Improved skin permeation of methotrexate via nanosized ultradeformable liposomes

Alam Zeb, Omer Salman Qureshi, Hyung-Seo Kim, Ji-Hye Cha, Hoo-Seong Kim, Jin-Ki Kim College of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi, Republic of Korea Abstract: The aim of this study is to investigate methotrexate-entrapped ultradeformab...

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Autores principales:	Zeb A, Qureshi OS, Kim HS, Cha JH, Kim JK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Ultradeformable liposomes Deformability Methotrexate Skin permeation Transdermal delivery Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f7691a41774d4bfca0a0cb2cc58e0815
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spelling	oai:doaj.org-article:f7691a41774d4bfca0a0cb2cc58e08152021-12-02T05:02:12ZImproved skin permeation of methotrexate via nanosized ultradeformable liposomes1178-2013https://doaj.org/article/f7691a41774d4bfca0a0cb2cc58e08152016-08-01T00:00:00Zhttps://www.dovepress.com/improved-skin-permeation-of-methotrexate-via-nanosized-ultradeformable-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Alam Zeb, Omer Salman Qureshi, Hyung-Seo Kim, Ji-Hye Cha, Hoo-Seong Kim, Jin-Ki Kim College of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi, Republic of Korea Abstract: The aim of this study is to investigate methotrexate-entrapped ultradeformable liposomes (MTX-UDLs) for potential transdermal application. MTX-UDLs were prepared by extrusion method with phosphatidylcholine as a bilayer matrix and sodium cholate or Tween 80 as an edge activator. The physicochemical properties of MTX-UDLs were determined in terms of particle size, polydispersity index, zeta potential, and entrapment efficiency. The deformability of MTX-UDLs was compared with that of methotrexate-entrapped conventional liposomes (MTX-CLs) using a steel pressure filter device. The skin permeation of MTX-UDLs was investigated using Franz diffusion cell, and the skin penetration depth of rhodamine 6G-entrapped UDLs was determined by confocal laser scanning microscopy. MTX-UDLs showed a narrow size distribution, with the particle size of ~100 nm. The deformability of MTX-UDLs was two to five times greater than that of MTX-CLs. The skin permeation of MTX-UDLs was significantly improved compared with MTX-CLs and free MTX solution. The optimized UDLs (phosphatidylcholine: Tween 80 =7:3, w/w) showed a higher fluorescence intensity than conventional liposomes at every increment of skin depth. Thus, the optimized UDLs could be promising nanocarriers for systemic delivery of MTX across skin. Keywords: ultradeformable liposomes, deformability, methotrexate, skin permeation, transdermal deliveryZeb AQureshi OSKim HSCha JHKim HSKim JKDove Medical PressarticleUltradeformable liposomesDeformabilityMethotrexateSkin permeationTransdermal deliveryMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 3813-3824 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ultradeformable liposomes Deformability Methotrexate Skin permeation Transdermal delivery Medicine (General) R5-920
spellingShingle	Ultradeformable liposomes Deformability Methotrexate Skin permeation Transdermal delivery Medicine (General) R5-920 Zeb A Qureshi OS Kim HS Cha JH Kim HS Kim JK Improved skin permeation of methotrexate via nanosized ultradeformable liposomes
description	Alam Zeb, Omer Salman Qureshi, Hyung-Seo Kim, Ji-Hye Cha, Hoo-Seong Kim, Jin-Ki Kim College of Pharmacy, Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi, Republic of Korea Abstract: The aim of this study is to investigate methotrexate-entrapped ultradeformable liposomes (MTX-UDLs) for potential transdermal application. MTX-UDLs were prepared by extrusion method with phosphatidylcholine as a bilayer matrix and sodium cholate or Tween 80 as an edge activator. The physicochemical properties of MTX-UDLs were determined in terms of particle size, polydispersity index, zeta potential, and entrapment efficiency. The deformability of MTX-UDLs was compared with that of methotrexate-entrapped conventional liposomes (MTX-CLs) using a steel pressure filter device. The skin permeation of MTX-UDLs was investigated using Franz diffusion cell, and the skin penetration depth of rhodamine 6G-entrapped UDLs was determined by confocal laser scanning microscopy. MTX-UDLs showed a narrow size distribution, with the particle size of ~100 nm. The deformability of MTX-UDLs was two to five times greater than that of MTX-CLs. The skin permeation of MTX-UDLs was significantly improved compared with MTX-CLs and free MTX solution. The optimized UDLs (phosphatidylcholine: Tween 80 =7:3, w/w) showed a higher fluorescence intensity than conventional liposomes at every increment of skin depth. Thus, the optimized UDLs could be promising nanocarriers for systemic delivery of MTX across skin. Keywords: ultradeformable liposomes, deformability, methotrexate, skin permeation, transdermal delivery
format	article
author	Zeb A Qureshi OS Kim HS Cha JH Kim HS Kim JK
author_facet	Zeb A Qureshi OS Kim HS Cha JH Kim HS Kim JK
author_sort	Zeb A
title	Improved skin permeation of methotrexate via nanosized ultradeformable liposomes
title_short	Improved skin permeation of methotrexate via nanosized ultradeformable liposomes
title_full	Improved skin permeation of methotrexate via nanosized ultradeformable liposomes
title_fullStr	Improved skin permeation of methotrexate via nanosized ultradeformable liposomes
title_full_unstemmed	Improved skin permeation of methotrexate via nanosized ultradeformable liposomes
title_sort	improved skin permeation of methotrexate via nanosized ultradeformable liposomes
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/f7691a41774d4bfca0a0cb2cc58e0815
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_version_	1718400807337984000

Improved skin permeation of methotrexate via nanosized ultradeformable liposomes

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