Changes in the gene expression profile during spontaneous migraine attacks

Abstract Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which...

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Autores principales:	Lisette J. A. Kogelman, Katrine Falkenberg, Alfonso Buil, Pau Erola, Julie Courraud, Susan Svane Laursen, Tom Michoel, Jes Olesen, Thomas F. Hansen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f715f3d6980b4cd2a3675cf887e67e77
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spelling	oai:doaj.org-article:f715f3d6980b4cd2a3675cf887e67e772021-12-02T15:51:14ZChanges in the gene expression profile during spontaneous migraine attacks10.1038/s41598-021-87503-52045-2322https://doaj.org/article/f715f3d6980b4cd2a3675cf887e67e772021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87503-5https://doaj.org/toc/2045-2322Abstract Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which are specific to migraine. Twenty-seven migraine patients were assessed during a spontaneous migraine attack, including headache characteristics and treatment effect. Blood samples were taken during attack, two hours after treatment, on a headache-free day and after a cold pressor test. RNA-Sequencing, genotyping, and steroid profiling were performed. RNA-Sequences were analyzed at gene level (differential expression analysis) and at network level, and genomic and transcriptomic data were integrated. We found 29 differentially expressed genes between ‘attack’ and ‘after treatment’, after subtracting non-migraine specific genes, that were functioning in fatty acid oxidation, signaling pathways and immune-related pathways. Network analysis revealed mechanisms affected by changes in gene interactions, e.g. ‘ion transmembrane transport’. Integration of genomic and transcriptomic data revealed pathways related to sumatriptan treatment, i.e. ‘5HT1 type receptor mediated signaling pathway’. In conclusion, we uniquely investigated intra-individual changes in gene expression during a migraine attack. We revealed both genes and pathways potentially involved in the pathophysiology of migraine and/or migraine treatment.Lisette J. A. KogelmanKatrine FalkenbergAlfonso BuilPau ErolaJulie CourraudSusan Svane LaursenTom MichoelJes OlesenThomas F. HansenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisette J. A. Kogelman Katrine Falkenberg Alfonso Buil Pau Erola Julie Courraud Susan Svane Laursen Tom Michoel Jes Olesen Thomas F. Hansen Changes in the gene expression profile during spontaneous migraine attacks
description	Abstract Migraine attacks are delimited, allowing investigation of changes during and outside attack. Gene expression fluctuates according to environmental and endogenous events and therefore, we hypothesized that changes in RNA expression during and outside a spontaneous migraine attack exist which are specific to migraine. Twenty-seven migraine patients were assessed during a spontaneous migraine attack, including headache characteristics and treatment effect. Blood samples were taken during attack, two hours after treatment, on a headache-free day and after a cold pressor test. RNA-Sequencing, genotyping, and steroid profiling were performed. RNA-Sequences were analyzed at gene level (differential expression analysis) and at network level, and genomic and transcriptomic data were integrated. We found 29 differentially expressed genes between ‘attack’ and ‘after treatment’, after subtracting non-migraine specific genes, that were functioning in fatty acid oxidation, signaling pathways and immune-related pathways. Network analysis revealed mechanisms affected by changes in gene interactions, e.g. ‘ion transmembrane transport’. Integration of genomic and transcriptomic data revealed pathways related to sumatriptan treatment, i.e. ‘5HT1 type receptor mediated signaling pathway’. In conclusion, we uniquely investigated intra-individual changes in gene expression during a migraine attack. We revealed both genes and pathways potentially involved in the pathophysiology of migraine and/or migraine treatment.
format	article
author	Lisette J. A. Kogelman Katrine Falkenberg Alfonso Buil Pau Erola Julie Courraud Susan Svane Laursen Tom Michoel Jes Olesen Thomas F. Hansen
author_facet	Lisette J. A. Kogelman Katrine Falkenberg Alfonso Buil Pau Erola Julie Courraud Susan Svane Laursen Tom Michoel Jes Olesen Thomas F. Hansen
author_sort	Lisette J. A. Kogelman
title	Changes in the gene expression profile during spontaneous migraine attacks
title_short	Changes in the gene expression profile during spontaneous migraine attacks
title_full	Changes in the gene expression profile during spontaneous migraine attacks
title_fullStr	Changes in the gene expression profile during spontaneous migraine attacks
title_full_unstemmed	Changes in the gene expression profile during spontaneous migraine attacks
title_sort	changes in the gene expression profile during spontaneous migraine attacks
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f715f3d6980b4cd2a3675cf887e67e77
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Changes in the gene expression profile during spontaneous migraine attacks

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