RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE

Abstract. Mycobacteria belonging to Mycobacterium (M.) avium complex (MAC) and M. abscessus complex (MABSC) are the most frequent causes of mycobacteriosis in the world. In the last few years MAC and MABSC taxonomy was rapidly changing due to new molecular methods conveying the possibility to differ...

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Autores principales:	S. Truden, M. Žolnir-Dovč, E. Sodja, M. Starčič Erjavec
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2018
Materias:	nontuberculous mycobacteria mycobacterium abscessus mycobacterium avium macrolide resistance aminoglycoside resistance nation-wide study Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/f72f4a307cca4ef1b1da1083165885de
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spelling	oai:doaj.org-article:f72f4a307cca4ef1b1da1083165885de2021-11-22T07:09:52ZRETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE2220-76192313-739810.15789/2220-7619-2018-4-447-451https://doaj.org/article/f72f4a307cca4ef1b1da1083165885de2018-12-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/838https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. Mycobacteria belonging to Mycobacterium (M.) avium complex (MAC) and M. abscessus complex (MABSC) are the most frequent causes of mycobacteriosis in the world. In the last few years MAC and MABSC taxonomy was rapidly changing due to new molecular methods conveying the possibility to differentiate between species. New techniques are able to identify M. chimaera that was previously recognized as M. intracellulare and also differentiate subspecies of MABSC. Due to their natural habitat, non-tuberculous mycobacteria (NTM) are constantly exposed to various concentrations of antimicrobial drugs and other chemicals and consequently they had developed different mechanisms of resistance. Macrolides and aminoglycosides are frequently used drugs to treat MAC and MABSC infections. The aim of our nation-wide survey was to obtain information about MABSC subspecies prevalence in Slovenia and to assess the percentage of misidentifications of M. chimaera isolates as M. intracellulare in the past. Moreover, the purpose of our study was to reveal, which of the two species M. intracellulare or M. chimaera is clinically more relevant in Slovenia. Further, the aim of the study was to detect mutations in erm(41), rrl and rrs genes, which are known to convey macrolide resistance (erm(41) and rrl) and aminoglycoside resistance (rrs). One hundred and thirty-two Slovenian mycobacterial isolates obtained from the National Mycobacterial Collection that belong to MAC and MABSC were analysed. GenoType NTM-DR was used to differentiate M. intracellulare from M. chimaera and subspecies of MABSC. Our results showed that 48% of previously identified M. intracellulare isolates were actually M. chimaera isolates and that M. abscessus subsp. abscessus was the most frequent subspecies of MABSC. Most of the MABSC isolates carried the inducible macrolide resistance genes (erm(41) and rrl), however none of the isolates of MAC and MABSC had mutations in rrs genes for aminoglycoside resistance.S. TrudenM. Žolnir-DovčE. SodjaM. Starčič ErjavecSankt-Peterburg : NIIÈM imeni Pasteraarticlenontuberculous mycobacteriamycobacterium abscessusmycobacterium aviummacrolide resistanceaminoglycoside resistancenation-wide studyInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 8, Iss 4, Pp 447-451 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	nontuberculous mycobacteria mycobacterium abscessus mycobacterium avium macrolide resistance aminoglycoside resistance nation-wide study Infectious and parasitic diseases RC109-216
spellingShingle	nontuberculous mycobacteria mycobacterium abscessus mycobacterium avium macrolide resistance aminoglycoside resistance nation-wide study Infectious and parasitic diseases RC109-216 S. Truden M. Žolnir-Dovč E. Sodja M. Starčič Erjavec RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE
description	Abstract. Mycobacteria belonging to Mycobacterium (M.) avium complex (MAC) and M. abscessus complex (MABSC) are the most frequent causes of mycobacteriosis in the world. In the last few years MAC and MABSC taxonomy was rapidly changing due to new molecular methods conveying the possibility to differentiate between species. New techniques are able to identify M. chimaera that was previously recognized as M. intracellulare and also differentiate subspecies of MABSC. Due to their natural habitat, non-tuberculous mycobacteria (NTM) are constantly exposed to various concentrations of antimicrobial drugs and other chemicals and consequently they had developed different mechanisms of resistance. Macrolides and aminoglycosides are frequently used drugs to treat MAC and MABSC infections. The aim of our nation-wide survey was to obtain information about MABSC subspecies prevalence in Slovenia and to assess the percentage of misidentifications of M. chimaera isolates as M. intracellulare in the past. Moreover, the purpose of our study was to reveal, which of the two species M. intracellulare or M. chimaera is clinically more relevant in Slovenia. Further, the aim of the study was to detect mutations in erm(41), rrl and rrs genes, which are known to convey macrolide resistance (erm(41) and rrl) and aminoglycoside resistance (rrs). One hundred and thirty-two Slovenian mycobacterial isolates obtained from the National Mycobacterial Collection that belong to MAC and MABSC were analysed. GenoType NTM-DR was used to differentiate M. intracellulare from M. chimaera and subspecies of MABSC. Our results showed that 48% of previously identified M. intracellulare isolates were actually M. chimaera isolates and that M. abscessus subsp. abscessus was the most frequent subspecies of MABSC. Most of the MABSC isolates carried the inducible macrolide resistance genes (erm(41) and rrl), however none of the isolates of MAC and MABSC had mutations in rrs genes for aminoglycoside resistance.
format	article
author	S. Truden M. Žolnir-Dovč E. Sodja M. Starčič Erjavec
author_facet	S. Truden M. Žolnir-Dovč E. Sodja M. Starčič Erjavec
author_sort	S. Truden
title	RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE
title_short	RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE
title_full	RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE
title_fullStr	RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE
title_full_unstemmed	RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE
title_sort	retrospective analysis of slovenian mycobacterium avium complex and mycobacterium abscessus complex isolates and molecular resistance profile
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2018
url	https://doaj.org/article/f72f4a307cca4ef1b1da1083165885de
work_keys_str_mv	AT struden retrospectiveanalysisofslovenianmycobacteriumaviumcomplexandmycobacteriumabscessuscomplexisolatesandmolecularresistanceprofile AT mzolnirdovc retrospectiveanalysisofslovenianmycobacteriumaviumcomplexandmycobacteriumabscessuscomplexisolatesandmolecularresistanceprofile AT esodja retrospectiveanalysisofslovenianmycobacteriumaviumcomplexandmycobacteriumabscessuscomplexisolatesandmolecularresistanceprofile AT mstarcicerjavec retrospectiveanalysisofslovenianmycobacteriumaviumcomplexandmycobacteriumabscessuscomplexisolatesandmolecularresistanceprofile
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RETROSPECTIVE ANALYSIS OF SLOVENIAN MYCOBACTERIUM AVIUM COMPLEX AND MYCOBACTERIUM ABSCESSUS COMPLEX ISOLATES AND MOLECULAR RESISTANCE PROFILE

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