Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments

Acute chemical ablation of lateral line hair cells is an important tool to understand lateral line-mediated behaviors in free-swimming fish larvae and adults. However, lateral line-mediated behaviors have not been described in fish larvae prior to swim bladder inflation, possibly because single dose...

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Autores principales:	Alexandra Venuto, Timothy Erickson
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	zebrafish lateral line neuromast hair cell ototoxicity toxicity Science Q
Acceso en línea:	https://doaj.org/article/f719df094b0644bcbc0169c1a72848b1
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spelling	oai:doaj.org-article:f719df094b0644bcbc0169c1a72848b12021-11-25T18:10:57ZEvaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments10.3390/life111111802075-1729https://doaj.org/article/f719df094b0644bcbc0169c1a72848b12021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1180https://doaj.org/toc/2075-1729Acute chemical ablation of lateral line hair cells is an important tool to understand lateral line-mediated behaviors in free-swimming fish larvae and adults. However, lateral line-mediated behaviors have not been described in fish larvae prior to swim bladder inflation, possibly because single doses of ototoxin do not effectively silence lateral line function at early developmental stages. To determine whether ototoxins can disrupt lateral line hair cells during early development, we repeatedly exposed zebrafish larvae to the ototoxin neomycin during a 36 h period from 3 to 4 days post-fertilization (dpf). We use simultaneous transgenic and vital dye labeling of hair cells to compare 6-h and 12-h repeated treatment timelines and neomycin concentrations between 0 and 400 µM in terms of larval survival, hair cell death, regeneration, and functional recovery. Following exposure to neomycin, we find that the emergence of newly functional hair cells outpaces cellular regeneration, likely due to the maturation of ototoxin-resistant hair cells that survive treatment. Furthermore, hair cells of 4 dpf larvae exhibit faster recovery compared to 3 dpf larvae. Our data suggest that the rapid functional maturation of ototoxin-resistant hair cells limits the effectiveness of chemical-based methods to disrupt lateral line function. Furthermore, we show that repeated neomycin treatments can continually ablate functional lateral line hair cells between 3 and 4 dpf in larval zebrafish.Alexandra VenutoTimothy EricksonMDPI AGarticlezebrafishlateral lineneuromasthair cellototoxicitytoxicityScienceQENLife, Vol 11, Iss 1180, p 1180 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zebrafish lateral line neuromast hair cell ototoxicity toxicity Science Q
spellingShingle	zebrafish lateral line neuromast hair cell ototoxicity toxicity Science Q Alexandra Venuto Timothy Erickson Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments
description	Acute chemical ablation of lateral line hair cells is an important tool to understand lateral line-mediated behaviors in free-swimming fish larvae and adults. However, lateral line-mediated behaviors have not been described in fish larvae prior to swim bladder inflation, possibly because single doses of ototoxin do not effectively silence lateral line function at early developmental stages. To determine whether ototoxins can disrupt lateral line hair cells during early development, we repeatedly exposed zebrafish larvae to the ototoxin neomycin during a 36 h period from 3 to 4 days post-fertilization (dpf). We use simultaneous transgenic and vital dye labeling of hair cells to compare 6-h and 12-h repeated treatment timelines and neomycin concentrations between 0 and 400 µM in terms of larval survival, hair cell death, regeneration, and functional recovery. Following exposure to neomycin, we find that the emergence of newly functional hair cells outpaces cellular regeneration, likely due to the maturation of ototoxin-resistant hair cells that survive treatment. Furthermore, hair cells of 4 dpf larvae exhibit faster recovery compared to 3 dpf larvae. Our data suggest that the rapid functional maturation of ototoxin-resistant hair cells limits the effectiveness of chemical-based methods to disrupt lateral line function. Furthermore, we show that repeated neomycin treatments can continually ablate functional lateral line hair cells between 3 and 4 dpf in larval zebrafish.
format	article
author	Alexandra Venuto Timothy Erickson
author_facet	Alexandra Venuto Timothy Erickson
author_sort	Alexandra Venuto
title	Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments
title_short	Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments
title_full	Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments
title_fullStr	Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments
title_full_unstemmed	Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments
title_sort	evaluating the death and recovery of lateral line hair cells following repeated neomycin treatments
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f719df094b0644bcbc0169c1a72848b1
work_keys_str_mv	AT alexandravenuto evaluatingthedeathandrecoveryoflaterallinehaircellsfollowingrepeatedneomycintreatments AT timothyerickson evaluatingthedeathandrecoveryoflaterallinehaircellsfollowingrepeatedneomycintreatments
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Evaluating the Death and Recovery of Lateral Line Hair Cells Following Repeated Neomycin Treatments

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