Non-autotrophic methanogens dominate in anaerobic digesters

Abstract Anaerobic digesters are man-made habitats for fermentative and methanogenic microbes, and are characterized by extremely high concentrations of organics. However, little is known about how microbes adapt to such habitats. In the present study, we report phylogenetic, metagenomic, and metatr...

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Autores principales:	Atsushi Kouzuma, Maho Tsutsumi, Shun’ichi Ishii, Yoshiyuki Ueno, Takashi Abe, Kazuya Watanabe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f76728d3071a4fe990b5ea6ff0dc2755
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spelling	oai:doaj.org-article:f76728d3071a4fe990b5ea6ff0dc27552021-12-02T16:06:51ZNon-autotrophic methanogens dominate in anaerobic digesters10.1038/s41598-017-01752-x2045-2322https://doaj.org/article/f76728d3071a4fe990b5ea6ff0dc27552017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01752-xhttps://doaj.org/toc/2045-2322Abstract Anaerobic digesters are man-made habitats for fermentative and methanogenic microbes, and are characterized by extremely high concentrations of organics. However, little is known about how microbes adapt to such habitats. In the present study, we report phylogenetic, metagenomic, and metatranscriptomic analyses of microbiomes in thermophilic packed-bed digesters fed acetate as the major substrate, and we have shown that acetoclastic and hydrogenotrophic methanogens that utilize acetate as a carbon source dominate there. Deep sequencing and precise binning of the metagenomes reconstructed complete genomes for two dominant methanogens affiliated with the genera Methanosarcina and Methanothermobacter, along with 37 draft genomes. The reconstructed Methanosarcina genome was almost identical to that of a thermophilic acetoclastic methanogen Methanosarcina thermophila TM-1, indicating its cosmopolitan distribution in thermophilic digesters. The reconstructed Methanothermobacter (designated as Met2) was closely related to Methanothermobacter tenebrarum, a non-autotrophic hydrogenotrophic methanogen that grows in the presence of acetate. Met2 lacks the Cdh complex required for CO2 fixation, suggesting that it requires organic molecules, such as acetate, as carbon sources. Although the metagenomic analysis also detected autotrophic methanogens, they were less than 1% in abundance of Met2. These results suggested that non-autotrophic methanogens preferentially grow in anaerobic digesters containing high concentrations of organics.Atsushi KouzumaMaho TsutsumiShun’ichi IshiiYoshiyuki UenoTakashi AbeKazuya WatanabeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Atsushi Kouzuma Maho Tsutsumi Shun’ichi Ishii Yoshiyuki Ueno Takashi Abe Kazuya Watanabe Non-autotrophic methanogens dominate in anaerobic digesters
description	Abstract Anaerobic digesters are man-made habitats for fermentative and methanogenic microbes, and are characterized by extremely high concentrations of organics. However, little is known about how microbes adapt to such habitats. In the present study, we report phylogenetic, metagenomic, and metatranscriptomic analyses of microbiomes in thermophilic packed-bed digesters fed acetate as the major substrate, and we have shown that acetoclastic and hydrogenotrophic methanogens that utilize acetate as a carbon source dominate there. Deep sequencing and precise binning of the metagenomes reconstructed complete genomes for two dominant methanogens affiliated with the genera Methanosarcina and Methanothermobacter, along with 37 draft genomes. The reconstructed Methanosarcina genome was almost identical to that of a thermophilic acetoclastic methanogen Methanosarcina thermophila TM-1, indicating its cosmopolitan distribution in thermophilic digesters. The reconstructed Methanothermobacter (designated as Met2) was closely related to Methanothermobacter tenebrarum, a non-autotrophic hydrogenotrophic methanogen that grows in the presence of acetate. Met2 lacks the Cdh complex required for CO2 fixation, suggesting that it requires organic molecules, such as acetate, as carbon sources. Although the metagenomic analysis also detected autotrophic methanogens, they were less than 1% in abundance of Met2. These results suggested that non-autotrophic methanogens preferentially grow in anaerobic digesters containing high concentrations of organics.
format	article
author	Atsushi Kouzuma Maho Tsutsumi Shun’ichi Ishii Yoshiyuki Ueno Takashi Abe Kazuya Watanabe
author_facet	Atsushi Kouzuma Maho Tsutsumi Shun’ichi Ishii Yoshiyuki Ueno Takashi Abe Kazuya Watanabe
author_sort	Atsushi Kouzuma
title	Non-autotrophic methanogens dominate in anaerobic digesters
title_short	Non-autotrophic methanogens dominate in anaerobic digesters
title_full	Non-autotrophic methanogens dominate in anaerobic digesters
title_fullStr	Non-autotrophic methanogens dominate in anaerobic digesters
title_full_unstemmed	Non-autotrophic methanogens dominate in anaerobic digesters
title_sort	non-autotrophic methanogens dominate in anaerobic digesters
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f76728d3071a4fe990b5ea6ff0dc2755
work_keys_str_mv	AT atsushikouzuma nonautotrophicmethanogensdominateinanaerobicdigesters AT mahotsutsumi nonautotrophicmethanogensdominateinanaerobicdigesters AT shunichiishii nonautotrophicmethanogensdominateinanaerobicdigesters AT yoshiyukiueno nonautotrophicmethanogensdominateinanaerobicdigesters AT takashiabe nonautotrophicmethanogensdominateinanaerobicdigesters AT kazuyawatanabe nonautotrophicmethanogensdominateinanaerobicdigesters
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Non-autotrophic methanogens dominate in anaerobic digesters

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