Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)

The Asian honey bee species i.e., Apis cerana (the eastern honey bee), A. dorsata (the giant honey bee), and the western or European honey bee (A. mellifera) collected from Pakistan were studied using partial sequences from two mitochondrial genes (i) the Cytochrome c oxidase I (COI) and (ii) the mi...

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Autor principal:	Khalid Ali Khan
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Apis cerana A. dorsata A. mellifera Molecular analysis DNA markers Genetic analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f75f339158af4d75ac88d650209bb6af
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spelling	oai:doaj.org-article:f75f339158af4d75ac88d650209bb6af2021-11-20T04:56:29ZGenetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)1319-562X10.1016/j.sjbs.2021.07.062https://doaj.org/article/f75f339158af4d75ac88d650209bb6af2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006458https://doaj.org/toc/1319-562XThe Asian honey bee species i.e., Apis cerana (the eastern honey bee), A. dorsata (the giant honey bee), and the western or European honey bee (A. mellifera) collected from Pakistan were studied using partial sequences from two mitochondrial genes (i) the Cytochrome c oxidase I (COI) and (ii) the mitochondrially encoded NADH dehydrogenase 5 (ND5) and then compared with other honey bees sequences (already submitted from different countries around the globe) obtained after the national center for biotechnology information (NCBI). DNA sequences were analyzed employing molecular evolutionary genetics analysis and Kimura 2-parameter model, neighbor-joining method was applied to investigate phylogenetic relationships, and DNA sequence polymorphism was applied to measure the genetic diversity within the genus Apis. The phylogenetic analyses yielded consistent results. Based on COI gene fragment in two Asian and European honey bee species from Pakistan and from other countries showed considerable genetic diversity levels and deviation among the species. While in contrast the phylogenetic analyses based on ND5 gene fragment in Asian and European honey bee species from Pakistan and other countries showed comparatively higher genetic diversity indices and variations than the COI gene. So, in the genus Apis, the mitochondrial ND5 region has shown the possibility to answer the interactions among species. A further detailed work (by linking the analysis of other genomic and mitochondrial genes) is required for good quality solution to establish the concise genetic diversity and interaction among the Apis species. The objective of this study was to explore the extent of genetic differences and phylogenetic links among the three kinds of honey bee species from Pakistan and comparing them with other bee species around the globe.Khalid Ali KhanElsevierarticleApis ceranaA. dorsataA. melliferaMolecular analysisDNA markersGenetic analysisBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6853-6860 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apis cerana A. dorsata A. mellifera Molecular analysis DNA markers Genetic analysis Biology (General) QH301-705.5
spellingShingle	Apis cerana A. dorsata A. mellifera Molecular analysis DNA markers Genetic analysis Biology (General) QH301-705.5 Khalid Ali Khan Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)
description	The Asian honey bee species i.e., Apis cerana (the eastern honey bee), A. dorsata (the giant honey bee), and the western or European honey bee (A. mellifera) collected from Pakistan were studied using partial sequences from two mitochondrial genes (i) the Cytochrome c oxidase I (COI) and (ii) the mitochondrially encoded NADH dehydrogenase 5 (ND5) and then compared with other honey bees sequences (already submitted from different countries around the globe) obtained after the national center for biotechnology information (NCBI). DNA sequences were analyzed employing molecular evolutionary genetics analysis and Kimura 2-parameter model, neighbor-joining method was applied to investigate phylogenetic relationships, and DNA sequence polymorphism was applied to measure the genetic diversity within the genus Apis. The phylogenetic analyses yielded consistent results. Based on COI gene fragment in two Asian and European honey bee species from Pakistan and from other countries showed considerable genetic diversity levels and deviation among the species. While in contrast the phylogenetic analyses based on ND5 gene fragment in Asian and European honey bee species from Pakistan and other countries showed comparatively higher genetic diversity indices and variations than the COI gene. So, in the genus Apis, the mitochondrial ND5 region has shown the possibility to answer the interactions among species. A further detailed work (by linking the analysis of other genomic and mitochondrial genes) is required for good quality solution to establish the concise genetic diversity and interaction among the Apis species. The objective of this study was to explore the extent of genetic differences and phylogenetic links among the three kinds of honey bee species from Pakistan and comparing them with other bee species around the globe.
format	article
author	Khalid Ali Khan
author_facet	Khalid Ali Khan
author_sort	Khalid Ali Khan
title	Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)
title_short	Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)
title_full	Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)
title_fullStr	Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)
title_full_unstemmed	Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)
title_sort	genetic diversity and phylogenetic relationship among the western and the asian honey bees based on two mitochondrial gene segments (coi and nd5)
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f75f339158af4d75ac88d650209bb6af
work_keys_str_mv	AT khalidalikhan geneticdiversityandphylogeneticrelationshipamongthewesternandtheasianhoneybeesbasedontwomitochondrialgenesegmentscoiandnd5
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Genetic diversity and phylogenetic relationship among the western and the Asian honey bees based on two mitochondrial gene segments (COI and ND5)

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