Glial cells modulate retinal cell survival in rotenone-induced neural degeneration

Abstract Administration of the mitochondrial complex I inhibitor rotenone provides an excellent model to study the pathomechanism of oxidative stress-related neural degeneration diseases. In this study, we examined the glial roles in retinal cell survival and degeneration under the rotenone-induced...

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Autores principales:	Hiroshi Tawarayama, Maki Inoue-Yanagimachi, Noriko Himori, Toru Nakazawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f72d7c96af7442a7bc716b68c49d4901
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spelling	oai:doaj.org-article:f72d7c96af7442a7bc716b68c49d49012021-12-02T15:49:45ZGlial cells modulate retinal cell survival in rotenone-induced neural degeneration10.1038/s41598-021-90604-w2045-2322https://doaj.org/article/f72d7c96af7442a7bc716b68c49d49012021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90604-whttps://doaj.org/toc/2045-2322Abstract Administration of the mitochondrial complex I inhibitor rotenone provides an excellent model to study the pathomechanism of oxidative stress-related neural degeneration diseases. In this study, we examined the glial roles in retinal cell survival and degeneration under the rotenone-induced oxidative stress condition. Mouse-derived Müller, microglial (BV-2), and dissociated retinal cells were used for in vitro experiments. Gene expression levels and cell viability were determined using quantitative reverse transcription-polymerase chain reaction and the alamarBlue assay, respectively. Conditioned media were prepared by stimulating glial cells with rotenone. Retinal ganglion cells (RGCs) and inner nuclear layer (INL) were visualized on rat retinal sections by immunohistochemistry and eosin/hematoxylin, respectively. Rotenone dose-dependently induced glial cell death. Treatment with rotenone or rotenone-stimulated glial cell-conditioned media altered gene expression of growth factors and inflammatory cytokines in glial cells. The viability of dissociated retinal cells significantly increased upon culturing in media conditioned with rotenone-stimulated or Müller cell-conditioned media-stimulated BV-2 cells. Furthermore, intravitreal neurotrophin-5 administration prevented the rotenone-induced reduction of RGC number and INL thickness in rats. Thus, glial cells exerted both positive and negative effects on retinal cell survival in rotenone-induced neural degeneration via altered expression of growth factors, especially upregulation of microglia-derived Ntf5, and proinflammatory cytokines.Hiroshi TawarayamaMaki Inoue-YanagimachiNoriko HimoriToru NakazawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroshi Tawarayama Maki Inoue-Yanagimachi Noriko Himori Toru Nakazawa Glial cells modulate retinal cell survival in rotenone-induced neural degeneration
description	Abstract Administration of the mitochondrial complex I inhibitor rotenone provides an excellent model to study the pathomechanism of oxidative stress-related neural degeneration diseases. In this study, we examined the glial roles in retinal cell survival and degeneration under the rotenone-induced oxidative stress condition. Mouse-derived Müller, microglial (BV-2), and dissociated retinal cells were used for in vitro experiments. Gene expression levels and cell viability were determined using quantitative reverse transcription-polymerase chain reaction and the alamarBlue assay, respectively. Conditioned media were prepared by stimulating glial cells with rotenone. Retinal ganglion cells (RGCs) and inner nuclear layer (INL) were visualized on rat retinal sections by immunohistochemistry and eosin/hematoxylin, respectively. Rotenone dose-dependently induced glial cell death. Treatment with rotenone or rotenone-stimulated glial cell-conditioned media altered gene expression of growth factors and inflammatory cytokines in glial cells. The viability of dissociated retinal cells significantly increased upon culturing in media conditioned with rotenone-stimulated or Müller cell-conditioned media-stimulated BV-2 cells. Furthermore, intravitreal neurotrophin-5 administration prevented the rotenone-induced reduction of RGC number and INL thickness in rats. Thus, glial cells exerted both positive and negative effects on retinal cell survival in rotenone-induced neural degeneration via altered expression of growth factors, especially upregulation of microglia-derived Ntf5, and proinflammatory cytokines.
format	article
author	Hiroshi Tawarayama Maki Inoue-Yanagimachi Noriko Himori Toru Nakazawa
author_facet	Hiroshi Tawarayama Maki Inoue-Yanagimachi Noriko Himori Toru Nakazawa
author_sort	Hiroshi Tawarayama
title	Glial cells modulate retinal cell survival in rotenone-induced neural degeneration
title_short	Glial cells modulate retinal cell survival in rotenone-induced neural degeneration
title_full	Glial cells modulate retinal cell survival in rotenone-induced neural degeneration
title_fullStr	Glial cells modulate retinal cell survival in rotenone-induced neural degeneration
title_full_unstemmed	Glial cells modulate retinal cell survival in rotenone-induced neural degeneration
title_sort	glial cells modulate retinal cell survival in rotenone-induced neural degeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f72d7c96af7442a7bc716b68c49d4901
work_keys_str_mv	AT hiroshitawarayama glialcellsmodulateretinalcellsurvivalinrotenoneinducedneuraldegeneration AT makiinoueyanagimachi glialcellsmodulateretinalcellsurvivalinrotenoneinducedneuraldegeneration AT norikohimori glialcellsmodulateretinalcellsurvivalinrotenoneinducedneuraldegeneration AT torunakazawa glialcellsmodulateretinalcellsurvivalinrotenoneinducedneuraldegeneration
_version_	1718385727337660416

Glial cells modulate retinal cell survival in rotenone-induced neural degeneration

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