Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases
The response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely impacted by the level of virus exposure and status of the host immunity. The nature of protection shown by direct asymptomatic contacts of coronavirus disease 2019 (COVID-19)-positive patients is quite intriguing...
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2021
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oai:doaj.org-article:f78d523583884c08bef03f3242ab53952021-12-01T01:47:26ZSingle-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases1664-322410.3389/fimmu.2021.733539https://doaj.org/article/f78d523583884c08bef03f3242ab53952021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.733539/fullhttps://doaj.org/toc/1664-3224The response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely impacted by the level of virus exposure and status of the host immunity. The nature of protection shown by direct asymptomatic contacts of coronavirus disease 2019 (COVID-19)-positive patients is quite intriguing. In this study, we have characterized the antibody titer, SARS-CoV-2 surrogate virus neutralization, cytokine levels, single-cell T-cell receptor (TCR), and B-cell receptor (BCR) profiling in asymptomatic direct contacts, infected cases, and controls. We observed significant increase in antibodies with neutralizing amplitude in asymptomatic contacts along with cytokines such as Eotaxin, granulocyte-colony stimulating factor (G-CSF), interleukin 7 (IL-7), migration inhibitory factor (MIF), and macrophage inflammatory protein-1α (MIP-1α). Upon single-cell RNA (scRNA) sequencing, we explored the dynamics of the adaptive immune response in few representative asymptomatic close contacts and COVID-19-infected patients. We reported direct asymptomatic contacts to have decreased CD4+ naive T cells with concomitant increase in CD4+ memory and CD8+ Temra cells along with expanded clonotypes compared to infected patients. Noticeable proportions of class switched memory B cells were also observed in them. Overall, these findings gave an insight into the nature of protection in asymptomatic contacts.Kaushik SenKaushik SenSudeshna DattaArup GhoshArup GhoshAtimukta JhaAtimukta JhaAbdul AhadAbdul AhadSanchari ChatterjeeSanchari ChatterjeeSandhya SuranjikaSandhya SuranjikaSoumya SenguptaSoumya SenguptaGargee BhattacharyaGargee BhattacharyaOmprakash ShriwasOmprakash ShriwasKiran AvulaKiran AvulaJayasingh KshatriPunit PrasadRajeeb SwainAjay K. ParidaSunil K. RaghavSunil K. RaghavSunil K. RaghavFrontiers Media S.A.articleSARS-CoV-2antibody titerscRNA-seqscBCR-seqscTCR-seqcytokine levelsImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
SARS-CoV-2 antibody titer scRNA-seq scBCR-seq scTCR-seq cytokine levels Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
SARS-CoV-2 antibody titer scRNA-seq scBCR-seq scTCR-seq cytokine levels Immunologic diseases. Allergy RC581-607 Kaushik Sen Kaushik Sen Sudeshna Datta Arup Ghosh Arup Ghosh Atimukta Jha Atimukta Jha Abdul Ahad Abdul Ahad Sanchari Chatterjee Sanchari Chatterjee Sandhya Suranjika Sandhya Suranjika Soumya Sengupta Soumya Sengupta Gargee Bhattacharya Gargee Bhattacharya Omprakash Shriwas Omprakash Shriwas Kiran Avula Kiran Avula Jayasingh Kshatri Punit Prasad Rajeeb Swain Ajay K. Parida Sunil K. Raghav Sunil K. Raghav Sunil K. Raghav Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases |
| description |
The response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely impacted by the level of virus exposure and status of the host immunity. The nature of protection shown by direct asymptomatic contacts of coronavirus disease 2019 (COVID-19)-positive patients is quite intriguing. In this study, we have characterized the antibody titer, SARS-CoV-2 surrogate virus neutralization, cytokine levels, single-cell T-cell receptor (TCR), and B-cell receptor (BCR) profiling in asymptomatic direct contacts, infected cases, and controls. We observed significant increase in antibodies with neutralizing amplitude in asymptomatic contacts along with cytokines such as Eotaxin, granulocyte-colony stimulating factor (G-CSF), interleukin 7 (IL-7), migration inhibitory factor (MIF), and macrophage inflammatory protein-1α (MIP-1α). Upon single-cell RNA (scRNA) sequencing, we explored the dynamics of the adaptive immune response in few representative asymptomatic close contacts and COVID-19-infected patients. We reported direct asymptomatic contacts to have decreased CD4+ naive T cells with concomitant increase in CD4+ memory and CD8+ Temra cells along with expanded clonotypes compared to infected patients. Noticeable proportions of class switched memory B cells were also observed in them. Overall, these findings gave an insight into the nature of protection in asymptomatic contacts. |
| format |
article |
| author |
Kaushik Sen Kaushik Sen Sudeshna Datta Arup Ghosh Arup Ghosh Atimukta Jha Atimukta Jha Abdul Ahad Abdul Ahad Sanchari Chatterjee Sanchari Chatterjee Sandhya Suranjika Sandhya Suranjika Soumya Sengupta Soumya Sengupta Gargee Bhattacharya Gargee Bhattacharya Omprakash Shriwas Omprakash Shriwas Kiran Avula Kiran Avula Jayasingh Kshatri Punit Prasad Rajeeb Swain Ajay K. Parida Sunil K. Raghav Sunil K. Raghav Sunil K. Raghav |
| author_facet |
Kaushik Sen Kaushik Sen Sudeshna Datta Arup Ghosh Arup Ghosh Atimukta Jha Atimukta Jha Abdul Ahad Abdul Ahad Sanchari Chatterjee Sanchari Chatterjee Sandhya Suranjika Sandhya Suranjika Soumya Sengupta Soumya Sengupta Gargee Bhattacharya Gargee Bhattacharya Omprakash Shriwas Omprakash Shriwas Kiran Avula Kiran Avula Jayasingh Kshatri Punit Prasad Rajeeb Swain Ajay K. Parida Sunil K. Raghav Sunil K. Raghav Sunil K. Raghav |
| author_sort |
Kaushik Sen |
| title |
Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases |
| title_short |
Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases |
| title_full |
Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases |
| title_fullStr |
Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases |
| title_full_unstemmed |
Single-Cell Immunogenomic Approach Identified SARS-CoV-2 Protective Immune Signatures in Asymptomatic Direct Contacts of COVID-19 Cases |
| title_sort |
single-cell immunogenomic approach identified sars-cov-2 protective immune signatures in asymptomatic direct contacts of covid-19 cases |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/f78d523583884c08bef03f3242ab5395 |
| work_keys_str_mv |
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