Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage

Abstract In mammalian ovaries, mitochondria are integral sites of energy production and steroidogenesis. While shifts in cellular activities and steroidogenesis are well characterized during the differentiation of large luteal cells in folliculogenesis and luteal formation, mitochondrial dynamics du...

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Autores principales:	Yi-Fan Jiang, Pin-Huan Yu, Yovita Permata Budi, Chih-Hsien Chiu, Chi-Yu Fu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7158d9afa7e4fcc8771aec10b9362b1
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spelling	oai:doaj.org-article:f7158d9afa7e4fcc8771aec10b9362b12021-12-02T16:24:56ZDynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage10.1038/s41598-021-95161-w2045-2322https://doaj.org/article/f7158d9afa7e4fcc8771aec10b9362b12021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95161-whttps://doaj.org/toc/2045-2322Abstract In mammalian ovaries, mitochondria are integral sites of energy production and steroidogenesis. While shifts in cellular activities and steroidogenesis are well characterized during the differentiation of large luteal cells in folliculogenesis and luteal formation, mitochondrial dynamics during this process have not been previously evaluated. In this study, we collected ovaries containing primordial follicles, mature follicles, corpus hemorrhagicum, or corpus luteum from goats at specific times in the estrous cycle. Enzyme histochemistry, ultrastructural observations, and 3D structural analysis of serial sections of mitochondria revealed that branched mitochondrial networks were predominant in follicles, while spherical and tubular mitochondria were typical in large luteal cells. Furthermore, the average mitochondrial diameter and volume increased from folliculogenesis to luteal formation. In primordial follicles, the signals of cytochrome c oxidase and ATP synthase were undetectable in most cells, and the large luteal cells from the corpus hemorrhagicum also showed low enzyme signals and content when compared with granulosa cells in mature follicles or large luteal cells from the corpus luteum. Our findings suggest that the mitochondrial enlargement could be an event during folliculogenesis and luteal formation, while the modulation of mitochondrial morphology and respiratory enzyme expressions may be related to tissue remodeling during luteal formation.Yi-Fan JiangPin-Huan YuYovita Permata BudiChih-Hsien ChiuChi-Yu FuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yi-Fan Jiang Pin-Huan Yu Yovita Permata Budi Chih-Hsien Chiu Chi-Yu Fu Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage
description	Abstract In mammalian ovaries, mitochondria are integral sites of energy production and steroidogenesis. While shifts in cellular activities and steroidogenesis are well characterized during the differentiation of large luteal cells in folliculogenesis and luteal formation, mitochondrial dynamics during this process have not been previously evaluated. In this study, we collected ovaries containing primordial follicles, mature follicles, corpus hemorrhagicum, or corpus luteum from goats at specific times in the estrous cycle. Enzyme histochemistry, ultrastructural observations, and 3D structural analysis of serial sections of mitochondria revealed that branched mitochondrial networks were predominant in follicles, while spherical and tubular mitochondria were typical in large luteal cells. Furthermore, the average mitochondrial diameter and volume increased from folliculogenesis to luteal formation. In primordial follicles, the signals of cytochrome c oxidase and ATP synthase were undetectable in most cells, and the large luteal cells from the corpus hemorrhagicum also showed low enzyme signals and content when compared with granulosa cells in mature follicles or large luteal cells from the corpus luteum. Our findings suggest that the mitochondrial enlargement could be an event during folliculogenesis and luteal formation, while the modulation of mitochondrial morphology and respiratory enzyme expressions may be related to tissue remodeling during luteal formation.
format	article
author	Yi-Fan Jiang Pin-Huan Yu Yovita Permata Budi Chih-Hsien Chiu Chi-Yu Fu
author_facet	Yi-Fan Jiang Pin-Huan Yu Yovita Permata Budi Chih-Hsien Chiu Chi-Yu Fu
author_sort	Yi-Fan Jiang
title	Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage
title_short	Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage
title_full	Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage
title_fullStr	Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage
title_full_unstemmed	Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage
title_sort	dynamic changes in mitochondrial 3d structure during folliculogenesis and luteal formation in the goat large luteal cell lineage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f7158d9afa7e4fcc8771aec10b9362b1
work_keys_str_mv	AT yifanjiang dynamicchangesinmitochondrial3dstructureduringfolliculogenesisandlutealformationinthegoatlargelutealcelllineage AT pinhuanyu dynamicchangesinmitochondrial3dstructureduringfolliculogenesisandlutealformationinthegoatlargelutealcelllineage AT yovitapermatabudi dynamicchangesinmitochondrial3dstructureduringfolliculogenesisandlutealformationinthegoatlargelutealcelllineage AT chihhsienchiu dynamicchangesinmitochondrial3dstructureduringfolliculogenesisandlutealformationinthegoatlargelutealcelllineage AT chiyufu dynamicchangesinmitochondrial3dstructureduringfolliculogenesisandlutealformationinthegoatlargelutealcelllineage
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Dynamic changes in mitochondrial 3D structure during folliculogenesis and luteal formation in the goat large luteal cell lineage

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