Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage

Abstract The relationship between cerebral glucose metabolism and glucose transporter expression after intracerebral hemorrhage (ICH) is unclear. Few studies have used positron emission tomography (PET) to explore cerebral glucose metabolism after ICH in rodents. In this study, we produced ICH in mi...

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Autores principales:	Xiaoning Han, Honglei Ren, Ayon Nandi, Xuanjia Fan, Raymond C. Koehler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f72e13ea76f8456e957510c438db6ec6
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spelling	oai:doaj.org-article:f72e13ea76f8456e957510c438db6ec62021-12-02T16:53:18ZAnalysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage10.1038/s41598-021-90216-42045-2322https://doaj.org/article/f72e13ea76f8456e957510c438db6ec62021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90216-4https://doaj.org/toc/2045-2322Abstract The relationship between cerebral glucose metabolism and glucose transporter expression after intracerebral hemorrhage (ICH) is unclear. Few studies have used positron emission tomography (PET) to explore cerebral glucose metabolism after ICH in rodents. In this study, we produced ICH in mice with an intrastriatal injection of collagenase to investigate whether glucose metabolic changes in 18F-fluoro-2-deoxy-D-glucose (FDG)-PET images are associated with expression of glucose transporters (GLUTs) over time. On days 1 and 3 after ICH, the ipsilateral striatum exhibited significant hypometabolism. However, by days 7 and 14, glucose metabolism was significantly higher in the ipsilateral striatum than in the contralateral striatum. The contralateral hemisphere did not show hypermetabolism at any time after ICH. Qualitative immunofluorescence and Western blotting indicated that the expression of GLUT1 in ipsilateral striatum decreased on days 1 and 3 after ICH and gradually returned to baseline by day 21. The 18F-FDG uptake after ICH was associated with expression of GLUT1 but not GLUT3 or GLUT5. Our data suggest that ipsilateral cerebral glucose metabolism decreases in the early stage after ICH and increases progressively in the late stage. Changes in 18F-FDG uptake on PET imaging are associated with the expression of GLUT1 in the ipsilateral striatum.Xiaoning HanHonglei RenAyon NandiXuanjia FanRaymond C. KoehlerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoning Han Honglei Ren Ayon Nandi Xuanjia Fan Raymond C. Koehler Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage
description	Abstract The relationship between cerebral glucose metabolism and glucose transporter expression after intracerebral hemorrhage (ICH) is unclear. Few studies have used positron emission tomography (PET) to explore cerebral glucose metabolism after ICH in rodents. In this study, we produced ICH in mice with an intrastriatal injection of collagenase to investigate whether glucose metabolic changes in 18F-fluoro-2-deoxy-D-glucose (FDG)-PET images are associated with expression of glucose transporters (GLUTs) over time. On days 1 and 3 after ICH, the ipsilateral striatum exhibited significant hypometabolism. However, by days 7 and 14, glucose metabolism was significantly higher in the ipsilateral striatum than in the contralateral striatum. The contralateral hemisphere did not show hypermetabolism at any time after ICH. Qualitative immunofluorescence and Western blotting indicated that the expression of GLUT1 in ipsilateral striatum decreased on days 1 and 3 after ICH and gradually returned to baseline by day 21. The 18F-FDG uptake after ICH was associated with expression of GLUT1 but not GLUT3 or GLUT5. Our data suggest that ipsilateral cerebral glucose metabolism decreases in the early stage after ICH and increases progressively in the late stage. Changes in 18F-FDG uptake on PET imaging are associated with the expression of GLUT1 in the ipsilateral striatum.
format	article
author	Xiaoning Han Honglei Ren Ayon Nandi Xuanjia Fan Raymond C. Koehler
author_facet	Xiaoning Han Honglei Ren Ayon Nandi Xuanjia Fan Raymond C. Koehler
author_sort	Xiaoning Han
title	Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage
title_short	Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage
title_full	Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage
title_fullStr	Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage
title_full_unstemmed	Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage
title_sort	analysis of glucose metabolism by 18f-fdg-pet imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f72e13ea76f8456e957510c438db6ec6
work_keys_str_mv	AT xiaoninghan analysisofglucosemetabolismby18ffdgpetimagingandglucosetransporterexpressioninamousemodelofintracerebralhemorrhage AT hongleiren analysisofglucosemetabolismby18ffdgpetimagingandglucosetransporterexpressioninamousemodelofintracerebralhemorrhage AT ayonnandi analysisofglucosemetabolismby18ffdgpetimagingandglucosetransporterexpressioninamousemodelofintracerebralhemorrhage AT xuanjiafan analysisofglucosemetabolismby18ffdgpetimagingandglucosetransporterexpressioninamousemodelofintracerebralhemorrhage AT raymondckoehler analysisofglucosemetabolismby18ffdgpetimagingandglucosetransporterexpressioninamousemodelofintracerebralhemorrhage
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Analysis of glucose metabolism by 18F-FDG-PET imaging and glucose transporter expression in a mouse model of intracerebral hemorrhage

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