In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity

Abstract Preventing wound infection and retaining an appropriate level of moisture around wounds represent the most critical issues in wound treatment. Towards these ends, special focus has been placed on Bombyx mori cocoons because the protective function of the silkworm cocoon resembles the manner...

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Autores principales:	Kun Yu, Fei Lu, Qing Li, Honglei Chen, Bitao Lu, Jiawei Liu, Zhiquan Li, Fangying Dai, Dayang Wu, Guangqian Lan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7685a81cbc1466bbc1e9c60a9a53ab7
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spelling	oai:doaj.org-article:f7685a81cbc1466bbc1e9c60a9a53ab72021-12-02T15:18:52ZIn situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity10.1038/s41598-017-02270-62045-2322https://doaj.org/article/f7685a81cbc1466bbc1e9c60a9a53ab72017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02270-6https://doaj.org/toc/2045-2322Abstract Preventing wound infection and retaining an appropriate level of moisture around wounds represent the most critical issues in wound treatment. Towards these ends, special focus has been placed on Bombyx mori cocoons because the protective function of the silkworm cocoon resembles the manner in which the skin protects the human body. We have designed a facile technique to develop a novel silkworm cocoon-based wound film (SCWF) wound dressing utilizing a CaCl2-ethanol-H2O solution. To improve the anti-bacterial performance of SCWF, we have incorporated the ability of silk sericin to act as a reducing agent for the conversion of Ag+ to Ag, yielding nanoparticles (AgNPs) linked together by peptide bonds of silkworm cocoon wound film (SCWF-AgNPs). SCWF-AgNP dressing exhibited excellent biocompatibility, anti-bacterial performance, and good extensibility. Furthermore, in vivo experiments indicated that SCWF-AgNP dressing was able to significantly accelerate the healing rate of infected wounds in New Zealand White rabbits and histological examination revealed that it aided in the successful reconstruction of intact and thickened epidermis during 14 days of healing of impaired wound tissue. These results demonstrate that the present approach might shed new light on the design of anti-bacterial materials such as SCWF-AgNPs with promising applications in wound dressing.Kun YuFei LuQing LiHonglei ChenBitao LuJiawei LiuZhiquan LiFangying DaiDayang WuGuangqian LanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kun Yu Fei Lu Qing Li Honglei Chen Bitao Lu Jiawei Liu Zhiquan Li Fangying Dai Dayang Wu Guangqian Lan In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity
description	Abstract Preventing wound infection and retaining an appropriate level of moisture around wounds represent the most critical issues in wound treatment. Towards these ends, special focus has been placed on Bombyx mori cocoons because the protective function of the silkworm cocoon resembles the manner in which the skin protects the human body. We have designed a facile technique to develop a novel silkworm cocoon-based wound film (SCWF) wound dressing utilizing a CaCl2-ethanol-H2O solution. To improve the anti-bacterial performance of SCWF, we have incorporated the ability of silk sericin to act as a reducing agent for the conversion of Ag+ to Ag, yielding nanoparticles (AgNPs) linked together by peptide bonds of silkworm cocoon wound film (SCWF-AgNPs). SCWF-AgNP dressing exhibited excellent biocompatibility, anti-bacterial performance, and good extensibility. Furthermore, in vivo experiments indicated that SCWF-AgNP dressing was able to significantly accelerate the healing rate of infected wounds in New Zealand White rabbits and histological examination revealed that it aided in the successful reconstruction of intact and thickened epidermis during 14 days of healing of impaired wound tissue. These results demonstrate that the present approach might shed new light on the design of anti-bacterial materials such as SCWF-AgNPs with promising applications in wound dressing.
format	article
author	Kun Yu Fei Lu Qing Li Honglei Chen Bitao Lu Jiawei Liu Zhiquan Li Fangying Dai Dayang Wu Guangqian Lan
author_facet	Kun Yu Fei Lu Qing Li Honglei Chen Bitao Lu Jiawei Liu Zhiquan Li Fangying Dai Dayang Wu Guangqian Lan
author_sort	Kun Yu
title	In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity
title_short	In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity
title_full	In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity
title_fullStr	In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity
title_full_unstemmed	In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity
title_sort	in situ assembly of ag nanoparticles (agnps) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f7685a81cbc1466bbc1e9c60a9a53ab7
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In situ assembly of Ag nanoparticles (AgNPs) on porous silkworm cocoon-based wound film: enhanced antimicrobial and wound healing activity

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