Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats

Zeqi Zheng,1,* Lijuan Liu,1,* Kaiwen Zhou,2,* Lu Ding,1,3 Junyi Zeng,1,3 Wan Zhang1,3 1Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 2The First Clinical Medical College, School of Medicine, Nanchang Uni...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zheng Z, Liu L, Zhou K, Ding L, Zeng J, Zhang W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	nano-selenium (nano-se) homocysteine endothelium dysfunction glutathione peroxidase enzymes (gpxs) reactive oxygen species (ros) Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f77f0a6e5610414d9d66192469e74f12
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f77f0a6e5610414d9d66192469e74f12
record_format	dspace
spelling	oai:doaj.org-article:f77f0a6e5610414d9d66192469e74f122021-12-02T10:17:17ZAnti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats1178-2013https://doaj.org/article/f77f0a6e5610414d9d66192469e74f122020-06-01T00:00:00Zhttps://www.dovepress.com/anti-oxidant-and-anti-endothelial-dysfunctional-properties-of-nano-sel-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zeqi Zheng,1,* Lijuan Liu,1,* Kaiwen Zhou,2,* Lu Ding,1,3 Junyi Zeng,1,3 Wan Zhang1,3 1Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 2The First Clinical Medical College, School of Medicine, Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 3Jiangxi Hypertension Research Institute, Nanchang, Jiangxi 330006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wan ZhangDepartment of Cardiology, The First Affiliated Hospital of Nanchang University; Jiangxi Hypertension Research Institute, Nanchang, Jiangxi 330006, People’s Republic of ChinaTel +86-15907086833Fax +86-0791-88692748Email wanzhang@ncu.edu.cnPurpose: Elevation of blood homocysteine (Hcy) level (hyperhomocysteinemia) is a risk factor for cardiovascular disorders and is closely associated with endothelial dysfunction. The present study aims to investigate the protective effect and underlying mechanism of nanoscale selenium (Nano-Se) in Hcy-mediated vascular endothelial cell dysfunction in vitro and in vivo.Materials and Methods: By incubating vascular endothelial cells with exogenous Hcy and generating hyperhomocysteinemic rat model, the effects of Nano-Se on hyperhomocysteinemia-mediated endothelial dysfunction and its essential mechanisms were investigated.Results: Nano-Se inhibited Hcy-induced mitochondrial oxidative damage and apoptosis by preventing the downregulation of glutathione peroxidase enzyme 1 and 4 (GPX1, GPX4) in the vascular endothelial cells, thus effectively prevented the vascular damage in vitro and in vivo in the hyperhomocysteinemic rats. Nano-Se possessed similar protective effects but lower toxicity against Hcy in vascular endothelial cells when compared with other forms of Se.Conclusion: The application of Nano-Se could serve as a novel promising strategy against Hcy-mediated vascular dysfunction with reduced risk of Se toxicity.Keywords: nano-selenium, Nano-Se, homocysteine, endothelium dysfunction, glutathione peroxidase enzymes, GPXs, reactive oxygen species, ROSZheng ZLiu LZhou KDing LZeng JZhang WDove Medical Pressarticlenano-selenium (nano-se)homocysteineendothelium dysfunctionglutathione peroxidase enzymes (gpxs)reactive oxygen species (ros)Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 4501-4521 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	nano-selenium (nano-se) homocysteine endothelium dysfunction glutathione peroxidase enzymes (gpxs) reactive oxygen species (ros) Medicine (General) R5-920
spellingShingle	nano-selenium (nano-se) homocysteine endothelium dysfunction glutathione peroxidase enzymes (gpxs) reactive oxygen species (ros) Medicine (General) R5-920 Zheng Z Liu L Zhou K Ding L Zeng J Zhang W Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats
description	Zeqi Zheng,1,* Lijuan Liu,1,* Kaiwen Zhou,2,* Lu Ding,1,3 Junyi Zeng,1,3 Wan Zhang1,3 1Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 2The First Clinical Medical College, School of Medicine, Nanchang University, Nanchang, Jiangxi 330006, People’s Republic of China; 3Jiangxi Hypertension Research Institute, Nanchang, Jiangxi 330006, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wan ZhangDepartment of Cardiology, The First Affiliated Hospital of Nanchang University; Jiangxi Hypertension Research Institute, Nanchang, Jiangxi 330006, People’s Republic of ChinaTel +86-15907086833Fax +86-0791-88692748Email wanzhang@ncu.edu.cnPurpose: Elevation of blood homocysteine (Hcy) level (hyperhomocysteinemia) is a risk factor for cardiovascular disorders and is closely associated with endothelial dysfunction. The present study aims to investigate the protective effect and underlying mechanism of nanoscale selenium (Nano-Se) in Hcy-mediated vascular endothelial cell dysfunction in vitro and in vivo.Materials and Methods: By incubating vascular endothelial cells with exogenous Hcy and generating hyperhomocysteinemic rat model, the effects of Nano-Se on hyperhomocysteinemia-mediated endothelial dysfunction and its essential mechanisms were investigated.Results: Nano-Se inhibited Hcy-induced mitochondrial oxidative damage and apoptosis by preventing the downregulation of glutathione peroxidase enzyme 1 and 4 (GPX1, GPX4) in the vascular endothelial cells, thus effectively prevented the vascular damage in vitro and in vivo in the hyperhomocysteinemic rats. Nano-Se possessed similar protective effects but lower toxicity against Hcy in vascular endothelial cells when compared with other forms of Se.Conclusion: The application of Nano-Se could serve as a novel promising strategy against Hcy-mediated vascular dysfunction with reduced risk of Se toxicity.Keywords: nano-selenium, Nano-Se, homocysteine, endothelium dysfunction, glutathione peroxidase enzymes, GPXs, reactive oxygen species, ROS
format	article
author	Zheng Z Liu L Zhou K Ding L Zeng J Zhang W
author_facet	Zheng Z Liu L Zhou K Ding L Zeng J Zhang W
author_sort	Zheng Z
title	Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats
title_short	Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats
title_full	Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats
title_fullStr	Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats
title_full_unstemmed	Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats
title_sort	anti-oxidant and anti-endothelial dysfunctional properties of nano-selenium in vitro and in vivo of hyperhomocysteinemic rats
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/f77f0a6e5610414d9d66192469e74f12
work_keys_str_mv	AT zhengz antioxidantandantiendothelialdysfunctionalpropertiesofnanoseleniuminvitroandinvivoofhyperhomocysteinemicrats AT liul antioxidantandantiendothelialdysfunctionalpropertiesofnanoseleniuminvitroandinvivoofhyperhomocysteinemicrats AT zhouk antioxidantandantiendothelialdysfunctionalpropertiesofnanoseleniuminvitroandinvivoofhyperhomocysteinemicrats AT dingl antioxidantandantiendothelialdysfunctionalpropertiesofnanoseleniuminvitroandinvivoofhyperhomocysteinemicrats AT zengj antioxidantandantiendothelialdysfunctionalpropertiesofnanoseleniuminvitroandinvivoofhyperhomocysteinemicrats AT zhangw antioxidantandantiendothelialdysfunctionalpropertiesofnanoseleniuminvitroandinvivoofhyperhomocysteinemicrats
_version_	1718397450277879808

Anti-Oxidant and Anti-Endothelial Dysfunctional Properties of Nano-Selenium in vitro and in vivo of Hyperhomocysteinemic Rats

Ejemplares similares