008-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19116669/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had been made in understanding the physiology and biochemistry of capacitation, little is known about the potential roles of epididymal proteins during this process. Here we report that HongrES1, a new member of the SERPIN (serine proteinase inhibitor) family exclusively expressed in the rat cauda epididymis and up-regulated by androgen, is secreted into the lumen and covers the sperm head. Co-culture of caudal sperms with HongrES1 antibody in vitro resulted in a significant increase in the percentage of capacitated spermatozoa. Furthermore, the percentage of capacitated spermatozoa clearly increased in rats when HongrES1 was down-regulated by RNAi in vivo. Remarkably, knockdown of HongrES1 in vivo led to reduced fertility accompanied with deformed appearance of fetuses and pups. These results identify HongrES1 as a novel and critical molecule in the regulation of sperm capacitation and male fertility.Yuchuan ZhouMin ZhengQixian ShiLi ZhangWei ZhenWenying ChenYonglian ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 12, p e4106 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuchuan Zhou Min Zheng Qixian Shi Li Zhang Wei Zhen Wenying Chen Yonglian Zhang An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
description	Mammalian sperm capacitation is an essential prerequisite to fertilizion. Although progress had been made in understanding the physiology and biochemistry of capacitation, little is known about the potential roles of epididymal proteins during this process. Here we report that HongrES1, a new member of the SERPIN (serine proteinase inhibitor) family exclusively expressed in the rat cauda epididymis and up-regulated by androgen, is secreted into the lumen and covers the sperm head. Co-culture of caudal sperms with HongrES1 antibody in vitro resulted in a significant increase in the percentage of capacitated spermatozoa. Furthermore, the percentage of capacitated spermatozoa clearly increased in rats when HongrES1 was down-regulated by RNAi in vivo. Remarkably, knockdown of HongrES1 in vivo led to reduced fertility accompanied with deformed appearance of fetuses and pups. These results identify HongrES1 as a novel and critical molecule in the regulation of sperm capacitation and male fertility.
format	article
author	Yuchuan Zhou Min Zheng Qixian Shi Li Zhang Wei Zhen Wenying Chen Yonglian Zhang
author_facet	Yuchuan Zhou Min Zheng Qixian Shi Li Zhang Wei Zhen Wenying Chen Yonglian Zhang
author_sort	Yuchuan Zhou
title	An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_short	An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_full	An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_fullStr	An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_full_unstemmed	An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.
title_sort	epididymis-specific secretory protein hongres1 critically regulates sperm capacitation and male fertility.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/f701d6804e6e4e42ac8e594cee9683b5
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An epididymis-specific secretory protein HongrES1 critically regulates sperm capacitation and male fertility.

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