Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.

Pollen is the most common aeroallergen to cause seasonal conjunctivitis. The result of allergen exposure is a strong Th2-mediated response along with conjunctival mast cell degranulation and eosinophilic infiltration. Oleanolic acid (OA) is natural a triterpene that displays strong anti-inflammatory...

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Autores principales:	Claudia Córdova, Beatriz Gutiérrez, Carmen Martínez-García, Rubén Martín, Patricia Gallego-Muñoz, Marita Hernández, María L Nieto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f785d9e1a727431b81537c95b7418212
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spelling	oai:doaj.org-article:f785d9e1a727431b81537c95b74182122021-11-18T08:25:08ZOleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.1932-620310.1371/journal.pone.0091282https://doaj.org/article/f785d9e1a727431b81537c95b74182122014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24699261/?tool=EBIhttps://doaj.org/toc/1932-6203Pollen is the most common aeroallergen to cause seasonal conjunctivitis. The result of allergen exposure is a strong Th2-mediated response along with conjunctival mast cell degranulation and eosinophilic infiltration. Oleanolic acid (OA) is natural a triterpene that displays strong anti-inflammatory and immunomodulatory properties being an active anti-allergic molecule on hypersensitivity reaction models. However, its effect on inflammatory ocular disorders including conjunctivitis, has not yet been addressed. Hence, using a Ragweed pollen (RWP)-specific allergic conjunctivitis (EAC) mouse model we study here whether OA could modify responses associated to allergic processes. We found that OA treatment restricted mast cell degranulation and infiltration of eosinophils in conjunctival tissue and decreased allergen-specific Igs levels in EAC mice. Th2-type cytokines, secreted phospholipase A2 type-IIA (sPLA2-IIA), and chemokines levels were also significantly diminished in the conjunctiva and serum of OA-treated EAC mice. Moreover, OA treatment also suppressed RWP-specific T-cell proliferation. In vitro studies, on relevant cells of the allergic process, revealed that OA reduced the proliferative and migratory response, as well as the synthesis of proinflammatory mediators on EoL-1 eosinophils and RBL-2H3 mast cells exposed to allergic and/or crucial inflammatory stimuli such as RWP, sPLA2-IIA or eotaxin. Taken together, these findings demonstrate the beneficial activity of OA in ocular allergic processes and may provide a new intervention strategy and potential therapy for allergic diseases.Claudia CórdovaBeatriz GutiérrezCarmen Martínez-GarcíaRubén MartínPatricia Gallego-MuñozMarita HernándezMaría L NietoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e91282 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claudia Córdova Beatriz Gutiérrez Carmen Martínez-García Rubén Martín Patricia Gallego-Muñoz Marita Hernández María L Nieto Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.
description	Pollen is the most common aeroallergen to cause seasonal conjunctivitis. The result of allergen exposure is a strong Th2-mediated response along with conjunctival mast cell degranulation and eosinophilic infiltration. Oleanolic acid (OA) is natural a triterpene that displays strong anti-inflammatory and immunomodulatory properties being an active anti-allergic molecule on hypersensitivity reaction models. However, its effect on inflammatory ocular disorders including conjunctivitis, has not yet been addressed. Hence, using a Ragweed pollen (RWP)-specific allergic conjunctivitis (EAC) mouse model we study here whether OA could modify responses associated to allergic processes. We found that OA treatment restricted mast cell degranulation and infiltration of eosinophils in conjunctival tissue and decreased allergen-specific Igs levels in EAC mice. Th2-type cytokines, secreted phospholipase A2 type-IIA (sPLA2-IIA), and chemokines levels were also significantly diminished in the conjunctiva and serum of OA-treated EAC mice. Moreover, OA treatment also suppressed RWP-specific T-cell proliferation. In vitro studies, on relevant cells of the allergic process, revealed that OA reduced the proliferative and migratory response, as well as the synthesis of proinflammatory mediators on EoL-1 eosinophils and RBL-2H3 mast cells exposed to allergic and/or crucial inflammatory stimuli such as RWP, sPLA2-IIA or eotaxin. Taken together, these findings demonstrate the beneficial activity of OA in ocular allergic processes and may provide a new intervention strategy and potential therapy for allergic diseases.
format	article
author	Claudia Córdova Beatriz Gutiérrez Carmen Martínez-García Rubén Martín Patricia Gallego-Muñoz Marita Hernández María L Nieto
author_facet	Claudia Córdova Beatriz Gutiérrez Carmen Martínez-García Rubén Martín Patricia Gallego-Muñoz Marita Hernández María L Nieto
author_sort	Claudia Córdova
title	Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.
title_short	Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.
title_full	Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.
title_fullStr	Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.
title_full_unstemmed	Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.
title_sort	oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f785d9e1a727431b81537c95b7418212
work_keys_str_mv	AT claudiacordova oleanolicacidcontrolsallergicandinflammatoryresponsesinexperimentalallergicconjunctivitis AT beatrizgutierrez oleanolicacidcontrolsallergicandinflammatoryresponsesinexperimentalallergicconjunctivitis AT carmenmartinezgarcia oleanolicacidcontrolsallergicandinflammatoryresponsesinexperimentalallergicconjunctivitis AT rubenmartin oleanolicacidcontrolsallergicandinflammatoryresponsesinexperimentalallergicconjunctivitis AT patriciagallegomunoz oleanolicacidcontrolsallergicandinflammatoryresponsesinexperimentalallergicconjunctivitis AT maritahernandez oleanolicacidcontrolsallergicandinflammatoryresponsesinexperimentalallergicconjunctivitis AT marialnieto oleanolicacidcontrolsallergicandinflammatoryresponsesinexperimentalallergicconjunctivitis
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Oleanolic acid controls allergic and inflammatory responses in experimental allergic conjunctivitis.

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