Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.

Reptiles represent the crucial phylogenetic group as they were the ancestors of both birds and mammals hence very important to study. The objectives of the present study were to investigate the potential roles of testosterone in the innate immune responses and splenic lymphocyte proliferation in fre...

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Autores principales:	Manish Kumar Tripathi, Ramesh Singh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f724b49d543a4a3dbc0601297d01b39b
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spelling	oai:doaj.org-article:f724b49d543a4a3dbc0601297d01b39b2021-11-25T06:05:41ZDifferential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.1932-620310.1371/journal.pone.0104431https://doaj.org/article/f724b49d543a4a3dbc0601297d01b39b2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25101765/?tool=EBIhttps://doaj.org/toc/1932-6203Reptiles represent the crucial phylogenetic group as they were the ancestors of both birds and mammals hence very important to study. The objectives of the present study were to investigate the potential roles of testosterone in the innate immune responses and splenic lymphocyte proliferation in fresh water snake, Natrix piscator. Animals were mildly anesthetized and spleens were taken out to study the splenic macrophage phagocytosis, super oxide production and nitrite release using in vitro testosterone. Splenic lymphocytes were isolated by density gradient centrifugation and were studied for mitogen induced proliferation in presence of in vitro testosterone. Testosterone suppressed the phagocytosis and nitrite release in a concentration dependent manner. Biphasic suppressive effect of testosterone was observed in superoxide production as judged by reduction of nitroblue tetrazolium salt where salt reduction was suppressed at lower and higher concentrations of testosterone. Mitogen induced splenic lymphocyte proliferation was also suppressed by testosterone. By suppressing immune responses, testosterone may, therefore, act as a physiological mechanism regulating the relative amount of energy invested into either reproductive effort or immunocompetence.Manish Kumar TripathiRamesh SinghPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e104431 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manish Kumar Tripathi Ramesh Singh Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.
description	Reptiles represent the crucial phylogenetic group as they were the ancestors of both birds and mammals hence very important to study. The objectives of the present study were to investigate the potential roles of testosterone in the innate immune responses and splenic lymphocyte proliferation in fresh water snake, Natrix piscator. Animals were mildly anesthetized and spleens were taken out to study the splenic macrophage phagocytosis, super oxide production and nitrite release using in vitro testosterone. Splenic lymphocytes were isolated by density gradient centrifugation and were studied for mitogen induced proliferation in presence of in vitro testosterone. Testosterone suppressed the phagocytosis and nitrite release in a concentration dependent manner. Biphasic suppressive effect of testosterone was observed in superoxide production as judged by reduction of nitroblue tetrazolium salt where salt reduction was suppressed at lower and higher concentrations of testosterone. Mitogen induced splenic lymphocyte proliferation was also suppressed by testosterone. By suppressing immune responses, testosterone may, therefore, act as a physiological mechanism regulating the relative amount of energy invested into either reproductive effort or immunocompetence.
format	article
author	Manish Kumar Tripathi Ramesh Singh
author_facet	Manish Kumar Tripathi Ramesh Singh
author_sort	Manish Kumar Tripathi
title	Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.
title_short	Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.
title_full	Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.
title_fullStr	Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.
title_full_unstemmed	Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.
title_sort	differential suppressive effects of testosterone on immune function in fresh water snake, natrix piscator: an in vitro study.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f724b49d543a4a3dbc0601297d01b39b
work_keys_str_mv	AT manishkumartripathi differentialsuppressiveeffectsoftestosteroneonimmunefunctioninfreshwatersnakenatrixpiscatoraninvitrostudy AT rameshsingh differentialsuppressiveeffectsoftestosteroneonimmunefunctioninfreshwatersnakenatrixpiscatoraninvitrostudy
_version_	1718414199507386368

Differential suppressive effects of testosterone on immune function in fresh water snake, Natrix piscator: an in vitro study.

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