The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR

Purpose. Recurrent miscarriage applies to pregnancy loss expulsion of the fetus within the first 24 weeks of pregnancy. This study is aimed at comparatively investigating the sera of women with RM with those who have no record of miscarriages to identify if there were any metabolite and metabolic pa...

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Autores principales:	Mahbobeh Latifimehr, Ali Asghar Rastegari, Zahra Zamani, Pezhman Fard Esfahani, Leila Nazari
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/f78c0de1a3274042bce2f8036bfc39f4
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spelling	oai:doaj.org-article:f78c0de1a3274042bce2f8036bfc39f42021-11-15T01:20:06ZThe Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR2314-614110.1155/2021/3422138https://doaj.org/article/f78c0de1a3274042bce2f8036bfc39f42021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/3422138https://doaj.org/toc/2314-6141Purpose. Recurrent miscarriage applies to pregnancy loss expulsion of the fetus within the first 24 weeks of pregnancy. This study is aimed at comparatively investigating the sera of women with RM with those who have no record of miscarriages to identify if there were any metabolite and metabolic pathway differences using 1H NMR spectroscopy. Methods. Serum samples were collected from women with RM (n=30) and those who had no records of RM (n=30) to obtain metabolomics information. 1H NMR spectroscopy was carried out on the samples using Carr Purcell Meiboom Gill spin echo; also, Partial Least Squares Discriminant Analysis was performed in MATLAB software using the ProMetab program to obtain the classifying chemical shifts; the metabolites were identified by using the Human Metabolome Database (HMDB) in both the experimental and control groups. The pathway analysis option of the Metaboanalyst.ca website was used to identify the changed metabolic pathways. Results. The results of the study revealed that 14 metabolites were different in the patients with RM. Moreover, the pathway analysis showed that taurine and hypotaurine metabolism along with phenylalanine, tyrosine, and tryptophan biosynthesis was significantly different in patients with RM. Conclusion. The present study proposes that any alteration in the above metabolic pathways might lead to metabolic dysfunctions which may result in a higher probability of RM.Mahbobeh LatifimehrAli Asghar RastegariZahra ZamaniPezhman Fard EsfahaniLeila NazariHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Mahbobeh Latifimehr Ali Asghar Rastegari Zahra Zamani Pezhman Fard Esfahani Leila Nazari The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR
description	Purpose. Recurrent miscarriage applies to pregnancy loss expulsion of the fetus within the first 24 weeks of pregnancy. This study is aimed at comparatively investigating the sera of women with RM with those who have no record of miscarriages to identify if there were any metabolite and metabolic pathway differences using 1H NMR spectroscopy. Methods. Serum samples were collected from women with RM (n=30) and those who had no records of RM (n=30) to obtain metabolomics information. 1H NMR spectroscopy was carried out on the samples using Carr Purcell Meiboom Gill spin echo; also, Partial Least Squares Discriminant Analysis was performed in MATLAB software using the ProMetab program to obtain the classifying chemical shifts; the metabolites were identified by using the Human Metabolome Database (HMDB) in both the experimental and control groups. The pathway analysis option of the Metaboanalyst.ca website was used to identify the changed metabolic pathways. Results. The results of the study revealed that 14 metabolites were different in the patients with RM. Moreover, the pathway analysis showed that taurine and hypotaurine metabolism along with phenylalanine, tyrosine, and tryptophan biosynthesis was significantly different in patients with RM. Conclusion. The present study proposes that any alteration in the above metabolic pathways might lead to metabolic dysfunctions which may result in a higher probability of RM.
format	article
author	Mahbobeh Latifimehr Ali Asghar Rastegari Zahra Zamani Pezhman Fard Esfahani Leila Nazari
author_facet	Mahbobeh Latifimehr Ali Asghar Rastegari Zahra Zamani Pezhman Fard Esfahani Leila Nazari
author_sort	Mahbobeh Latifimehr
title	The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR
title_short	The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR
title_full	The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR
title_fullStr	The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR
title_full_unstemmed	The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR
title_sort	investigation of metabonomic pathways of serum of iranian women with recurrent miscarriage using 1h nmr
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/f78c0de1a3274042bce2f8036bfc39f4
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The Investigation of Metabonomic Pathways of Serum of Iranian Women with Recurrent Miscarriage Using 1H NMR

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