Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.

<h4>Background</h4>Our previous studies suggested that deoxyschizandrin (DSD) and schisantherin A (STA) may have cardioprotective effects, but information in this regard is lacking. Therefore, we explored the protective role of DSD and STA in myocardial ischemia-reperfusion (I/R) injury....

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Autores principales:	Ruimiao Chang, Yong Li, Xingxin Yang, Yuan Yue, Lili Dou, Yanwei Wang, Weifang Zhang, Xiaoni Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f7934d4ad92a48119553113c5692b9a4
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spelling	oai:doaj.org-article:f7934d4ad92a48119553113c5692b9a42021-11-18T07:48:44ZProtective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.1932-620310.1371/journal.pone.0061590https://doaj.org/article/f7934d4ad92a48119553113c5692b9a42013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23620773/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Our previous studies suggested that deoxyschizandrin (DSD) and schisantherin A (STA) may have cardioprotective effects, but information in this regard is lacking. Therefore, we explored the protective role of DSD and STA in myocardial ischemia-reperfusion (I/R) injury.<h4>Methodology/principal findings</h4>Anesthetized male rats were treated once with DSD and STA (each 40 µmol/kg) through the tail vein after 45 min of ischemia, followed by 2-h reperfusion. Cardiac function, infarct size, biochemical markers, histopathology and apoptosis were measured and mRNA expression of gp91 (phox) in myocardial tissue assessed by RT-PCR. Neonatal rat cardiomyocytes were pretreated with DSD and STA and then damaged by H2O2. Cell apoptosis was tested by a flow cytometric assay. Compared with the I/R group: (i) DSD and STA could significantly reduce the abnormalities of LVSP, LVEDP, ±dp/dtmax and arrhythmias, thereby showing their protective roles in cardiac function; (ii) DSD and STA could significantly attenuate the infarct size and MDA release while increasing SOD activity, suggesting a role in reducing myocardial injury; (iii) tissue morphology and myocardial textual analysis revealed that DSD and STA mitigated changes in myocardial histopathology; (iv) DSD and STA decreased apoptosis (33.56±2.58% to 10.28±2.80% and 10.98±1.99%, respectively) and caspase-3 activity in the myocardium (0.62±0.02 OD/mg to 0.38±0.02 OD/mg and 0.32±0.02 OD/mg, respectively), showing their protective effects upon cardiomyocytes; and (v) DSD and STA had similar protective effects on I/R injury as those seen with the positive control metoprolol. In vitro, DSD and STA could significantly decrease the apoptosis of neonatal cardiomyocytes.<h4>Conclusions/significance</h4>These data suggest that DSD and STA can protect against myocardial I/R injury. The underlining mechanism may be related to their role in inhibiting cardiomyocyte apoptosis.Ruimiao ChangYong LiXingxin YangYuan YueLili DouYanwei WangWeifang ZhangXiaoni LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61590 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ruimiao Chang Yong Li Xingxin Yang Yuan Yue Lili Dou Yanwei Wang Weifang Zhang Xiaoni Li Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.
description	<h4>Background</h4>Our previous studies suggested that deoxyschizandrin (DSD) and schisantherin A (STA) may have cardioprotective effects, but information in this regard is lacking. Therefore, we explored the protective role of DSD and STA in myocardial ischemia-reperfusion (I/R) injury.<h4>Methodology/principal findings</h4>Anesthetized male rats were treated once with DSD and STA (each 40 µmol/kg) through the tail vein after 45 min of ischemia, followed by 2-h reperfusion. Cardiac function, infarct size, biochemical markers, histopathology and apoptosis were measured and mRNA expression of gp91 (phox) in myocardial tissue assessed by RT-PCR. Neonatal rat cardiomyocytes were pretreated with DSD and STA and then damaged by H2O2. Cell apoptosis was tested by a flow cytometric assay. Compared with the I/R group: (i) DSD and STA could significantly reduce the abnormalities of LVSP, LVEDP, ±dp/dtmax and arrhythmias, thereby showing their protective roles in cardiac function; (ii) DSD and STA could significantly attenuate the infarct size and MDA release while increasing SOD activity, suggesting a role in reducing myocardial injury; (iii) tissue morphology and myocardial textual analysis revealed that DSD and STA mitigated changes in myocardial histopathology; (iv) DSD and STA decreased apoptosis (33.56±2.58% to 10.28±2.80% and 10.98±1.99%, respectively) and caspase-3 activity in the myocardium (0.62±0.02 OD/mg to 0.38±0.02 OD/mg and 0.32±0.02 OD/mg, respectively), showing their protective effects upon cardiomyocytes; and (v) DSD and STA had similar protective effects on I/R injury as those seen with the positive control metoprolol. In vitro, DSD and STA could significantly decrease the apoptosis of neonatal cardiomyocytes.<h4>Conclusions/significance</h4>These data suggest that DSD and STA can protect against myocardial I/R injury. The underlining mechanism may be related to their role in inhibiting cardiomyocyte apoptosis.
format	article
author	Ruimiao Chang Yong Li Xingxin Yang Yuan Yue Lili Dou Yanwei Wang Weifang Zhang Xiaoni Li
author_facet	Ruimiao Chang Yong Li Xingxin Yang Yuan Yue Lili Dou Yanwei Wang Weifang Zhang Xiaoni Li
author_sort	Ruimiao Chang
title	Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.
title_short	Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.
title_full	Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.
title_fullStr	Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.
title_full_unstemmed	Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.
title_sort	protective role of deoxyschizandrin and schisantherin a against myocardial ischemia-reperfusion injury in rats.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f7934d4ad92a48119553113c5692b9a4
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Protective role of deoxyschizandrin and schisantherin A against myocardial ischemia-reperfusion injury in rats.

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