Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa

While follicular fluid (FF) is known to enhance the functional properties of spermatozoa, the role of FF-derived extracellular vesicles (EVs) in this respect is unknown. We hypothesized that bovine FF EVs convey signals to spermatozoa supporting sperm viability, inducing sperm capacitation and acros...

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Autores principales:	Mohammad Mehedi Hasan, Qurat Ul Ain Reshi, Freddy Lättekivi, Janeli Viil, Kasun Godakumara, Keerthie Dissanayake, Aneta Andronowska, Ülle Jaakma, Alireza Fazeli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	extracellular vesicles follicular fluid spermatozoa acrosome reaction fertilization Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f75a1ba5f2b54966a3cdaec99201b083
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spelling	oai:doaj.org-article:f75a1ba5f2b54966a3cdaec99201b0832021-11-25T16:47:31ZBovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa10.3390/biology101111542079-7737https://doaj.org/article/f75a1ba5f2b54966a3cdaec99201b0832021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1154https://doaj.org/toc/2079-7737While follicular fluid (FF) is known to enhance the functional properties of spermatozoa, the role of FF-derived extracellular vesicles (EVs) in this respect is unknown. We hypothesized that bovine FF EVs convey signals to spermatozoa supporting sperm viability, inducing sperm capacitation and acrosome reaction. In this study, the effects of bovine FF EVs on sperm functions are evaluated. Irrespective of the size of the follicles which FF EVs had originated from, they were capable of supporting sperm viability, inducing capacitation and acrosome reaction. These effects were specific to the source of bovine FF EVs, as human-cell-line-derived or porcine FF EVs did not affect spermatozoa viability or induced capacitation and acrosome reaction. A minimum of 5 × 10<sup>5</sup> EVs/mL was adequate to maintain sperm viability and induce capacitation and acrosome reaction in spermatozoa. Interestingly, with FF EV trypsin treatment, FF EVs lost their ability to support sperm functions. In conclusion, this study demonstrates that bovine FF EVs can support spermatozoa function and may contribute to a favorable periconceptional microenvironment. This is an important aspect of the interactions between different sexes at the earliest stages of reproduction and helps to understand molecular mechanisms modulating processes such as sperm competition and female cryptic choice.Mohammad Mehedi HasanQurat Ul Ain ReshiFreddy LättekiviJaneli ViilKasun GodakumaraKeerthie DissanayakeAneta AndronowskaÜlle JaakmaAlireza FazeliMDPI AGarticleextracellular vesiclesfollicular fluidspermatozoaacrosome reactionfertilizationBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1154, p 1154 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	extracellular vesicles follicular fluid spermatozoa acrosome reaction fertilization Biology (General) QH301-705.5
spellingShingle	extracellular vesicles follicular fluid spermatozoa acrosome reaction fertilization Biology (General) QH301-705.5 Mohammad Mehedi Hasan Qurat Ul Ain Reshi Freddy Lättekivi Janeli Viil Kasun Godakumara Keerthie Dissanayake Aneta Andronowska Ülle Jaakma Alireza Fazeli Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa
description	While follicular fluid (FF) is known to enhance the functional properties of spermatozoa, the role of FF-derived extracellular vesicles (EVs) in this respect is unknown. We hypothesized that bovine FF EVs convey signals to spermatozoa supporting sperm viability, inducing sperm capacitation and acrosome reaction. In this study, the effects of bovine FF EVs on sperm functions are evaluated. Irrespective of the size of the follicles which FF EVs had originated from, they were capable of supporting sperm viability, inducing capacitation and acrosome reaction. These effects were specific to the source of bovine FF EVs, as human-cell-line-derived or porcine FF EVs did not affect spermatozoa viability or induced capacitation and acrosome reaction. A minimum of 5 × 10<sup>5</sup> EVs/mL was adequate to maintain sperm viability and induce capacitation and acrosome reaction in spermatozoa. Interestingly, with FF EV trypsin treatment, FF EVs lost their ability to support sperm functions. In conclusion, this study demonstrates that bovine FF EVs can support spermatozoa function and may contribute to a favorable periconceptional microenvironment. This is an important aspect of the interactions between different sexes at the earliest stages of reproduction and helps to understand molecular mechanisms modulating processes such as sperm competition and female cryptic choice.
format	article
author	Mohammad Mehedi Hasan Qurat Ul Ain Reshi Freddy Lättekivi Janeli Viil Kasun Godakumara Keerthie Dissanayake Aneta Andronowska Ülle Jaakma Alireza Fazeli
author_facet	Mohammad Mehedi Hasan Qurat Ul Ain Reshi Freddy Lättekivi Janeli Viil Kasun Godakumara Keerthie Dissanayake Aneta Andronowska Ülle Jaakma Alireza Fazeli
author_sort	Mohammad Mehedi Hasan
title	Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa
title_short	Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa
title_full	Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa
title_fullStr	Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa
title_full_unstemmed	Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa
title_sort	bovine follicular fluid derived extracellular vesicles modulate the viability, capacitation and acrosome reaction of bull spermatozoa
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f75a1ba5f2b54966a3cdaec99201b083
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Bovine Follicular Fluid Derived Extracellular Vesicles Modulate the Viability, Capacitation and Acrosome Reaction of Bull Spermatozoa

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