Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1

Abstract Inositol hexakisphosphate kinase-1 (IP6K1) is required for male fertility, but the underlying mechanisms have been elusive. Here, we report that IP6K1 is required for multiple aspects of male germ cell development. This development requires selective interactions between germ cells and Sert...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chenglai Fu, Tomas Rojas, Alfred C. Chin, Weiwei Cheng, Isaac A. Bernstein, Lauren K. Albacarys, William W. Wright, Solomon H. Snyder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f739f70dc2034291be1474b5f19655c4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f739f70dc2034291be1474b5f19655c4
record_format	dspace
spelling	oai:doaj.org-article:f739f70dc2034291be1474b5f19655c42021-12-02T15:08:02ZMultiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-110.1038/s41598-018-25468-82045-2322https://doaj.org/article/f739f70dc2034291be1474b5f19655c42018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25468-8https://doaj.org/toc/2045-2322Abstract Inositol hexakisphosphate kinase-1 (IP6K1) is required for male fertility, but the underlying mechanisms have been elusive. Here, we report that IP6K1 is required for multiple aspects of male germ cell development. This development requires selective interactions between germ cells and Sertoli cells, namely apical ectoplasmic specialization. Spermiation (sperm release) requires tubulobulbar complexes. We found that the apical ectoplasmic specialization and tubulobulbar complexes were poorly formed or disrupted in IP6K1 KOs. Deletion of IP6K1 elicited several aberrations, including: 1, sloughing off of round germ cells; 2, disorientation and malformation of elongating/elongated spermatids; 3, degeneration of acrosomes; 4, defects in germ-Sertoli cell interactions and 5, failure of spermiation. Eventually the sperm cells were not released but phagocytosed by Sertoli cells leading to an absence of sperm in the epididymis.Chenglai FuTomas RojasAlfred C. ChinWeiwei ChengIsaac A. BernsteinLauren K. AlbacarysWilliam W. WrightSolomon H. SnyderNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chenglai Fu Tomas Rojas Alfred C. Chin Weiwei Cheng Isaac A. Bernstein Lauren K. Albacarys William W. Wright Solomon H. Snyder Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1
description	Abstract Inositol hexakisphosphate kinase-1 (IP6K1) is required for male fertility, but the underlying mechanisms have been elusive. Here, we report that IP6K1 is required for multiple aspects of male germ cell development. This development requires selective interactions between germ cells and Sertoli cells, namely apical ectoplasmic specialization. Spermiation (sperm release) requires tubulobulbar complexes. We found that the apical ectoplasmic specialization and tubulobulbar complexes were poorly formed or disrupted in IP6K1 KOs. Deletion of IP6K1 elicited several aberrations, including: 1, sloughing off of round germ cells; 2, disorientation and malformation of elongating/elongated spermatids; 3, degeneration of acrosomes; 4, defects in germ-Sertoli cell interactions and 5, failure of spermiation. Eventually the sperm cells were not released but phagocytosed by Sertoli cells leading to an absence of sperm in the epididymis.
format	article
author	Chenglai Fu Tomas Rojas Alfred C. Chin Weiwei Cheng Isaac A. Bernstein Lauren K. Albacarys William W. Wright Solomon H. Snyder
author_facet	Chenglai Fu Tomas Rojas Alfred C. Chin Weiwei Cheng Isaac A. Bernstein Lauren K. Albacarys William W. Wright Solomon H. Snyder
author_sort	Chenglai Fu
title	Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1
title_short	Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1
title_full	Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1
title_fullStr	Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1
title_full_unstemmed	Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1
title_sort	multiple aspects of male germ cell development and interactions with sertoli cells require inositol hexakisphosphate kinase-1
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f739f70dc2034291be1474b5f19655c4
work_keys_str_mv	AT chenglaifu multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1 AT tomasrojas multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1 AT alfredcchin multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1 AT weiweicheng multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1 AT isaacabernstein multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1 AT laurenkalbacarys multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1 AT williamwwright multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1 AT solomonhsnyder multipleaspectsofmalegermcelldevelopmentandinteractionswithsertolicellsrequireinositolhexakisphosphatekinase1
_version_	1718388293961252864

Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1

Ejemplares similares