Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs

Abstract Retinal metabolic changes have been suggested to be associated with myopia development. However, little is known about either their identity or time dependent behavior during this sight compromising process. To address these questions, gas chromatography time-of-flight mass spectrometry (GC...

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Autores principales:	Jinglei Yang, Peter S. Reinach, Sen Zhang, Miaozhen Pan, Wenfeng Sun, Bo Liu, Fen Li, Xiaoqing Li, Aihua Zhao, Tianlu Chen, Wei Jia, Jia Qu, Xiangtian Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f76c8f8444d747b7a4f0a7e574f6fe19
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spelling	oai:doaj.org-article:f76c8f8444d747b7a4f0a7e574f6fe192021-12-02T16:06:15ZChanges in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs10.1038/s41598-017-03075-32045-2322https://doaj.org/article/f76c8f8444d747b7a4f0a7e574f6fe192017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03075-3https://doaj.org/toc/2045-2322Abstract Retinal metabolic changes have been suggested to be associated with myopia development. However, little is known about either their identity or time dependent behavior during this sight compromising process. To address these questions, gas chromatography time-of-flight mass spectrometry (GC-TOF/MS) was applied to compare guinea pig retinal metabolite levels in form deprivation (FD) eyes at 3 days and 2 weeks post FD with normal control (NC) eyes. Orthogonal partial least squares (OPLS) models discriminated between time dependent retinal metabolic profiles in the presence and absence of FD. Myopia severity was associated with more metabolic pattern differences in the FD than in the NC eyes. After 3 days of FD, 11 metabolite levels changed and after 2 weeks the number of differences increased to 16. Five metabolites continuously decreased during two weeks of FD. Two-way ANOVA of the changes identified by OPLS indicates that 15 out of the 22 metabolites differences were significant. Taken together, these results suggest that myopia progression is associated with an inverse relationship between increases in glucose accumulation and lipid level decreases in form-deprived guinea pig eyes. Such changes indicate that metabolomic studies are an informative approach to identify time dependent retinal metabolic alterations associated with this disease.Jinglei YangPeter S. ReinachSen ZhangMiaozhen PanWenfeng SunBo LiuFen LiXiaoqing LiAihua ZhaoTianlu ChenWei JiaJia QuXiangtian ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinglei Yang Peter S. Reinach Sen Zhang Miaozhen Pan Wenfeng Sun Bo Liu Fen Li Xiaoqing Li Aihua Zhao Tianlu Chen Wei Jia Jia Qu Xiangtian Zhou Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs
description	Abstract Retinal metabolic changes have been suggested to be associated with myopia development. However, little is known about either their identity or time dependent behavior during this sight compromising process. To address these questions, gas chromatography time-of-flight mass spectrometry (GC-TOF/MS) was applied to compare guinea pig retinal metabolite levels in form deprivation (FD) eyes at 3 days and 2 weeks post FD with normal control (NC) eyes. Orthogonal partial least squares (OPLS) models discriminated between time dependent retinal metabolic profiles in the presence and absence of FD. Myopia severity was associated with more metabolic pattern differences in the FD than in the NC eyes. After 3 days of FD, 11 metabolite levels changed and after 2 weeks the number of differences increased to 16. Five metabolites continuously decreased during two weeks of FD. Two-way ANOVA of the changes identified by OPLS indicates that 15 out of the 22 metabolites differences were significant. Taken together, these results suggest that myopia progression is associated with an inverse relationship between increases in glucose accumulation and lipid level decreases in form-deprived guinea pig eyes. Such changes indicate that metabolomic studies are an informative approach to identify time dependent retinal metabolic alterations associated with this disease.
format	article
author	Jinglei Yang Peter S. Reinach Sen Zhang Miaozhen Pan Wenfeng Sun Bo Liu Fen Li Xiaoqing Li Aihua Zhao Tianlu Chen Wei Jia Jia Qu Xiangtian Zhou
author_facet	Jinglei Yang Peter S. Reinach Sen Zhang Miaozhen Pan Wenfeng Sun Bo Liu Fen Li Xiaoqing Li Aihua Zhao Tianlu Chen Wei Jia Jia Qu Xiangtian Zhou
author_sort	Jinglei Yang
title	Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs
title_short	Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs
title_full	Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs
title_fullStr	Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs
title_full_unstemmed	Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs
title_sort	changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f76c8f8444d747b7a4f0a7e574f6fe19
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Changes in retinal metabolic profiles associated with form deprivation myopia development in guinea pigs

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