Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis

Polysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in...

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Autores principales:	Min Yang, Fanying Meng, Wen Gu, Lihui Fu, Fan Zhang, Fengjiao Li, Yating Tao, Zhengyang Zhang, Xi Wang, Xingxin Yang, Jingping Li, Jie Yu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Lactobacillus faecis quorum sensing short-chain fatty acids polysaccharides Polygonatum kingianum Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f71a6920b14a4414863ce2fbbe0bed00
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spelling	oai:doaj.org-article:f71a6920b14a4414863ce2fbbe0bed002021-11-17T05:56:21ZInfluence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis1664-302X10.3389/fmicb.2021.758870https://doaj.org/article/f71a6920b14a4414863ce2fbbe0bed002021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.758870/fullhttps://doaj.org/toc/1664-302XPolysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in L. faecis 2-84, and enhanced quorum sensing (QS) signaling. The characteristics of gene sequence were analyzed using genomics approaches, and L. faecis 2-84 was found to encode 18 genes that are closely related to QS and 10 genes related to short-chain fatty acids (SCFAs). Additionally, transcriptome and proteome analysis demonstrated that PS could promote the QS system of L. faecis by enhancing the transcription of oppA gene and expression of oppD protein. PS also regulated the production and metabolism of SCFAs of L. faecis by upregulating the expression of ldh and metE gene and adh2 protein, and downregulating the expression of mvK gene. In conclusion, it was speculated that PS could affect intestinal SCFAs production by affecting the QS system and SCFAs production in L. faecis. The present study implied that PS might have a role in promoting the growth of intestinal probiotics, where the QS system and SCFAs might be two of the important mechanisms for the probiotic activity of PS.Min YangMin YangFanying MengWen GuLihui FuFan ZhangFengjiao LiYating TaoZhengyang ZhangXi WangXingxin YangJingping LiJie YuFrontiers Media S.A.articleLactobacillus faecisquorum sensingshort-chain fatty acidspolysaccharidesPolygonatum kingianumMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lactobacillus faecis quorum sensing short-chain fatty acids polysaccharides Polygonatum kingianum Microbiology QR1-502
spellingShingle	Lactobacillus faecis quorum sensing short-chain fatty acids polysaccharides Polygonatum kingianum Microbiology QR1-502 Min Yang Min Yang Fanying Meng Wen Gu Lihui Fu Fan Zhang Fengjiao Li Yating Tao Zhengyang Zhang Xi Wang Xingxin Yang Jingping Li Jie Yu Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis
description	Polysaccharide is one of the main active ingredients of Polygonatum kingianum, which has been proven to regulate the balance of gut microbiota. For the first time, this study focused on the regulation of polysaccharides from Polygonatum kingianum (PS) on Lactobacillus faecis, a specific probiotic in the intestinal tract. PS effectively promoted the biomass, biofilm and acetic acid production in L. faecis 2-84, and enhanced quorum sensing (QS) signaling. The characteristics of gene sequence were analyzed using genomics approaches, and L. faecis 2-84 was found to encode 18 genes that are closely related to QS and 10 genes related to short-chain fatty acids (SCFAs). Additionally, transcriptome and proteome analysis demonstrated that PS could promote the QS system of L. faecis by enhancing the transcription of oppA gene and expression of oppD protein. PS also regulated the production and metabolism of SCFAs of L. faecis by upregulating the expression of ldh and metE gene and adh2 protein, and downregulating the expression of mvK gene. In conclusion, it was speculated that PS could affect intestinal SCFAs production by affecting the QS system and SCFAs production in L. faecis. The present study implied that PS might have a role in promoting the growth of intestinal probiotics, where the QS system and SCFAs might be two of the important mechanisms for the probiotic activity of PS.
format	article
author	Min Yang Min Yang Fanying Meng Wen Gu Lihui Fu Fan Zhang Fengjiao Li Yating Tao Zhengyang Zhang Xi Wang Xingxin Yang Jingping Li Jie Yu
author_facet	Min Yang Min Yang Fanying Meng Wen Gu Lihui Fu Fan Zhang Fengjiao Li Yating Tao Zhengyang Zhang Xi Wang Xingxin Yang Jingping Li Jie Yu
author_sort	Min Yang
title	Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis
title_short	Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis
title_full	Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis
title_fullStr	Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis
title_full_unstemmed	Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis
title_sort	influence of polysaccharides from polygonatum kingianum on short-chain fatty acid production and quorum sensing in lactobacillus faecis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f71a6920b14a4414863ce2fbbe0bed00
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Influence of Polysaccharides From Polygonatum kingianum on Short-Chain Fatty Acid Production and Quorum Sensing in Lactobacillus faecis

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